Experiment: H2 N-free with 10mM D-glucosamine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS00665 and MMJJ_RS00670 are separated by 576 nucleotides MMJJ_RS00670 and MMJJ_RS00675 overlap by 35 nucleotides MMJJ_RS00675 and MMJJ_RS00680 are separated by 16 nucleotides
MMJJ_RS00665: MMJJ_RS00665 - lysine 2,3-aminomutase, at 128,959 to 130,260
_RS00665
MMJJ_RS00670: MMJJ_RS00670 - nitrogen fixation, at 130,837 to 131,157
_RS00670
MMJJ_RS00675: MMJJ_RS00675 - nitrogenase, at 131,123 to 132,499
_RS00675
MMJJ_RS00680: MMJJ_RS00680 - nitrogenase iron-molybdenum cofactor biosynthesis protein NifE, at 132,516 to 133,958
_RS00680
Position (kb) 131
132
133 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 130.140 kb on - strand at 130.140 kb on - strand at 130.140 kb on - strand at 130.141 kb on + strand at 130.141 kb on + strand at 130.153 kb on + strand at 130.178 kb on - strand at 130.179 kb on + strand at 130.226 kb on - strand at 130.227 kb on + strand at 130.232 kb on + strand at 130.232 kb on + strand at 130.234 kb on - strand at 130.236 kb on - strand at 130.237 kb on + strand at 130.249 kb on - strand at 130.271 kb on - strand at 130.271 kb on - strand at 130.272 kb on + strand at 130.272 kb on + strand at 130.317 kb on - strand at 130.317 kb on - strand at 130.317 kb on - strand at 130.317 kb on - strand at 130.317 kb on - strand at 130.318 kb on + strand at 130.319 kb on - strand at 130.319 kb on - strand at 130.319 kb on - strand at 130.320 kb on + strand at 130.320 kb on + strand at 130.320 kb on + strand at 130.343 kb on - strand at 130.344 kb on + strand at 130.394 kb on - strand at 130.394 kb on - strand at 130.394 kb on - strand at 130.394 kb on - strand at 130.395 kb on + strand at 130.440 kb on - strand at 130.440 kb on - strand at 130.440 kb on - strand at 130.440 kb on - strand at 130.441 kb on + strand at 130.441 kb on + strand at 130.441 kb on + strand at 130.444 kb on + strand at 130.445 kb on - strand at 130.445 kb on - strand at 130.445 kb on - strand at 130.445 kb on - strand at 130.450 kb on - strand at 130.450 kb on - strand at 130.450 kb on - strand at 130.451 kb on + strand at 130.451 kb on + strand at 130.451 kb on + strand at 130.488 kb on + strand at 130.488 kb on + strand at 130.489 kb on - strand at 130.489 kb on - strand at 130.489 kb on - strand at 130.489 kb on - strand at 130.490 kb on + strand at 130.498 kb on - strand at 130.521 kb on - strand at 130.521 kb on - strand at 130.522 kb on + strand at 130.522 kb on + strand at 130.522 kb on + strand at 130.525 kb on - strand at 130.525 kb on - strand at 130.530 kb on - strand at 130.531 kb on + strand at 130.534 kb on - strand at 130.553 kb on + strand at 130.563 kb on + strand at 130.572 kb on + strand at 130.572 kb on + strand at 130.597 kb on - strand at 130.640 kb on - strand at 130.640 kb on - strand at 130.640 kb on - strand at 130.641 kb on + strand at 130.656 kb on + strand at 130.701 kb on - strand at 130.729 kb on - strand at 130.735 kb on - strand at 130.891 kb on - strand, within MMJJ_RS00670 at 130.985 kb on - strand, within MMJJ_RS00670 at 130.985 kb on - strand, within MMJJ_RS00670 at 130.986 kb on + strand, within MMJJ_RS00670 at 130.986 kb on + strand, within MMJJ_RS00670 at 130.986 kb on + strand, within MMJJ_RS00670 at 131.011 kb on - strand, within MMJJ_RS00670 at 131.011 kb on - strand, within MMJJ_RS00670 at 131.011 kb on - strand, within MMJJ_RS00670 at 131.011 kb on - strand, within MMJJ_RS00670 at 131.012 kb on + strand, within MMJJ_RS00670 at 131.012 kb on + strand, within MMJJ_RS00670 at 131.012 kb on + strand, within MMJJ_RS00670 at 131.012 kb on + strand, within MMJJ_RS00670 at 131.012 kb on + strand, within MMJJ_RS00670 at 131.013 kb on - strand, within MMJJ_RS00670 at 131.013 kb on - strand, within MMJJ_RS00670 at 131.116 kb on - strand, within MMJJ_RS00670 at 131.116 kb on - strand, within MMJJ_RS00670 at 131.117 kb on + strand, within MMJJ_RS00670 at 131.117 kb on + strand, within MMJJ_RS00670 at 131.163 kb on + strand at 131.170 kb on - strand at 131.170 kb on - strand at 131.180 kb on - strand at 131.221 kb on - strand at 131.221 kb on - strand at 131.221 kb on - strand at 131.221 kb on - strand at 131.222 kb on + strand at 131.230 kb on - strand at 131.230 kb on - strand at 131.230 kb on - strand at 131.230 kb on - strand at 131.231 kb on + strand at 131.231 kb on + strand at 131.231 kb on + strand at 131.231 kb on + strand at 131.233 kb on - strand at 131.233 kb on - strand at 131.233 kb on - strand at 131.233 kb on - strand at 131.233 kb on - strand at 131.233 kb on - strand at 131.234 kb on + strand at 131.234 kb on + strand at 131.237 kb on - strand at 131.237 kb on - strand at 131.237 kb on - strand at 131.237 kb on - strand at 131.238 kb on + strand at 131.238 kb on + strand at 131.246 kb on + strand at 131.246 kb on + strand at 131.246 kb on + strand at 131.257 kb on - strand at 131.258 kb on + strand at 131.259 kb on - strand at 131.298 kb on - strand, within MMJJ_RS00675 at 131.299 kb on + strand, within MMJJ_RS00675 at 131.299 kb on + strand, within MMJJ_RS00675 at 131.299 kb on + strand, within MMJJ_RS00675 at 131.317 kb on - strand, within MMJJ_RS00675 at 131.318 kb on + strand, within MMJJ_RS00675 at 131.432 kb on - strand, within MMJJ_RS00675 at 131.461 kb on - strand, within MMJJ_RS00675 at 131.565 kb on + strand, within MMJJ_RS00675 at 131.656 kb on - strand, within MMJJ_RS00675 at 131.656 kb on - strand, within MMJJ_RS00675 at 131.656 kb on - strand, within MMJJ_RS00675 at 131.660 kb on - strand, within MMJJ_RS00675 at 131.660 kb on - strand, within MMJJ_RS00675 at 131.672 kb on - strand, within MMJJ_RS00675 at 131.686 kb on - strand, within MMJJ_RS00675 at 131.732 kb on - strand, within MMJJ_RS00675 at 131.733 kb on + strand, within MMJJ_RS00675 at 131.738 kb on - strand, within MMJJ_RS00675 at 131.742 kb on - strand, within MMJJ_RS00675 at 131.743 kb on + strand, within MMJJ_RS00675 at 131.806 kb on - strand, within MMJJ_RS00675 at 131.912 kb on + strand, within MMJJ_RS00675 at 131.945 kb on - strand, within MMJJ_RS00675 at 131.945 kb on - strand, within MMJJ_RS00675 at 132.018 kb on + strand, within MMJJ_RS00675 at 132.068 kb on + strand, within MMJJ_RS00675 at 132.097 kb on - strand, within MMJJ_RS00675 at 132.097 kb on - strand, within MMJJ_RS00675 at 132.097 kb on - strand, within MMJJ_RS00675 at 132.098 kb on + strand, within MMJJ_RS00675 at 132.098 kb on + strand, within MMJJ_RS00675 at 132.098 kb on + strand, within MMJJ_RS00675 at 132.098 kb on + strand, within MMJJ_RS00675 at 132.098 kb on + strand, within MMJJ_RS00675 at 132.098 kb on + strand, within MMJJ_RS00675 at 132.098 kb on + strand, within MMJJ_RS00675 at 132.098 kb on + strand, within MMJJ_RS00675 at 132.103 kb on - strand, within MMJJ_RS00675 at 132.103 kb on - strand, within MMJJ_RS00675 at 132.203 kb on + strand, within MMJJ_RS00675 at 132.215 kb on + strand, within MMJJ_RS00675 at 132.215 kb on + strand, within MMJJ_RS00675 at 132.219 kb on + strand, within MMJJ_RS00675 at 132.224 kb on - strand, within MMJJ_RS00675 at 132.225 kb on + strand, within MMJJ_RS00675 at 132.227 kb on - strand, within MMJJ_RS00675 at 132.372 kb on - strand at 132.372 kb on - strand at 132.372 kb on - strand at 132.372 kb on - strand at 132.373 kb on + strand at 132.373 kb on + strand at 132.399 kb on + strand at 132.399 kb on + strand at 132.399 kb on + strand at 132.438 kb on + strand at 132.438 kb on + strand at 132.458 kb on - strand at 132.458 kb on - strand at 132.459 kb on + strand at 132.459 kb on + strand at 132.473 kb on + strand at 132.502 kb on - strand at 132.688 kb on + strand, within MMJJ_RS00680 at 132.700 kb on - strand, within MMJJ_RS00680 at 132.717 kb on - strand, within MMJJ_RS00680 at 132.718 kb on + strand, within MMJJ_RS00680 at 132.718 kb on + strand, within MMJJ_RS00680 at 132.751 kb on + strand, within MMJJ_RS00680 at 132.755 kb on - strand, within MMJJ_RS00680 at 132.755 kb on - strand, within MMJJ_RS00680 at 132.755 kb on - strand, within MMJJ_RS00680 at 132.756 kb on + strand, within MMJJ_RS00680 at 132.756 kb on + strand, within MMJJ_RS00680 at 132.776 kb on - strand, within MMJJ_RS00680 at 132.777 kb on + strand, within MMJJ_RS00680 at 132.887 kb on + strand, within MMJJ_RS00680 at 132.888 kb on - strand, within MMJJ_RS00680 at 132.889 kb on + strand, within MMJJ_RS00680 at 132.903 kb on + strand, within MMJJ_RS00680 at 132.941 kb on - strand, within MMJJ_RS00680 at 133.035 kb on + strand, within MMJJ_RS00680 at 133.104 kb on - strand, within MMJJ_RS00680 at 133.108 kb on - strand, within MMJJ_RS00680 at 133.109 kb on + strand, within MMJJ_RS00680 at 133.134 kb on - strand, within MMJJ_RS00680 at 133.134 kb on - strand, within MMJJ_RS00680 at 133.136 kb on - strand, within MMJJ_RS00680 at 133.136 kb on - strand, within MMJJ_RS00680 at 133.137 kb on + strand, within MMJJ_RS00680 at 133.141 kb on + strand, within MMJJ_RS00680 at 133.146 kb on + strand, within MMJJ_RS00680 at 133.146 kb on + strand, within MMJJ_RS00680 at 133.190 kb on - strand, within MMJJ_RS00680 at 133.203 kb on - strand, within MMJJ_RS00680 at 133.203 kb on - strand, within MMJJ_RS00680 at 133.227 kb on - strand, within MMJJ_RS00680 at 133.235 kb on - strand, within MMJJ_RS00680 at 133.275 kb on - strand, within MMJJ_RS00680 at 133.277 kb on - strand, within MMJJ_RS00680 at 133.277 kb on - strand, within MMJJ_RS00680 at 133.277 kb on - strand, within MMJJ_RS00680 at 133.284 kb on + strand, within MMJJ_RS00680 at 133.323 kb on - strand, within MMJJ_RS00680 at 133.323 kb on - strand, within MMJJ_RS00680 at 133.327 kb on - strand, within MMJJ_RS00680 at 133.374 kb on + strand, within MMJJ_RS00680 at 133.439 kb on - strand, within MMJJ_RS00680 at 133.440 kb on + strand, within MMJJ_RS00680 at 133.452 kb on + strand, within MMJJ_RS00680 at 133.452 kb on + strand, within MMJJ_RS00680 at 133.473 kb on - strand, within MMJJ_RS00680 at 133.473 kb on - strand, within MMJJ_RS00680 at 133.476 kb on - strand, within MMJJ_RS00680 at 133.497 kb on - strand, within MMJJ_RS00680
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM D-glucosamine remove 130,140 - -0.2 130,140 - -0.1 130,140 - -0.4 130,141 + +1.0 130,141 + -1.0 130,153 + -1.8 130,178 - -0.2 130,179 + +0.4 130,226 - +0.2 130,227 + -1.5 130,232 + -0.8 130,232 + -0.2 130,234 - -1.1 130,236 - -2.0 130,237 + -1.5 130,249 - +0.4 130,271 - -1.6 130,271 - -1.5 130,272 + -1.2 130,272 + -2.2 130,317 - +1.7 130,317 - +2.7 130,317 - -2.6 130,317 - -0.9 130,317 - -1.5 130,318 + -2.2 130,319 - -2.5 130,319 - -0.7 130,319 - +0.1 130,320 + +0.1 130,320 + -0.9 130,320 + +0.4 130,343 - +1.3 130,344 + -1.8 130,394 - -0.7 130,394 - -1.0 130,394 - -1.9 130,394 - -1.9 130,395 + +0.8 130,440 - +0.7 130,440 - -1.3 130,440 - -1.9 130,440 - -1.7 130,441 + +0.7 130,441 + -0.4 130,441 + -1.3 130,444 + -0.8 130,445 - +0.8 130,445 - +0.9 130,445 - +0.4 130,445 - +0.4 130,450 - -1.2 130,450 - +1.1 130,450 - +1.2 130,451 + -0.1 130,451 + +0.5 130,451 + -1.7 130,488 + -0.4 130,488 + +0.8 130,489 - +0.0 130,489 - -2.0 130,489 - +0.6 130,489 - +1.6 130,490 + +0.4 130,498 - +0.1 130,521 - +0.7 130,521 - -0.1 130,522 + +1.4 130,522 + +0.4 130,522 + +0.8 130,525 - -1.1 130,525 - -0.1 130,530 - +0.7 130,531 + -0.9 130,534 - +3.0 130,553 + -0.2 130,563 + -1.2 130,572 + -0.6 130,572 + +0.5 130,597 - +1.4 130,640 - -0.4 130,640 - -1.8 130,640 - -0.1 130,641 + -0.6 130,656 + -2.1 130,701 - -1.9 130,729 - +0.4 130,735 - -1.9 130,891 - MMJJ_RS00670 0.17 -1.2 130,985 - MMJJ_RS00670 0.46 +1.0 130,985 - MMJJ_RS00670 0.46 -1.0 130,986 + MMJJ_RS00670 0.46 -1.0 130,986 + MMJJ_RS00670 0.46 +0.9 130,986 + MMJJ_RS00670 0.46 -1.4 131,011 - MMJJ_RS00670 0.54 +0.2 131,011 - MMJJ_RS00670 0.54 +1.2 131,011 - MMJJ_RS00670 0.54 -0.4 131,011 - MMJJ_RS00670 0.54 +0.0 131,012 + MMJJ_RS00670 0.55 +1.2 131,012 + MMJJ_RS00670 0.55 +0.9 131,012 + MMJJ_RS00670 0.55 +0.5 131,012 + MMJJ_RS00670 0.55 -0.7 131,012 + MMJJ_RS00670 0.55 -0.2 131,013 - MMJJ_RS00670 0.55 -0.8 131,013 - MMJJ_RS00670 0.55 +0.0 131,116 - MMJJ_RS00670 0.87 +0.6 131,116 - MMJJ_RS00670 0.87 +0.7 131,117 + MMJJ_RS00670 0.87 +0.3 131,117 + MMJJ_RS00670 0.87 -0.6 131,163 + -0.8 131,170 - -0.5 131,170 - +0.1 131,180 - -0.5 131,221 - +0.5 131,221 - +2.0 131,221 - -0.8 131,221 - -0.8 131,222 + -0.8 131,230 - -1.7 131,230 - -1.2 131,230 - -4.5 131,230 - -2.3 131,231 + -1.9 131,231 + -2.1 131,231 + -3.3 131,231 + -4.1 131,233 - -2.7 131,233 - +0.0 131,233 - -3.3 131,233 - -2.5 131,233 - -2.4 131,233 - -2.1 131,234 + -0.6 131,234 + -4.4 131,237 - -3.5 131,237 - -3.7 131,237 - -3.5 131,237 - -5.1 131,238 + -1.2 131,238 + -3.3 131,246 + -1.6 131,246 + -2.6 131,246 + -4.4 131,257 - -1.6 131,258 + -3.2 131,259 - -2.4 131,298 - MMJJ_RS00675 0.13 -1.8 131,299 + MMJJ_RS00675 0.13 +2.0 131,299 + MMJJ_RS00675 0.13 -1.4 131,299 + MMJJ_RS00675 0.13 -3.4 131,317 - MMJJ_RS00675 0.14 -2.3 131,318 + MMJJ_RS00675 0.14 -2.3 131,432 - MMJJ_RS00675 0.22 -2.1 131,461 - MMJJ_RS00675 0.25 -2.4 131,565 + MMJJ_RS00675 0.32 -3.7 131,656 - MMJJ_RS00675 0.39 -3.0 131,656 - MMJJ_RS00675 0.39 -3.3 131,656 - MMJJ_RS00675 0.39 -3.1 131,660 - MMJJ_RS00675 0.39 -3.1 131,660 - MMJJ_RS00675 0.39 -3.9 131,672 - MMJJ_RS00675 0.40 -3.7 131,686 - MMJJ_RS00675 0.41 -2.3 131,732 - MMJJ_RS00675 0.44 -1.4 131,733 + MMJJ_RS00675 0.44 -2.4 131,738 - MMJJ_RS00675 0.45 -0.8 131,742 - MMJJ_RS00675 0.45 -3.3 131,743 + MMJJ_RS00675 0.45 -2.6 131,806 - MMJJ_RS00675 0.50 -0.3 131,912 + MMJJ_RS00675 0.57 -2.3 131,945 - MMJJ_RS00675 0.60 -2.6 131,945 - MMJJ_RS00675 0.60 -3.2 132,018 + MMJJ_RS00675 0.65 -4.8 132,068 + MMJJ_RS00675 0.69 -3.5 132,097 - MMJJ_RS00675 0.71 -3.6 132,097 - MMJJ_RS00675 0.71 -2.1 132,097 - MMJJ_RS00675 0.71 -2.7 132,098 + MMJJ_RS00675 0.71 -2.4 132,098 + MMJJ_RS00675 0.71 -2.7 132,098 + MMJJ_RS00675 0.71 -2.5 132,098 + MMJJ_RS00675 0.71 -1.2 132,098 + MMJJ_RS00675 0.71 -2.5 132,098 + MMJJ_RS00675 0.71 -0.2 132,098 + MMJJ_RS00675 0.71 -2.5 132,098 + MMJJ_RS00675 0.71 -0.6 132,103 - MMJJ_RS00675 0.71 -0.7 132,103 - MMJJ_RS00675 0.71 -3.2 132,203 + MMJJ_RS00675 0.78 -2.0 132,215 + MMJJ_RS00675 0.79 -2.8 132,215 + MMJJ_RS00675 0.79 -1.7 132,219 + MMJJ_RS00675 0.80 -3.3 132,224 - MMJJ_RS00675 0.80 -3.2 132,225 + MMJJ_RS00675 0.80 -2.6 132,227 - MMJJ_RS00675 0.80 -1.2 132,372 - -2.2 132,372 - -2.8 132,372 - -2.1 132,372 - -1.0 132,373 + -2.8 132,373 + -3.9 132,399 + -2.5 132,399 + -2.6 132,399 + -1.0 132,438 + -1.9 132,438 + -1.7 132,458 - -1.2 132,458 - -2.0 132,459 + -3.1 132,459 + -3.6 132,473 + -1.6 132,502 - -2.7 132,688 + MMJJ_RS00680 0.12 -1.4 132,700 - MMJJ_RS00680 0.13 -3.4 132,717 - MMJJ_RS00680 0.14 -1.1 132,718 + MMJJ_RS00680 0.14 -1.0 132,718 + MMJJ_RS00680 0.14 -2.2 132,751 + MMJJ_RS00680 0.16 -2.2 132,755 - MMJJ_RS00680 0.17 -2.7 132,755 - MMJJ_RS00680 0.17 -2.8 132,755 - MMJJ_RS00680 0.17 -2.0 132,756 + MMJJ_RS00680 0.17 -2.4 132,756 + MMJJ_RS00680 0.17 -3.6 132,776 - MMJJ_RS00680 0.18 -3.7 132,777 + MMJJ_RS00680 0.18 -3.5 132,887 + MMJJ_RS00680 0.26 -0.6 132,888 - MMJJ_RS00680 0.26 -2.5 132,889 + MMJJ_RS00680 0.26 -1.9 132,903 + MMJJ_RS00680 0.27 -3.0 132,941 - MMJJ_RS00680 0.29 -2.3 133,035 + MMJJ_RS00680 0.36 -3.3 133,104 - MMJJ_RS00680 0.41 -1.0 133,108 - MMJJ_RS00680 0.41 -1.3 133,109 + MMJJ_RS00680 0.41 +0.5 133,134 - MMJJ_RS00680 0.43 -2.3 133,134 - MMJJ_RS00680 0.43 -2.6 133,136 - MMJJ_RS00680 0.43 -2.4 133,136 - MMJJ_RS00680 0.43 -1.8 133,137 + MMJJ_RS00680 0.43 -3.1 133,141 + MMJJ_RS00680 0.43 -2.6 133,146 + MMJJ_RS00680 0.44 -5.1 133,146 + MMJJ_RS00680 0.44 -1.3 133,190 - MMJJ_RS00680 0.47 -2.4 133,203 - MMJJ_RS00680 0.48 -2.0 133,203 - MMJJ_RS00680 0.48 -2.6 133,227 - MMJJ_RS00680 0.49 -3.7 133,235 - MMJJ_RS00680 0.50 -2.9 133,275 - MMJJ_RS00680 0.53 -4.5 133,277 - MMJJ_RS00680 0.53 -1.7 133,277 - MMJJ_RS00680 0.53 -3.4 133,277 - MMJJ_RS00680 0.53 -3.0 133,284 + MMJJ_RS00680 0.53 -2.3 133,323 - MMJJ_RS00680 0.56 -1.4 133,323 - MMJJ_RS00680 0.56 -4.3 133,327 - MMJJ_RS00680 0.56 -2.3 133,374 + MMJJ_RS00680 0.59 -3.7 133,439 - MMJJ_RS00680 0.64 -1.4 133,440 + MMJJ_RS00680 0.64 -1.5 133,452 + MMJJ_RS00680 0.65 -3.9 133,452 + MMJJ_RS00680 0.65 -2.4 133,473 - MMJJ_RS00680 0.66 -4.5 133,473 - MMJJ_RS00680 0.66 -1.6 133,476 - MMJJ_RS00680 0.67 -1.8 133,497 - MMJJ_RS00680 0.68 -1.4
Or see this region's nucleotide sequence