Experiment: PYE with Benzalkonium Chloride 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00173 and CCNA_00174 overlap by 14 nucleotides CCNA_00174 and CCNA_00175 are separated by 11 nucleotides CCNA_00175 and CCNA_00176 overlap by 86 nucleotides CCNA_00176 and CCNA_00177 overlap by 32 nucleotides
CCNA_00173: CCNA_00173 - type II secretion pathway protein E, at 188,347 to 189,822
_00173
CCNA_00174: CCNA_00174 - type II secretion pathway protein F, at 189,809 to 191,020
_00174
CCNA_00175: CCNA_00175 - type II secretion pathway protein G, at 191,032 to 191,484
_00175
CCNA_00176: CCNA_00176 - type II secretion pathway protein H, at 191,399 to 191,956
_00176
CCNA_00177: CCNA_00177 - type II secretion pathway protein I, at 191,925 to 192,308
_00177
Position (kb) 189
190
191
192 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 188.838 kb on + strand, within CCNA_00173 at 188.838 kb on + strand, within CCNA_00173 at 188.844 kb on + strand, within CCNA_00173 at 188.846 kb on - strand, within CCNA_00173 at 188.846 kb on - strand, within CCNA_00173 at 188.846 kb on - strand, within CCNA_00173 at 188.846 kb on - strand, within CCNA_00173 at 188.846 kb on - strand, within CCNA_00173 at 188.846 kb on - strand, within CCNA_00173 at 188.852 kb on - strand at 188.925 kb on + strand, within CCNA_00173 at 188.929 kb on - strand, within CCNA_00173 at 188.935 kb on + strand, within CCNA_00173 at 188.971 kb on - strand, within CCNA_00173 at 188.996 kb on + strand, within CCNA_00173 at 189.001 kb on + strand, within CCNA_00173 at 189.009 kb on - strand, within CCNA_00173 at 189.011 kb on + strand, within CCNA_00173 at 189.011 kb on - strand, within CCNA_00173 at 189.016 kb on - strand, within CCNA_00173 at 189.018 kb on + strand, within CCNA_00173 at 189.020 kb on - strand, within CCNA_00173 at 189.025 kb on - strand, within CCNA_00173 at 189.051 kb on + strand, within CCNA_00173 at 189.187 kb on + strand, within CCNA_00173 at 189.199 kb on + strand, within CCNA_00173 at 189.211 kb on + strand, within CCNA_00173 at 189.216 kb on + strand, within CCNA_00173 at 189.219 kb on - strand, within CCNA_00173 at 189.222 kb on + strand, within CCNA_00173 at 189.222 kb on + strand, within CCNA_00173 at 189.238 kb on + strand, within CCNA_00173 at 189.263 kb on + strand, within CCNA_00173 at 189.281 kb on + strand, within CCNA_00173 at 189.325 kb on + strand, within CCNA_00173 at 189.337 kb on + strand, within CCNA_00173 at 189.346 kb on + strand, within CCNA_00173 at 189.403 kb on + strand, within CCNA_00173 at 189.406 kb on - strand, within CCNA_00173 at 189.444 kb on + strand, within CCNA_00173 at 189.444 kb on + strand, within CCNA_00173 at 189.452 kb on - strand, within CCNA_00173 at 189.454 kb on - strand, within CCNA_00173 at 189.483 kb on - strand, within CCNA_00173 at 189.538 kb on + strand, within CCNA_00173 at 189.552 kb on + strand, within CCNA_00173 at 189.560 kb on - strand, within CCNA_00173 at 189.603 kb on + strand, within CCNA_00173 at 189.632 kb on + strand, within CCNA_00173 at 189.637 kb on + strand, within CCNA_00173 at 189.638 kb on - strand, within CCNA_00173 at 189.640 kb on - strand, within CCNA_00173 at 189.655 kb on + strand, within CCNA_00173 at 189.663 kb on - strand, within CCNA_00173 at 189.688 kb on + strand at 189.718 kb on - strand at 189.730 kb on - strand at 189.748 kb on + strand at 189.751 kb on - strand at 189.756 kb on - strand at 189.766 kb on - strand at 189.777 kb on - strand at 189.815 kb on + strand at 189.816 kb on + strand at 189.816 kb on + strand at 189.829 kb on + strand at 189.873 kb on - strand at 189.873 kb on - strand at 189.894 kb on - strand at 189.974 kb on + strand, within CCNA_00174 at 189.994 kb on - strand, within CCNA_00174 at 189.994 kb on - strand, within CCNA_00174 at 190.050 kb on + strand, within CCNA_00174 at 190.052 kb on + strand, within CCNA_00174 at 190.054 kb on + strand, within CCNA_00174 at 190.056 kb on + strand, within CCNA_00174 at 190.066 kb on + strand, within CCNA_00174 at 190.100 kb on - strand, within CCNA_00174 at 190.120 kb on + strand, within CCNA_00174 at 190.121 kb on - strand, within CCNA_00174 at 190.133 kb on + strand, within CCNA_00174 at 190.143 kb on - strand, within CCNA_00174 at 190.143 kb on - strand, within CCNA_00174 at 190.143 kb on - strand, within CCNA_00174 at 190.161 kb on - strand, within CCNA_00174 at 190.198 kb on - strand, within CCNA_00174 at 190.203 kb on - strand, within CCNA_00174 at 190.237 kb on + strand, within CCNA_00174 at 190.280 kb on + strand, within CCNA_00174 at 190.307 kb on + strand, within CCNA_00174 at 190.307 kb on + strand, within CCNA_00174 at 190.307 kb on + strand, within CCNA_00174 at 190.307 kb on + strand, within CCNA_00174 at 190.307 kb on + strand, within CCNA_00174 at 190.312 kb on + strand, within CCNA_00174 at 190.315 kb on - strand, within CCNA_00174 at 190.315 kb on - strand, within CCNA_00174 at 190.315 kb on - strand, within CCNA_00174 at 190.315 kb on - strand, within CCNA_00174 at 190.315 kb on - strand at 190.315 kb on - strand, within CCNA_00174 at 190.320 kb on - strand, within CCNA_00174 at 190.378 kb on + strand, within CCNA_00174 at 190.385 kb on - strand, within CCNA_00174 at 190.409 kb on + strand, within CCNA_00174 at 190.417 kb on - strand, within CCNA_00174 at 190.424 kb on + strand, within CCNA_00174 at 190.432 kb on - strand, within CCNA_00174 at 190.450 kb on + strand, within CCNA_00174 at 190.457 kb on + strand, within CCNA_00174 at 190.505 kb on + strand, within CCNA_00174 at 190.540 kb on + strand, within CCNA_00174 at 190.603 kb on + strand, within CCNA_00174 at 190.624 kb on - strand, within CCNA_00174 at 190.624 kb on - strand, within CCNA_00174 at 190.631 kb on - strand, within CCNA_00174 at 190.661 kb on + strand, within CCNA_00174 at 190.661 kb on + strand, within CCNA_00174 at 190.669 kb on - strand, within CCNA_00174 at 190.678 kb on - strand, within CCNA_00174 at 190.684 kb on - strand, within CCNA_00174 at 190.694 kb on + strand, within CCNA_00174 at 190.696 kb on + strand, within CCNA_00174 at 190.696 kb on + strand, within CCNA_00174 at 190.696 kb on + strand, within CCNA_00174 at 190.696 kb on + strand, within CCNA_00174 at 190.696 kb on + strand, within CCNA_00174 at 190.696 kb on + strand, within CCNA_00174 at 190.704 kb on - strand, within CCNA_00174 at 190.704 kb on - strand, within CCNA_00174 at 190.708 kb on + strand, within CCNA_00174 at 190.709 kb on - strand, within CCNA_00174 at 190.709 kb on - strand, within CCNA_00174 at 190.716 kb on - strand, within CCNA_00174 at 190.716 kb on - strand, within CCNA_00174 at 190.762 kb on + strand, within CCNA_00174 at 190.762 kb on + strand, within CCNA_00174 at 190.762 kb on + strand, within CCNA_00174 at 190.837 kb on - strand, within CCNA_00174 at 190.840 kb on + strand, within CCNA_00174 at 190.848 kb on - strand, within CCNA_00174 at 190.850 kb on + strand, within CCNA_00174 at 190.858 kb on - strand, within CCNA_00174 at 190.858 kb on - strand, within CCNA_00174 at 190.915 kb on - strand at 190.915 kb on - strand at 191.009 kb on + strand at 191.022 kb on - strand at 191.037 kb on - strand at 191.081 kb on + strand, within CCNA_00175 at 191.081 kb on + strand, within CCNA_00175 at 191.089 kb on - strand, within CCNA_00175 at 191.089 kb on - strand, within CCNA_00175 at 191.096 kb on + strand, within CCNA_00175 at 191.097 kb on + strand, within CCNA_00175 at 191.099 kb on - strand, within CCNA_00175 at 191.220 kb on - strand, within CCNA_00175 at 191.229 kb on - strand, within CCNA_00175 at 191.268 kb on + strand, within CCNA_00175 at 191.300 kb on + strand, within CCNA_00175 at 191.300 kb on + strand, within CCNA_00175 at 191.307 kb on - strand, within CCNA_00175 at 191.312 kb on - strand, within CCNA_00175 at 191.312 kb on - strand, within CCNA_00175 at 191.357 kb on - strand, within CCNA_00175 at 191.357 kb on - strand, within CCNA_00175 at 191.357 kb on - strand, within CCNA_00175 at 191.357 kb on - strand, within CCNA_00175 at 191.410 kb on - strand at 191.434 kb on - strand at 191.452 kb on + strand at 191.493 kb on - strand, within CCNA_00176 at 191.493 kb on - strand, within CCNA_00176 at 191.494 kb on + strand, within CCNA_00176 at 191.502 kb on - strand, within CCNA_00176 at 191.513 kb on + strand, within CCNA_00176 at 191.518 kb on + strand, within CCNA_00176 at 191.523 kb on + strand, within CCNA_00176 at 191.523 kb on + strand, within CCNA_00176 at 191.523 kb on + strand, within CCNA_00176 at 191.531 kb on - strand, within CCNA_00176 at 191.531 kb on - strand, within CCNA_00176 at 191.531 kb on - strand, within CCNA_00176 at 191.533 kb on + strand, within CCNA_00176 at 191.539 kb on + strand, within CCNA_00176 at 191.547 kb on - strand, within CCNA_00176 at 191.572 kb on + strand, within CCNA_00176 at 191.584 kb on + strand, within CCNA_00176 at 191.585 kb on + strand, within CCNA_00176 at 191.594 kb on - strand, within CCNA_00176 at 191.594 kb on - strand, within CCNA_00176 at 191.618 kb on + strand, within CCNA_00176 at 191.672 kb on + strand, within CCNA_00176 at 191.677 kb on + strand, within CCNA_00176 at 191.703 kb on - strand, within CCNA_00176 at 191.753 kb on + strand, within CCNA_00176 at 191.761 kb on - strand, within CCNA_00176 at 191.788 kb on + strand, within CCNA_00176 at 191.796 kb on - strand, within CCNA_00176 at 191.807 kb on - strand, within CCNA_00176 at 191.843 kb on - strand, within CCNA_00176 at 191.849 kb on + strand, within CCNA_00176 at 191.857 kb on - strand, within CCNA_00176 at 191.882 kb on - strand, within CCNA_00176 at 191.919 kb on + strand at 191.962 kb on + strand at 192.018 kb on + strand, within CCNA_00177
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Benzalkonium Chloride 0.0001 mg/ml remove 188,838 + CCNA_00173 0.33 +2.2 188,838 + CCNA_00173 0.33 -1.4 188,844 + CCNA_00173 0.34 -0.7 188,846 - CCNA_00173 0.34 +0.6 188,846 - CCNA_00173 0.34 -0.4 188,846 - CCNA_00173 0.34 -2.4 188,846 - CCNA_00173 0.34 +0.2 188,846 - CCNA_00173 0.34 -2.1 188,846 - CCNA_00173 0.34 +0.9 188,852 - +2.2 188,925 + CCNA_00173 0.39 +1.0 188,929 - CCNA_00173 0.39 +0.6 188,935 + CCNA_00173 0.40 -0.4 188,971 - CCNA_00173 0.42 -1.4 188,996 + CCNA_00173 0.44 -1.3 189,001 + CCNA_00173 0.44 -2.8 189,009 - CCNA_00173 0.45 -0.4 189,011 + CCNA_00173 0.45 -0.1 189,011 - CCNA_00173 0.45 -0.3 189,016 - CCNA_00173 0.45 -0.6 189,018 + CCNA_00173 0.45 +0.0 189,020 - CCNA_00173 0.46 -0.8 189,025 - CCNA_00173 0.46 -0.4 189,051 + CCNA_00173 0.48 +0.2 189,187 + CCNA_00173 0.57 -0.4 189,199 + CCNA_00173 0.58 -0.5 189,211 + CCNA_00173 0.59 +0.4 189,216 + CCNA_00173 0.59 -1.4 189,219 - CCNA_00173 0.59 -0.7 189,222 + CCNA_00173 0.59 -0.1 189,222 + CCNA_00173 0.59 -1.0 189,238 + CCNA_00173 0.60 +0.2 189,263 + CCNA_00173 0.62 +0.2 189,281 + CCNA_00173 0.63 +2.2 189,325 + CCNA_00173 0.66 +2.2 189,337 + CCNA_00173 0.67 -0.3 189,346 + CCNA_00173 0.68 -0.3 189,403 + CCNA_00173 0.72 -0.8 189,406 - CCNA_00173 0.72 -2.5 189,444 + CCNA_00173 0.74 -0.6 189,444 + CCNA_00173 0.74 -1.5 189,452 - CCNA_00173 0.75 -0.6 189,454 - CCNA_00173 0.75 -1.4 189,483 - CCNA_00173 0.77 -1.3 189,538 + CCNA_00173 0.81 -0.1 189,552 + CCNA_00173 0.82 +1.2 189,560 - CCNA_00173 0.82 -1.5 189,603 + CCNA_00173 0.85 +1.2 189,632 + CCNA_00173 0.87 +1.2 189,637 + CCNA_00173 0.87 -1.3 189,638 - CCNA_00173 0.87 -1.3 189,640 - CCNA_00173 0.88 -1.6 189,655 + CCNA_00173 0.89 +0.2 189,663 - CCNA_00173 0.89 +1.2 189,688 + +2.2 189,718 - -0.1 189,730 - +0.6 189,748 + -0.8 189,751 - +0.8 189,756 - -0.4 189,766 - -2.3 189,777 - -0.1 189,815 + -0.4 189,816 + -1.4 189,816 + +0.7 189,829 + +0.6 189,873 - -0.4 189,873 - -1.6 189,894 - +1.2 189,974 + CCNA_00174 0.14 +1.0 189,994 - CCNA_00174 0.15 -1.5 189,994 - CCNA_00174 0.15 -0.9 190,050 + CCNA_00174 0.20 +0.1 190,052 + CCNA_00174 0.20 -2.2 190,054 + CCNA_00174 0.20 +0.3 190,056 + CCNA_00174 0.20 -0.1 190,066 + CCNA_00174 0.21 +0.0 190,100 - CCNA_00174 0.24 -0.5 190,120 + CCNA_00174 0.26 +0.2 190,121 - CCNA_00174 0.26 -0.8 190,133 + CCNA_00174 0.27 -1.5 190,143 - CCNA_00174 0.28 -1.1 190,143 - CCNA_00174 0.28 -0.8 190,143 - CCNA_00174 0.28 -1.3 190,161 - CCNA_00174 0.29 -1.6 190,198 - CCNA_00174 0.32 -1.3 190,203 - CCNA_00174 0.33 +1.2 190,237 + CCNA_00174 0.35 +0.6 190,280 + CCNA_00174 0.39 -1.6 190,307 + CCNA_00174 0.41 +0.2 190,307 + CCNA_00174 0.41 -0.4 190,307 + CCNA_00174 0.41 -1.4 190,307 + CCNA_00174 0.41 -0.4 190,307 + CCNA_00174 0.41 -1.9 190,312 + CCNA_00174 0.42 -0.8 190,315 - CCNA_00174 0.42 -0.8 190,315 - CCNA_00174 0.42 -0.9 190,315 - CCNA_00174 0.42 -1.5 190,315 - CCNA_00174 0.42 -1.9 190,315 - -0.4 190,315 - CCNA_00174 0.42 +3.2 190,320 - CCNA_00174 0.42 +1.9 190,378 + CCNA_00174 0.47 -0.1 190,385 - CCNA_00174 0.48 -0.6 190,409 + CCNA_00174 0.50 -0.9 190,417 - CCNA_00174 0.50 +0.2 190,424 + CCNA_00174 0.51 -1.0 190,432 - CCNA_00174 0.51 -1.3 190,450 + CCNA_00174 0.53 -1.6 190,457 + CCNA_00174 0.53 -1.1 190,505 + CCNA_00174 0.57 +0.2 190,540 + CCNA_00174 0.60 -1.1 190,603 + CCNA_00174 0.66 -0.6 190,624 - CCNA_00174 0.67 -1.0 190,624 - CCNA_00174 0.67 +0.2 190,631 - CCNA_00174 0.68 -0.4 190,661 + CCNA_00174 0.70 -3.3 190,661 + CCNA_00174 0.70 -1.4 190,669 - CCNA_00174 0.71 -1.8 190,678 - CCNA_00174 0.72 -0.6 190,684 - CCNA_00174 0.72 -0.8 190,694 + CCNA_00174 0.73 -1.4 190,696 + CCNA_00174 0.73 -1.0 190,696 + CCNA_00174 0.73 +1.0 190,696 + CCNA_00174 0.73 -0.6 190,696 + CCNA_00174 0.73 -1.6 190,696 + CCNA_00174 0.73 +0.6 190,696 + CCNA_00174 0.73 -2.2 190,704 - CCNA_00174 0.74 +0.6 190,704 - CCNA_00174 0.74 -1.7 190,708 + CCNA_00174 0.74 -2.1 190,709 - CCNA_00174 0.74 -0.8 190,709 - CCNA_00174 0.74 +0.5 190,716 - CCNA_00174 0.75 -2.1 190,716 - CCNA_00174 0.75 -0.4 190,762 + CCNA_00174 0.79 -1.0 190,762 + CCNA_00174 0.79 +0.2 190,762 + CCNA_00174 0.79 +1.2 190,837 - CCNA_00174 0.85 -2.7 190,840 + CCNA_00174 0.85 -2.2 190,848 - CCNA_00174 0.86 -2.5 190,850 + CCNA_00174 0.86 +0.2 190,858 - CCNA_00174 0.87 -1.3 190,858 - CCNA_00174 0.87 -0.6 190,915 - -0.8 190,915 - +0.0 191,009 + -2.2 191,022 - +1.2 191,037 - -0.4 191,081 + CCNA_00175 0.11 +0.2 191,081 + CCNA_00175 0.11 -0.7 191,089 - CCNA_00175 0.13 +0.2 191,089 - CCNA_00175 0.13 -1.0 191,096 + CCNA_00175 0.14 -1.0 191,097 + CCNA_00175 0.14 -0.4 191,099 - CCNA_00175 0.15 -1.0 191,220 - CCNA_00175 0.42 -1.1 191,229 - CCNA_00175 0.43 -1.0 191,268 + CCNA_00175 0.52 +1.6 191,300 + CCNA_00175 0.59 -0.4 191,300 + CCNA_00175 0.59 -1.0 191,307 - CCNA_00175 0.61 -1.4 191,312 - CCNA_00175 0.62 -0.4 191,312 - CCNA_00175 0.62 +1.0 191,357 - CCNA_00175 0.72 -1.1 191,357 - CCNA_00175 0.72 -1.0 191,357 - CCNA_00175 0.72 +0.1 191,357 - CCNA_00175 0.72 +0.6 191,410 - +0.2 191,434 - -0.1 191,452 + +0.1 191,493 - CCNA_00176 0.17 -1.0 191,493 - CCNA_00176 0.17 -1.0 191,494 + CCNA_00176 0.17 -0.9 191,502 - CCNA_00176 0.18 -2.3 191,513 + CCNA_00176 0.20 +0.9 191,518 + CCNA_00176 0.21 +0.5 191,523 + CCNA_00176 0.22 -1.0 191,523 + CCNA_00176 0.22 +0.6 191,523 + CCNA_00176 0.22 -0.4 191,531 - CCNA_00176 0.24 -2.0 191,531 - CCNA_00176 0.24 -0.1 191,531 - CCNA_00176 0.24 -1.9 191,533 + CCNA_00176 0.24 +0.3 191,539 + CCNA_00176 0.25 -0.6 191,547 - CCNA_00176 0.27 -0.6 191,572 + CCNA_00176 0.31 -2.3 191,584 + CCNA_00176 0.33 +2.2 191,585 + CCNA_00176 0.33 +1.2 191,594 - CCNA_00176 0.35 -0.1 191,594 - CCNA_00176 0.35 -0.1 191,618 + CCNA_00176 0.39 +0.6 191,672 + CCNA_00176 0.49 -0.7 191,677 + CCNA_00176 0.50 -1.0 191,703 - CCNA_00176 0.54 -0.4 191,753 + CCNA_00176 0.63 -0.5 191,761 - CCNA_00176 0.65 -0.7 191,788 + CCNA_00176 0.70 -1.8 191,796 - CCNA_00176 0.71 -0.4 191,807 - CCNA_00176 0.73 -1.5 191,843 - CCNA_00176 0.80 -1.4 191,849 + CCNA_00176 0.81 -1.3 191,857 - CCNA_00176 0.82 -1.0 191,882 - CCNA_00176 0.87 -0.1 191,919 + +0.2 191,962 + -1.0 192,018 + CCNA_00177 0.24 -0.4
Or see this region's nucleotide sequence