Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS33575 and AZOBR_RS33580 are separated by 398 nucleotides AZOBR_RS33580 and AZOBR_RS33585 overlap by 41 nucleotides
AZOBR_RS33575: AZOBR_RS33575 - putative Methyltransferase FkbM family, at 53,392 to 54,123
_RS33575
AZOBR_RS33580: AZOBR_RS33580 - hypothetical protein, at 54,522 to 54,923
_RS33580
AZOBR_RS33585: AZOBR_RS33585 - hypothetical protein, at 54,883 to 55,767
_RS33585
Position (kb) 53
54
55 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 52.428 kb on + strand at 52.428 kb on + strand at 52.428 kb on + strand at 52.428 kb on + strand at 52.429 kb on - strand at 52.429 kb on - strand at 52.479 kb on + strand at 52.488 kb on + strand at 52.488 kb on + strand at 52.488 kb on + strand at 52.489 kb on - strand at 52.489 kb on - strand at 52.489 kb on - strand at 52.855 kb on + strand at 53.102 kb on - strand at 53.130 kb on + strand at 53.130 kb on + strand at 53.130 kb on + strand at 53.397 kb on + strand at 53.944 kb on + strand, within AZOBR_RS33575 at 53.944 kb on + strand, within AZOBR_RS33575 at 54.015 kb on + strand, within AZOBR_RS33575 at 54.015 kb on + strand, within AZOBR_RS33575 at 54.015 kb on + strand, within AZOBR_RS33575 at 54.016 kb on - strand, within AZOBR_RS33575 at 54.069 kb on - strand at 54.222 kb on - strand at 54.285 kb on + strand at 54.285 kb on + strand at 54.286 kb on - strand at 54.291 kb on - strand at 54.442 kb on - strand at 54.457 kb on + strand at 54.458 kb on - strand at 54.458 kb on - strand at 54.512 kb on + strand at 54.512 kb on + strand at 54.595 kb on - strand, within AZOBR_RS33580 at 54.678 kb on + strand, within AZOBR_RS33580 at 54.678 kb on + strand, within AZOBR_RS33580 at 54.678 kb on + strand, within AZOBR_RS33580 at 54.678 kb on + strand, within AZOBR_RS33580 at 54.679 kb on - strand, within AZOBR_RS33580 at 54.679 kb on - strand, within AZOBR_RS33580 at 54.679 kb on - strand, within AZOBR_RS33580 at 55.112 kb on - strand, within AZOBR_RS33585 at 55.112 kb on - strand, within AZOBR_RS33585
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 52,428 + -3.7 52,428 + -1.6 52,428 + -1.5 52,428 + -0.3 52,429 - -0.7 52,429 - -0.8 52,479 + -3.2 52,488 + +0.2 52,488 + +0.6 52,488 + -0.3 52,489 - -2.8 52,489 - -2.2 52,489 - +0.6 52,855 + -3.3 53,102 - -0.6 53,130 + -3.9 53,130 + -1.5 53,130 + +1.8 53,397 + +2.3 53,944 + AZOBR_RS33575 0.75 -3.2 53,944 + AZOBR_RS33575 0.75 +0.6 54,015 + AZOBR_RS33575 0.85 -1.7 54,015 + AZOBR_RS33575 0.85 -2.8 54,015 + AZOBR_RS33575 0.85 -1.1 54,016 - AZOBR_RS33575 0.85 +0.0 54,069 - -0.2 54,222 - -0.6 54,285 + +1.0 54,285 + +0.9 54,286 - -1.6 54,291 - -1.0 54,442 - -0.8 54,457 + -1.6 54,458 - -0.9 54,458 - -0.3 54,512 + -0.9 54,512 + -0.2 54,595 - AZOBR_RS33580 0.18 +3.2 54,678 + AZOBR_RS33580 0.39 +1.0 54,678 + AZOBR_RS33580 0.39 -0.6 54,678 + AZOBR_RS33580 0.39 -4.5 54,678 + AZOBR_RS33580 0.39 +0.3 54,679 - AZOBR_RS33580 0.39 -1.1 54,679 - AZOBR_RS33580 0.39 -4.6 54,679 - AZOBR_RS33580 0.39 -0.1 55,112 - AZOBR_RS33585 0.26 -1.1 55,112 - AZOBR_RS33585 0.26 -2.1
Or see this region's nucleotide sequence