Experiment: H2 N-free with 10mM D-glucosamine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS00095 and MMJJ_RS00100 are separated by 77 nucleotides MMJJ_RS00100 and MMJJ_RS00105 are separated by 50 nucleotides MMJJ_RS00105 and MMJJ_RS00110 are separated by 21 nucleotides
MMJJ_RS00095: MMJJ_RS00095 - permease, at 17,952 to 18,809
_RS00095
MMJJ_RS00100: MMJJ_RS00100 - hypothetical protein, at 18,887 to 19,300
_RS00100
MMJJ_RS00105: MMJJ_RS00105 - adenylosuccinate lyase, at 19,351 to 20,700
_RS00105
MMJJ_RS00110: MMJJ_RS00110 - ATP-dependent DNA ligase, at 20,722 to 22,443
_RS00110
Position (kb) 19
20
21 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 18.363 kb on - strand, within MMJJ_RS00095 at 18.506 kb on - strand, within MMJJ_RS00095 at 18.524 kb on + strand, within MMJJ_RS00095 at 18.526 kb on - strand at 18.527 kb on + strand, within MMJJ_RS00095 at 18.527 kb on + strand, within MMJJ_RS00095 at 18.548 kb on - strand, within MMJJ_RS00095 at 18.556 kb on + strand, within MMJJ_RS00095 at 18.586 kb on - strand, within MMJJ_RS00095 at 18.620 kb on - strand, within MMJJ_RS00095 at 18.621 kb on + strand, within MMJJ_RS00095 at 18.621 kb on + strand, within MMJJ_RS00095 at 18.657 kb on + strand, within MMJJ_RS00095 at 18.671 kb on + strand, within MMJJ_RS00095 at 18.688 kb on - strand, within MMJJ_RS00095 at 18.688 kb on - strand, within MMJJ_RS00095 at 18.688 kb on - strand, within MMJJ_RS00095 at 18.689 kb on + strand, within MMJJ_RS00095 at 18.723 kb on - strand, within MMJJ_RS00095 at 18.748 kb on + strand at 18.748 kb on + strand at 18.760 kb on - strand at 18.760 kb on - strand at 18.764 kb on - strand at 18.764 kb on - strand at 18.778 kb on - strand at 18.791 kb on + strand at 18.794 kb on + strand at 18.797 kb on - strand at 18.797 kb on - strand at 18.797 kb on - strand at 18.798 kb on + strand at 18.798 kb on + strand at 18.798 kb on + strand at 18.798 kb on + strand at 18.807 kb on - strand at 18.838 kb on - strand at 18.838 kb on - strand at 18.838 kb on - strand at 18.838 kb on - strand at 18.838 kb on - strand at 18.839 kb on + strand at 18.841 kb on + strand at 18.842 kb on - strand at 18.842 kb on - strand at 18.842 kb on - strand at 18.842 kb on - strand at 18.842 kb on - strand at 18.842 kb on - strand at 18.842 kb on - strand at 18.842 kb on - strand at 18.843 kb on + strand at 18.843 kb on + strand at 18.843 kb on + strand at 18.843 kb on + strand at 18.870 kb on - strand at 18.918 kb on - strand at 18.919 kb on + strand at 19.056 kb on - strand, within MMJJ_RS00100 at 19.060 kb on - strand, within MMJJ_RS00100 at 19.087 kb on + strand, within MMJJ_RS00100 at 19.100 kb on + strand, within MMJJ_RS00100 at 19.112 kb on + strand, within MMJJ_RS00100 at 19.128 kb on - strand, within MMJJ_RS00100 at 19.128 kb on - strand, within MMJJ_RS00100 at 19.129 kb on + strand, within MMJJ_RS00100 at 19.129 kb on + strand, within MMJJ_RS00100 at 19.132 kb on - strand, within MMJJ_RS00100 at 19.132 kb on - strand, within MMJJ_RS00100 at 19.144 kb on - strand, within MMJJ_RS00100 at 19.155 kb on + strand, within MMJJ_RS00100 at 19.160 kb on + strand, within MMJJ_RS00100 at 19.205 kb on + strand, within MMJJ_RS00100 at 19.212 kb on - strand, within MMJJ_RS00100 at 19.212 kb on - strand, within MMJJ_RS00100 at 19.216 kb on - strand, within MMJJ_RS00100 at 19.217 kb on + strand, within MMJJ_RS00100 at 19.221 kb on - strand, within MMJJ_RS00100 at 19.227 kb on + strand, within MMJJ_RS00100 at 19.233 kb on - strand, within MMJJ_RS00100 at 19.256 kb on - strand, within MMJJ_RS00100 at 19.281 kb on - strand at 19.281 kb on - strand at 19.283 kb on - strand at 19.283 kb on - strand at 19.287 kb on + strand at 19.287 kb on + strand at 19.326 kb on + strand at 19.331 kb on - strand at 19.365 kb on - strand at 19.537 kb on + strand, within MMJJ_RS00105 at 21.095 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM D-glucosamine remove 18,363 - MMJJ_RS00095 0.48 +0.3 18,506 - MMJJ_RS00095 0.65 -0.2 18,524 + MMJJ_RS00095 0.67 -0.9 18,526 - +0.4 18,527 + MMJJ_RS00095 0.67 -0.8 18,527 + MMJJ_RS00095 0.67 +0.8 18,548 - MMJJ_RS00095 0.69 +0.5 18,556 + MMJJ_RS00095 0.70 -1.1 18,586 - MMJJ_RS00095 0.74 +0.4 18,620 - MMJJ_RS00095 0.78 -0.4 18,621 + MMJJ_RS00095 0.78 -0.1 18,621 + MMJJ_RS00095 0.78 +0.7 18,657 + MMJJ_RS00095 0.82 -0.8 18,671 + MMJJ_RS00095 0.84 -0.6 18,688 - MMJJ_RS00095 0.86 -1.0 18,688 - MMJJ_RS00095 0.86 +0.8 18,688 - MMJJ_RS00095 0.86 -0.7 18,689 + MMJJ_RS00095 0.86 -0.9 18,723 - MMJJ_RS00095 0.90 +1.0 18,748 + +0.1 18,748 + +1.9 18,760 - +0.2 18,760 - +1.0 18,764 - +0.6 18,764 - +0.1 18,778 - -1.0 18,791 + +0.8 18,794 + -0.6 18,797 - +1.1 18,797 - +1.7 18,797 - -0.5 18,798 + +1.9 18,798 + -1.1 18,798 + -0.2 18,798 + -0.3 18,807 - -1.9 18,838 - +0.8 18,838 - -0.2 18,838 - -1.4 18,838 - -0.3 18,838 - -0.7 18,839 + -0.0 18,841 + +0.5 18,842 - +0.5 18,842 - +1.0 18,842 - +0.6 18,842 - +5.0 18,842 - -0.6 18,842 - +0.8 18,842 - +0.7 18,842 - +1.1 18,843 + -3.3 18,843 + +0.4 18,843 + -0.0 18,843 + +0.9 18,870 - -0.4 18,918 - -1.2 18,919 + -0.6 19,056 - MMJJ_RS00100 0.41 +0.6 19,060 - MMJJ_RS00100 0.42 +0.3 19,087 + MMJJ_RS00100 0.48 +0.6 19,100 + MMJJ_RS00100 0.51 +0.1 19,112 + MMJJ_RS00100 0.54 -0.3 19,128 - MMJJ_RS00100 0.58 -0.5 19,128 - MMJJ_RS00100 0.58 -0.2 19,129 + MMJJ_RS00100 0.58 -1.9 19,129 + MMJJ_RS00100 0.58 -1.7 19,132 - MMJJ_RS00100 0.59 -0.1 19,132 - MMJJ_RS00100 0.59 -0.9 19,144 - MMJJ_RS00100 0.62 -0.5 19,155 + MMJJ_RS00100 0.65 -0.3 19,160 + MMJJ_RS00100 0.66 +0.4 19,205 + MMJJ_RS00100 0.77 -0.5 19,212 - MMJJ_RS00100 0.79 -0.5 19,212 - MMJJ_RS00100 0.79 +0.0 19,216 - MMJJ_RS00100 0.79 -0.7 19,217 + MMJJ_RS00100 0.80 +1.2 19,221 - MMJJ_RS00100 0.81 +1.0 19,227 + MMJJ_RS00100 0.82 -0.6 19,233 - MMJJ_RS00100 0.84 +0.7 19,256 - MMJJ_RS00100 0.89 -0.9 19,281 - +1.7 19,281 - +0.1 19,283 - -0.7 19,283 - +0.6 19,287 + -0.3 19,287 + -0.5 19,326 + -0.5 19,331 - +0.2 19,365 - -0.7 19,537 + MMJJ_RS00105 0.14 +0.0 21,095 + +0.4
Or see this region's nucleotide sequence