Experiment: R2A with lithium chloride 15.625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS17965 and LRK54_RS17970 are separated by 82 nucleotides LRK54_RS17970 and LRK54_RS17975 are separated by 154 nucleotides
LRK54_RS17965: LRK54_RS17965 - heat-inducible transcriptional repressor HrcA, at 3,921,335 to 3,922,405
_RS17965
LRK54_RS17970: LRK54_RS17970 - nucleotide exchange factor GrpE, at 3,922,488 to 3,923,021
_RS17970
LRK54_RS17975: LRK54_RS17975 - molecular chaperone DnaK, at 3,923,176 to 3,925,095
_RS17975
Position (kb)
3922
3923
3924 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 3921.658 kb on + strand, within LRK54_RS17965 at 3921.658 kb on + strand, within LRK54_RS17965 at 3921.658 kb on + strand, within LRK54_RS17965 at 3921.658 kb on + strand, within LRK54_RS17965 at 3921.658 kb on + strand, within LRK54_RS17965 at 3921.658 kb on + strand, within LRK54_RS17965 at 3921.659 kb on - strand, within LRK54_RS17965 at 3921.659 kb on - strand, within LRK54_RS17965 at 3921.763 kb on + strand, within LRK54_RS17965 at 3921.763 kb on + strand, within LRK54_RS17965 at 3921.763 kb on + strand, within LRK54_RS17965 at 3921.763 kb on + strand, within LRK54_RS17965 at 3921.764 kb on - strand, within LRK54_RS17965 at 3921.893 kb on + strand, within LRK54_RS17965 at 3921.893 kb on + strand, within LRK54_RS17965 at 3921.893 kb on + strand, within LRK54_RS17965 at 3921.893 kb on + strand, within LRK54_RS17965 at 3921.893 kb on + strand, within LRK54_RS17965 at 3921.893 kb on + strand, within LRK54_RS17965 at 3921.893 kb on + strand, within LRK54_RS17965 at 3921.893 kb on + strand, within LRK54_RS17965 at 3921.893 kb on + strand, within LRK54_RS17965 at 3921.894 kb on - strand, within LRK54_RS17965 at 3921.894 kb on - strand, within LRK54_RS17965 at 3921.894 kb on - strand, within LRK54_RS17965 at 3921.894 kb on - strand, within LRK54_RS17965 at 3921.894 kb on - strand, within LRK54_RS17965 at 3921.894 kb on - strand, within LRK54_RS17965 at 3921.894 kb on - strand, within LRK54_RS17965 at 3921.894 kb on - strand, within LRK54_RS17965 at 3921.894 kb on - strand, within LRK54_RS17965 at 3921.894 kb on - strand, within LRK54_RS17965 at 3921.894 kb on - strand, within LRK54_RS17965 at 3921.894 kb on - strand, within LRK54_RS17965 at 3921.931 kb on + strand, within LRK54_RS17965 at 3921.931 kb on + strand, within LRK54_RS17965 at 3921.931 kb on + strand, within LRK54_RS17965 at 3921.931 kb on + strand, within LRK54_RS17965 at 3921.931 kb on + strand, within LRK54_RS17965 at 3921.932 kb on - strand, within LRK54_RS17965 at 3921.932 kb on - strand, within LRK54_RS17965 at 3921.932 kb on - strand, within LRK54_RS17965 at 3921.932 kb on - strand, within LRK54_RS17965 at 3921.932 kb on - strand, within LRK54_RS17965 at 3921.932 kb on - strand, within LRK54_RS17965 at 3922.091 kb on + strand, within LRK54_RS17965 at 3922.091 kb on + strand, within LRK54_RS17965 at 3922.091 kb on + strand, within LRK54_RS17965 at 3922.091 kb on + strand, within LRK54_RS17965 at 3922.092 kb on - strand, within LRK54_RS17965 at 3922.092 kb on - strand, within LRK54_RS17965 at 3922.092 kb on - strand, within LRK54_RS17965 at 3922.092 kb on - strand, within LRK54_RS17965 at 3922.092 kb on - strand, within LRK54_RS17965 at 3922.092 kb on - strand, within LRK54_RS17965 at 3922.197 kb on + strand, within LRK54_RS17965 at 3922.197 kb on + strand, within LRK54_RS17965 at 3922.197 kb on + strand, within LRK54_RS17965 at 3922.197 kb on + strand, within LRK54_RS17965 at 3922.197 kb on + strand, within LRK54_RS17965 at 3922.197 kb on + strand, within LRK54_RS17965 at 3922.197 kb on + strand, within LRK54_RS17965 at 3922.197 kb on + strand, within LRK54_RS17965 at 3922.197 kb on + strand, within LRK54_RS17965 at 3922.197 kb on + strand, within LRK54_RS17965 at 3922.197 kb on + strand, within LRK54_RS17965 at 3922.197 kb on + strand, within LRK54_RS17965 at 3922.197 kb on + strand, within LRK54_RS17965 at 3922.197 kb on + strand, within LRK54_RS17965 at 3922.198 kb on - strand, within LRK54_RS17965 at 3922.198 kb on - strand, within LRK54_RS17965 at 3922.198 kb on - strand, within LRK54_RS17965 at 3922.198 kb on - strand, within LRK54_RS17965 at 3922.198 kb on - strand, within LRK54_RS17965 at 3922.198 kb on - strand, within LRK54_RS17965 at 3922.198 kb on - strand, within LRK54_RS17965 at 3922.198 kb on - strand, within LRK54_RS17965 at 3922.198 kb on - strand, within LRK54_RS17965 at 3922.198 kb on - strand, within LRK54_RS17965 at 3922.198 kb on - strand, within LRK54_RS17965 at 3922.198 kb on - strand, within LRK54_RS17965 at 3922.198 kb on - strand, within LRK54_RS17965 at 3922.198 kb on - strand, within LRK54_RS17965 at 3922.198 kb on - strand, within LRK54_RS17965 at 3922.198 kb on - strand, within LRK54_RS17965 at 3922.198 kb on - strand, within LRK54_RS17965 at 3922.198 kb on - strand, within LRK54_RS17965 at 3922.198 kb on - strand, within LRK54_RS17965 at 3922.198 kb on - strand, within LRK54_RS17965 at 3922.198 kb on - strand, within LRK54_RS17965 at 3922.198 kb on - strand, within LRK54_RS17965 at 3922.198 kb on - strand, within LRK54_RS17965 at 3922.265 kb on + strand, within LRK54_RS17965 at 3922.265 kb on + strand, within LRK54_RS17965 at 3922.265 kb on + strand, within LRK54_RS17965 at 3922.265 kb on + strand, within LRK54_RS17965 at 3922.265 kb on + strand, within LRK54_RS17965 at 3922.265 kb on + strand, within LRK54_RS17965 at 3922.265 kb on + strand, within LRK54_RS17965 at 3922.266 kb on - strand, within LRK54_RS17965 at 3922.266 kb on - strand, within LRK54_RS17965 at 3922.266 kb on - strand, within LRK54_RS17965 at 3922.266 kb on - strand, within LRK54_RS17965 at 3922.266 kb on - strand, within LRK54_RS17965 at 3922.266 kb on - strand, within LRK54_RS17965 at 3922.325 kb on + strand at 3922.325 kb on + strand at 3922.325 kb on + strand at 3922.325 kb on + strand at 3922.325 kb on + strand at 3922.325 kb on + strand at 3922.326 kb on - strand at 3922.326 kb on - strand at 3922.326 kb on - strand at 3922.326 kb on - strand at 3922.326 kb on - strand at 3922.326 kb on - strand at 3922.326 kb on - strand at 3922.326 kb on - strand at 3922.326 kb on - strand at 3922.326 kb on - strand at 3922.326 kb on - strand at 3922.326 kb on - strand at 3922.326 kb on - strand at 3922.326 kb on - strand at 3922.326 kb on - strand at 3922.326 kb on - strand at 3922.326 kb on - strand at 3922.326 kb on - strand at 3922.326 kb on - strand at 3922.326 kb on - strand at 3922.400 kb on + strand at 3922.401 kb on + strand at 3922.401 kb on + strand at 3922.401 kb on + strand at 3922.401 kb on + strand at 3922.401 kb on + strand at 3922.401 kb on + strand at 3922.401 kb on + strand at 3922.401 kb on + strand at 3922.401 kb on + strand at 3922.401 kb on + strand at 3922.401 kb on + strand at 3922.401 kb on + strand at 3922.401 kb on + strand at 3922.401 kb on + strand at 3922.401 kb on + strand at 3922.401 kb on + strand at 3922.401 kb on + strand at 3922.401 kb on + strand at 3922.401 kb on + strand at 3922.401 kb on + strand at 3922.401 kb on + strand at 3922.401 kb on + strand at 3922.401 kb on + strand at 3922.401 kb on + strand at 3922.401 kb on + strand at 3922.401 kb on + strand at 3922.401 kb on + strand at 3922.401 kb on + strand at 3922.401 kb on + strand at 3922.401 kb on + strand at 3922.401 kb on + strand at 3922.401 kb on + strand at 3922.401 kb on + strand at 3922.401 kb on + strand at 3922.401 kb on + strand at 3922.401 kb on + strand at 3922.401 kb on + strand at 3922.401 kb on + strand at 3922.402 kb on - strand at 3922.402 kb on - strand at 3922.402 kb on - strand at 3922.402 kb on - strand at 3922.402 kb on - strand at 3922.402 kb on - strand at 3922.402 kb on - strand at 3922.402 kb on - strand at 3922.402 kb on - strand at 3922.402 kb on - strand at 3922.402 kb on - strand at 3922.402 kb on - strand at 3922.402 kb on - strand at 3922.402 kb on - strand at 3922.402 kb on - strand at 3922.402 kb on - strand at 3922.402 kb on - strand at 3922.402 kb on - strand at 3922.402 kb on - strand at 3922.402 kb on - strand at 3922.402 kb on - strand at 3922.402 kb on - strand at 3922.402 kb on - strand at 3922.402 kb on - strand at 3922.402 kb on - strand at 3922.402 kb on - strand at 3922.402 kb on - strand at 3922.402 kb on - strand at 3922.402 kb on - strand at 3922.402 kb on - strand at 3922.402 kb on - strand at 3922.402 kb on - strand at 3922.402 kb on - strand at 3922.402 kb on - strand at 3922.402 kb on - strand at 3922.402 kb on - strand at 3922.402 kb on - strand at 3922.402 kb on - strand at 3922.402 kb on - strand at 3922.402 kb on - strand at 3922.402 kb on - strand at 3922.402 kb on - strand at 3922.402 kb on - strand at 3922.402 kb on - strand at 3922.402 kb on - strand at 3922.403 kb on + strand at 3922.403 kb on + strand at 3922.403 kb on + strand at 3922.403 kb on + strand at 3922.403 kb on + strand at 3922.403 kb on + strand at 3922.403 kb on + strand at 3922.403 kb on + strand at 3922.403 kb on + strand at 3922.403 kb on + strand at 3922.403 kb on + strand at 3922.403 kb on + strand at 3922.403 kb on + strand at 3922.403 kb on + strand at 3922.403 kb on + strand at 3922.403 kb on + strand at 3922.404 kb on - strand at 3922.404 kb on - strand at 3922.404 kb on - strand at 3922.404 kb on - strand at 3922.404 kb on - strand at 3922.404 kb on - strand at 3922.404 kb on - strand at 3922.404 kb on - strand at 3922.404 kb on - strand at 3922.404 kb on - strand at 3922.404 kb on - strand at 3922.404 kb on - strand at 3922.404 kb on - strand at 3922.404 kb on - strand at 3922.404 kb on - strand at 3922.404 kb on - strand at 3922.404 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with lithium chloride 15.625 mM remove 3,921,658 + LRK54_RS17965 0.30 -1.8 3,921,658 + LRK54_RS17965 0.30 +0.7 3,921,658 + LRK54_RS17965 0.30 -0.7 3,921,658 + LRK54_RS17965 0.30 -0.8 3,921,658 + LRK54_RS17965 0.30 -1.8 3,921,658 + LRK54_RS17965 0.30 +0.1 3,921,659 - LRK54_RS17965 0.30 +0.7 3,921,659 - LRK54_RS17965 0.30 +1.4 3,921,763 + LRK54_RS17965 0.40 +0.7 3,921,763 + LRK54_RS17965 0.40 +2.0 3,921,763 + LRK54_RS17965 0.40 -0.3 3,921,763 + LRK54_RS17965 0.40 +0.7 3,921,764 - LRK54_RS17965 0.40 -0.5 3,921,893 + LRK54_RS17965 0.52 +0.8 3,921,893 + LRK54_RS17965 0.52 -1.2 3,921,893 + LRK54_RS17965 0.52 -0.1 3,921,893 + LRK54_RS17965 0.52 -1.6 3,921,893 + LRK54_RS17965 0.52 -0.0 3,921,893 + LRK54_RS17965 0.52 +0.8 3,921,893 + LRK54_RS17965 0.52 +2.0 3,921,893 + LRK54_RS17965 0.52 +0.1 3,921,893 + LRK54_RS17965 0.52 +2.3 3,921,894 - LRK54_RS17965 0.52 +0.1 3,921,894 - LRK54_RS17965 0.52 -1.3 3,921,894 - LRK54_RS17965 0.52 +3.3 3,921,894 - LRK54_RS17965 0.52 -0.5 3,921,894 - LRK54_RS17965 0.52 -0.8 3,921,894 - LRK54_RS17965 0.52 +0.6 3,921,894 - LRK54_RS17965 0.52 +2.0 3,921,894 - LRK54_RS17965 0.52 +1.3 3,921,894 - LRK54_RS17965 0.52 +1.3 3,921,894 - LRK54_RS17965 0.52 +0.4 3,921,894 - LRK54_RS17965 0.52 +0.6 3,921,894 - LRK54_RS17965 0.52 +0.7 3,921,931 + LRK54_RS17965 0.56 +1.0 3,921,931 + LRK54_RS17965 0.56 +0.3 3,921,931 + LRK54_RS17965 0.56 +1.0 3,921,931 + LRK54_RS17965 0.56 +0.1 3,921,931 + LRK54_RS17965 0.56 -0.0 3,921,932 - LRK54_RS17965 0.56 -1.3 3,921,932 - LRK54_RS17965 0.56 +1.2 3,921,932 - LRK54_RS17965 0.56 +0.2 3,921,932 - LRK54_RS17965 0.56 +1.0 3,921,932 - LRK54_RS17965 0.56 +3.1 3,921,932 - LRK54_RS17965 0.56 +2.2 3,922,091 + LRK54_RS17965 0.71 -0.0 3,922,091 + LRK54_RS17965 0.71 -0.3 3,922,091 + LRK54_RS17965 0.71 +1.7 3,922,091 + LRK54_RS17965 0.71 -0.0 3,922,092 - LRK54_RS17965 0.71 -0.5 3,922,092 - LRK54_RS17965 0.71 -0.7 3,922,092 - LRK54_RS17965 0.71 +2.0 3,922,092 - LRK54_RS17965 0.71 +0.7 3,922,092 - LRK54_RS17965 0.71 +0.5 3,922,092 - LRK54_RS17965 0.71 +1.4 3,922,197 + LRK54_RS17965 0.80 -2.0 3,922,197 + LRK54_RS17965 0.80 +0.5 3,922,197 + LRK54_RS17965 0.80 -0.0 3,922,197 + LRK54_RS17965 0.80 +0.4 3,922,197 + LRK54_RS17965 0.80 +0.8 3,922,197 + LRK54_RS17965 0.80 -3.3 3,922,197 + LRK54_RS17965 0.80 -0.3 3,922,197 + LRK54_RS17965 0.80 +0.6 3,922,197 + LRK54_RS17965 0.80 -0.8 3,922,197 + LRK54_RS17965 0.80 -1.2 3,922,197 + LRK54_RS17965 0.80 -1.0 3,922,197 + LRK54_RS17965 0.80 -0.7 3,922,197 + LRK54_RS17965 0.80 -1.0 3,922,197 + LRK54_RS17965 0.80 -1.3 3,922,198 - LRK54_RS17965 0.81 +1.5 3,922,198 - LRK54_RS17965 0.81 +1.0 3,922,198 - LRK54_RS17965 0.81 -0.0 3,922,198 - LRK54_RS17965 0.81 +1.3 3,922,198 - LRK54_RS17965 0.81 +1.1 3,922,198 - LRK54_RS17965 0.81 +1.0 3,922,198 - LRK54_RS17965 0.81 +3.0 3,922,198 - LRK54_RS17965 0.81 +2.1 3,922,198 - LRK54_RS17965 0.81 +0.2 3,922,198 - LRK54_RS17965 0.81 +2.7 3,922,198 - LRK54_RS17965 0.81 +2.3 3,922,198 - LRK54_RS17965 0.81 +1.0 3,922,198 - LRK54_RS17965 0.81 +1.9 3,922,198 - LRK54_RS17965 0.81 +0.1 3,922,198 - LRK54_RS17965 0.81 +1.5 3,922,198 - LRK54_RS17965 0.81 -0.3 3,922,198 - LRK54_RS17965 0.81 +0.9 3,922,198 - LRK54_RS17965 0.81 +1.3 3,922,198 - LRK54_RS17965 0.81 +0.1 3,922,198 - LRK54_RS17965 0.81 +0.4 3,922,198 - LRK54_RS17965 0.81 -0.3 3,922,198 - LRK54_RS17965 0.81 -0.2 3,922,198 - LRK54_RS17965 0.81 +1.3 3,922,265 + LRK54_RS17965 0.87 +0.8 3,922,265 + LRK54_RS17965 0.87 -2.2 3,922,265 + LRK54_RS17965 0.87 -0.3 3,922,265 + LRK54_RS17965 0.87 +0.3 3,922,265 + LRK54_RS17965 0.87 -0.6 3,922,265 + LRK54_RS17965 0.87 -1.5 3,922,265 + LRK54_RS17965 0.87 -0.0 3,922,266 - LRK54_RS17965 0.87 +0.4 3,922,266 - LRK54_RS17965 0.87 +0.1 3,922,266 - LRK54_RS17965 0.87 +1.8 3,922,266 - LRK54_RS17965 0.87 +1.6 3,922,266 - LRK54_RS17965 0.87 +3.3 3,922,266 - LRK54_RS17965 0.87 +1.6 3,922,325 + -2.8 3,922,325 + -0.7 3,922,325 + -1.9 3,922,325 + -2.4 3,922,325 + -0.0 3,922,325 + -1.3 3,922,326 - +1.7 3,922,326 - +0.5 3,922,326 - +1.4 3,922,326 - +2.2 3,922,326 - +1.3 3,922,326 - +1.9 3,922,326 - +0.1 3,922,326 - +2.9 3,922,326 - +2.7 3,922,326 - +3.2 3,922,326 - +2.9 3,922,326 - +1.8 3,922,326 - +0.5 3,922,326 - +1.9 3,922,326 - +1.7 3,922,326 - -1.8 3,922,326 - -0.6 3,922,326 - +0.2 3,922,326 - +1.0 3,922,326 - +1.4 3,922,400 + -0.3 3,922,401 + -0.0 3,922,401 + -0.3 3,922,401 + -0.3 3,922,401 + -1.0 3,922,401 + +0.2 3,922,401 + +0.4 3,922,401 + -0.9 3,922,401 + -0.7 3,922,401 + +2.0 3,922,401 + -1.0 3,922,401 + +0.4 3,922,401 + -2.4 3,922,401 + +0.4 3,922,401 + -0.7 3,922,401 + +0.2 3,922,401 + +0.7 3,922,401 + -0.0 3,922,401 + -0.9 3,922,401 + -3.2 3,922,401 + -0.6 3,922,401 + -2.2 3,922,401 + -0.1 3,922,401 + -0.6 3,922,401 + -0.3 3,922,401 + -1.8 3,922,401 + -0.0 3,922,401 + +1.7 3,922,401 + -2.8 3,922,401 + +0.4 3,922,401 + -1.2 3,922,401 + -1.1 3,922,401 + +0.2 3,922,401 + -2.2 3,922,401 + -1.5 3,922,401 + +1.0 3,922,401 + +1.0 3,922,401 + -4.1 3,922,401 + -1.0 3,922,402 - +2.4 3,922,402 - +1.4 3,922,402 - +3.3 3,922,402 - +2.5 3,922,402 - +3.0 3,922,402 - +2.9 3,922,402 - +3.2 3,922,402 - +2.4 3,922,402 - +2.6 3,922,402 - +1.8 3,922,402 - +2.8 3,922,402 - +2.6 3,922,402 - +0.4 3,922,402 - +2.4 3,922,402 - +2.3 3,922,402 - +3.5 3,922,402 - +2.8 3,922,402 - +2.2 3,922,402 - +1.2 3,922,402 - +2.1 3,922,402 - +3.2 3,922,402 - +2.0 3,922,402 - +2.0 3,922,402 - +3.8 3,922,402 - +2.5 3,922,402 - +2.9 3,922,402 - +2.6 3,922,402 - +3.3 3,922,402 - +1.5 3,922,402 - +2.1 3,922,402 - +2.8 3,922,402 - +2.4 3,922,402 - +2.2 3,922,402 - +2.7 3,922,402 - +2.6 3,922,402 - +1.3 3,922,402 - +2.0 3,922,402 - -0.3 3,922,402 - +1.1 3,922,402 - +1.2 3,922,402 - +2.1 3,922,402 - +2.4 3,922,402 - +1.2 3,922,402 - +2.1 3,922,402 - -0.0 3,922,403 + -0.0 3,922,403 + -0.7 3,922,403 + +0.4 3,922,403 + -0.6 3,922,403 + -0.3 3,922,403 + -1.3 3,922,403 + -0.0 3,922,403 + -0.0 3,922,403 + -1.0 3,922,403 + -0.3 3,922,403 + -2.4 3,922,403 + -0.0 3,922,403 + -0.6 3,922,403 + -0.3 3,922,403 + -0.5 3,922,403 + -1.6 3,922,404 - -0.8 3,922,404 - +0.4 3,922,404 - -1.5 3,922,404 - +0.5 3,922,404 - +0.6 3,922,404 - -0.5 3,922,404 - +1.4 3,922,404 - +0.4 3,922,404 - +0.4 3,922,404 - +1.8 3,922,404 - -0.1 3,922,404 - -0.1 3,922,404 - +0.9 3,922,404 - -0.0 3,922,404 - -0.0 3,922,404 - +2.0 3,922,404 - -2.9
Or see this region's nucleotide sequence