Experiment: PYE with Benzalkonium Chloride 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01217 and CCNA_01218 overlap by 82 nucleotides CCNA_01218 and CCNA_01219 overlap by 4 nucleotides CCNA_01219 and CCNA_01220 are separated by 68 nucleotides
CCNA_01217: CCNA_01217 - phosphatidylglycerophosphate synthase, at 1,343,532 to 1,344,659
_01217
CCNA_01218: CCNA_01218 - sphingosine kinase/diacylglycerol kinase-related protein, at 1,344,578 to 1,345,570
_01218
CCNA_01219: CCNA_01219 - putative cytosolic protein, at 1,345,567 to 1,346,448
_01219
CCNA_01220: CCNA_01220 - 7-keto-8-aminopelargonate synthetase, at 1,346,517 to 1,347,731
_01220
Position (kb)
1344
1345
1346 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1343.627 kb on + strand at 1343.638 kb on + strand at 1343.649 kb on + strand, within CCNA_01217 at 1343.688 kb on - strand, within CCNA_01217 at 1343.754 kb on + strand, within CCNA_01217 at 1343.754 kb on + strand, within CCNA_01217 at 1343.762 kb on - strand, within CCNA_01217 at 1343.807 kb on + strand, within CCNA_01217 at 1343.854 kb on + strand, within CCNA_01217 at 1343.901 kb on + strand, within CCNA_01217 at 1343.901 kb on + strand, within CCNA_01217 at 1343.901 kb on + strand, within CCNA_01217 at 1343.901 kb on + strand, within CCNA_01217 at 1343.901 kb on + strand, within CCNA_01217 at 1343.901 kb on + strand, within CCNA_01217 at 1343.901 kb on + strand, within CCNA_01217 at 1343.901 kb on + strand, within CCNA_01217 at 1343.901 kb on + strand, within CCNA_01217 at 1343.901 kb on + strand, within CCNA_01217 at 1343.901 kb on + strand, within CCNA_01217 at 1343.901 kb on + strand, within CCNA_01217 at 1343.901 kb on + strand, within CCNA_01217 at 1343.901 kb on + strand, within CCNA_01217 at 1343.901 kb on + strand, within CCNA_01217 at 1343.901 kb on + strand, within CCNA_01217 at 1343.901 kb on + strand, within CCNA_01217 at 1343.901 kb on + strand, within CCNA_01217 at 1343.901 kb on + strand, within CCNA_01217 at 1343.901 kb on + strand, within CCNA_01217 at 1343.901 kb on + strand, within CCNA_01217 at 1343.901 kb on + strand, within CCNA_01217 at 1343.901 kb on + strand, within CCNA_01217 at 1343.901 kb on + strand, within CCNA_01217 at 1343.909 kb on - strand, within CCNA_01217 at 1343.909 kb on - strand, within CCNA_01217 at 1343.909 kb on - strand, within CCNA_01217 at 1343.909 kb on - strand, within CCNA_01217 at 1343.909 kb on - strand, within CCNA_01217 at 1343.909 kb on - strand, within CCNA_01217 at 1343.909 kb on - strand, within CCNA_01217 at 1343.909 kb on - strand, within CCNA_01217 at 1343.937 kb on + strand, within CCNA_01217 at 1343.973 kb on + strand, within CCNA_01217 at 1343.973 kb on + strand, within CCNA_01217 at 1343.987 kb on + strand, within CCNA_01217 at 1343.987 kb on + strand, within CCNA_01217 at 1344.027 kb on + strand, within CCNA_01217 at 1344.062 kb on - strand, within CCNA_01217 at 1344.143 kb on + strand, within CCNA_01217 at 1344.156 kb on + strand, within CCNA_01217 at 1344.168 kb on + strand, within CCNA_01217 at 1344.168 kb on + strand, within CCNA_01217 at 1344.168 kb on + strand, within CCNA_01217 at 1344.173 kb on + strand, within CCNA_01217 at 1344.178 kb on + strand, within CCNA_01217 at 1344.178 kb on + strand, within CCNA_01217 at 1344.207 kb on + strand, within CCNA_01217 at 1344.229 kb on - strand, within CCNA_01217 at 1344.231 kb on + strand, within CCNA_01217 at 1344.241 kb on + strand, within CCNA_01217 at 1344.361 kb on + strand, within CCNA_01217 at 1344.397 kb on + strand, within CCNA_01217 at 1344.425 kb on + strand, within CCNA_01217 at 1344.488 kb on + strand, within CCNA_01217 at 1344.540 kb on + strand, within CCNA_01217 at 1344.542 kb on + strand, within CCNA_01217 at 1344.573 kb on + strand at 1344.573 kb on + strand at 1344.573 kb on + strand at 1344.581 kb on - strand at 1344.636 kb on + strand at 1344.664 kb on + strand at 1344.664 kb on + strand at 1344.672 kb on - strand at 1344.672 kb on - strand at 1344.691 kb on - strand, within CCNA_01218 at 1344.750 kb on - strand, within CCNA_01218 at 1344.800 kb on + strand, within CCNA_01218 at 1344.800 kb on + strand, within CCNA_01218 at 1344.800 kb on + strand, within CCNA_01218 at 1344.800 kb on + strand, within CCNA_01218 at 1344.808 kb on - strand, within CCNA_01218 at 1344.915 kb on + strand, within CCNA_01218 at 1345.056 kb on - strand, within CCNA_01218 at 1345.101 kb on + strand, within CCNA_01218 at 1345.109 kb on - strand, within CCNA_01218 at 1345.151 kb on + strand, within CCNA_01218 at 1345.216 kb on + strand, within CCNA_01218 at 1345.243 kb on - strand, within CCNA_01218 at 1345.255 kb on + strand, within CCNA_01218 at 1345.258 kb on - strand, within CCNA_01218 at 1345.348 kb on - strand, within CCNA_01218 at 1345.375 kb on - strand, within CCNA_01218 at 1345.381 kb on + strand, within CCNA_01218 at 1345.389 kb on - strand, within CCNA_01218 at 1345.446 kb on - strand, within CCNA_01218 at 1345.518 kb on - strand at 1345.540 kb on + strand at 1345.541 kb on + strand at 1345.587 kb on + strand at 1345.610 kb on - strand at 1345.869 kb on + strand, within CCNA_01219 at 1345.924 kb on + strand, within CCNA_01219 at 1346.190 kb on - strand, within CCNA_01219 at 1346.223 kb on + strand, within CCNA_01219 at 1346.223 kb on + strand, within CCNA_01219 at 1346.260 kb on + strand, within CCNA_01219 at 1346.280 kb on + strand, within CCNA_01219 at 1346.350 kb on - strand, within CCNA_01219 at 1346.412 kb on + strand at 1346.418 kb on + strand at 1346.418 kb on + strand at 1346.418 kb on + strand at 1346.418 kb on + strand at 1346.418 kb on + strand at 1346.426 kb on - strand at 1346.426 kb on - strand at 1346.473 kb on + strand at 1346.515 kb on + strand at 1346.530 kb on + strand at 1346.550 kb on + strand at 1346.558 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Benzalkonium Chloride 0.0001 mg/ml remove 1,343,627 + +0.1 1,343,638 + +1.9 1,343,649 + CCNA_01217 0.10 +2.5 1,343,688 - CCNA_01217 0.14 +0.2 1,343,754 + CCNA_01217 0.20 -0.3 1,343,754 + CCNA_01217 0.20 +0.9 1,343,762 - CCNA_01217 0.20 -0.7 1,343,807 + CCNA_01217 0.24 +0.9 1,343,854 + CCNA_01217 0.29 -0.7 1,343,901 + CCNA_01217 0.33 +1.5 1,343,901 + CCNA_01217 0.33 -1.7 1,343,901 + CCNA_01217 0.33 +2.5 1,343,901 + CCNA_01217 0.33 -0.9 1,343,901 + CCNA_01217 0.33 +0.5 1,343,901 + CCNA_01217 0.33 -0.8 1,343,901 + CCNA_01217 0.33 +0.1 1,343,901 + CCNA_01217 0.33 -1.8 1,343,901 + CCNA_01217 0.33 -0.3 1,343,901 + CCNA_01217 0.33 -1.2 1,343,901 + CCNA_01217 0.33 +0.9 1,343,901 + CCNA_01217 0.33 -0.9 1,343,901 + CCNA_01217 0.33 -0.8 1,343,901 + CCNA_01217 0.33 -0.1 1,343,901 + CCNA_01217 0.33 -0.1 1,343,901 + CCNA_01217 0.33 -0.1 1,343,901 + CCNA_01217 0.33 +0.5 1,343,901 + CCNA_01217 0.33 +0.3 1,343,901 + CCNA_01217 0.33 -0.3 1,343,901 + CCNA_01217 0.33 -0.7 1,343,901 + CCNA_01217 0.33 +0.4 1,343,901 + CCNA_01217 0.33 +0.5 1,343,901 + CCNA_01217 0.33 -1.3 1,343,901 + CCNA_01217 0.33 +0.2 1,343,909 - CCNA_01217 0.33 +2.5 1,343,909 - CCNA_01217 0.33 +0.9 1,343,909 - CCNA_01217 0.33 +0.5 1,343,909 - CCNA_01217 0.33 -2.1 1,343,909 - CCNA_01217 0.33 +0.9 1,343,909 - CCNA_01217 0.33 +0.5 1,343,909 - CCNA_01217 0.33 -0.3 1,343,909 - CCNA_01217 0.33 +1.5 1,343,937 + CCNA_01217 0.36 +0.9 1,343,973 + CCNA_01217 0.39 +1.5 1,343,973 + CCNA_01217 0.39 -0.3 1,343,987 + CCNA_01217 0.40 +0.5 1,343,987 + CCNA_01217 0.40 -0.1 1,344,027 + CCNA_01217 0.44 +1.2 1,344,062 - CCNA_01217 0.47 +0.9 1,344,143 + CCNA_01217 0.54 +1.5 1,344,156 + CCNA_01217 0.55 -1.1 1,344,168 + CCNA_01217 0.56 -0.3 1,344,168 + CCNA_01217 0.56 +0.1 1,344,168 + CCNA_01217 0.56 +1.2 1,344,173 + CCNA_01217 0.57 -0.7 1,344,178 + CCNA_01217 0.57 +0.3 1,344,178 + CCNA_01217 0.57 -0.8 1,344,207 + CCNA_01217 0.60 +0.5 1,344,229 - CCNA_01217 0.62 -0.1 1,344,231 + CCNA_01217 0.62 -0.8 1,344,241 + CCNA_01217 0.63 -0.9 1,344,361 + CCNA_01217 0.73 -0.7 1,344,397 + CCNA_01217 0.77 -1.3 1,344,425 + CCNA_01217 0.79 -1.1 1,344,488 + CCNA_01217 0.85 +0.9 1,344,540 + CCNA_01217 0.89 -1.6 1,344,542 + CCNA_01217 0.90 -0.5 1,344,573 + -0.5 1,344,573 + -0.3 1,344,573 + +0.2 1,344,581 - +0.5 1,344,636 + +1.1 1,344,664 + +0.6 1,344,664 + +0.7 1,344,672 - -0.5 1,344,672 - +0.4 1,344,691 - CCNA_01218 0.11 -0.7 1,344,750 - CCNA_01218 0.17 +1.5 1,344,800 + CCNA_01218 0.22 +0.3 1,344,800 + CCNA_01218 0.22 -1.8 1,344,800 + CCNA_01218 0.22 -0.1 1,344,800 + CCNA_01218 0.22 -1.6 1,344,808 - CCNA_01218 0.23 -0.8 1,344,915 + CCNA_01218 0.34 +0.2 1,345,056 - CCNA_01218 0.48 -0.3 1,345,101 + CCNA_01218 0.53 +1.5 1,345,109 - CCNA_01218 0.53 -0.3 1,345,151 + CCNA_01218 0.58 +2.5 1,345,216 + CCNA_01218 0.64 -0.7 1,345,243 - CCNA_01218 0.67 -0.5 1,345,255 + CCNA_01218 0.68 -0.7 1,345,258 - CCNA_01218 0.68 +0.2 1,345,348 - CCNA_01218 0.78 +0.5 1,345,375 - CCNA_01218 0.80 -0.3 1,345,381 + CCNA_01218 0.81 -0.5 1,345,389 - CCNA_01218 0.82 -1.1 1,345,446 - CCNA_01218 0.87 -0.1 1,345,518 - -0.1 1,345,540 + +0.4 1,345,541 + -0.1 1,345,587 + -0.9 1,345,610 - -1.3 1,345,869 + CCNA_01219 0.34 -0.3 1,345,924 + CCNA_01219 0.40 +1.9 1,346,190 - CCNA_01219 0.71 +0.5 1,346,223 + CCNA_01219 0.74 +1.7 1,346,223 + CCNA_01219 0.74 +0.1 1,346,260 + CCNA_01219 0.79 +0.2 1,346,280 + CCNA_01219 0.81 +0.9 1,346,350 - CCNA_01219 0.89 +0.9 1,346,412 + +1.8 1,346,418 + +1.8 1,346,418 + +0.7 1,346,418 + +1.7 1,346,418 + +0.7 1,346,418 + +0.1 1,346,426 - +0.5 1,346,426 - +0.5 1,346,473 + +0.0 1,346,515 + -0.8 1,346,530 + +0.5 1,346,550 + -0.7 1,346,558 - +0.7
Or see this region's nucleotide sequence