Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS31120 and AZOBR_RS31125 are separated by 70 nucleotides
AZOBR_RS31120: AZOBR_RS31120 - adenosylmethionine-8-amino-7-oxononanoate aminotransferase, at 345,819 to 347,123
_RS31120
AZOBR_RS31125: AZOBR_RS31125 - biotin synthase, at 347,194 to 348,117
_RS31125
Position (kb) 345
346
347
348 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4 at 344.929 kb on + strand at 344.929 kb on + strand at 345.163 kb on + strand at 345.280 kb on + strand at 345.281 kb on - strand at 345.383 kb on - strand at 346.137 kb on + strand, within AZOBR_RS31120 at 346.138 kb on - strand, within AZOBR_RS31120 at 346.248 kb on + strand, within AZOBR_RS31120 at 346.249 kb on - strand, within AZOBR_RS31120 at 346.249 kb on - strand, within AZOBR_RS31120 at 346.300 kb on - strand, within AZOBR_RS31120 at 346.344 kb on + strand, within AZOBR_RS31120 at 346.956 kb on + strand, within AZOBR_RS31120 at 347.035 kb on + strand at 347.035 kb on + strand at 347.036 kb on - strand at 347.037 kb on + strand at 347.038 kb on - strand at 347.038 kb on - strand at 347.038 kb on - strand at 347.038 kb on - strand at 347.038 kb on - strand at 347.085 kb on + strand at 347.383 kb on + strand, within AZOBR_RS31125 at 347.632 kb on + strand, within AZOBR_RS31125 at 347.632 kb on + strand, within AZOBR_RS31125 at 347.633 kb on - strand, within AZOBR_RS31125 at 347.633 kb on - strand, within AZOBR_RS31125 at 347.635 kb on + strand, within AZOBR_RS31125 at 347.636 kb on - strand, within AZOBR_RS31125 at 347.636 kb on - strand, within AZOBR_RS31125 at 347.636 kb on - strand, within AZOBR_RS31125 at 347.636 kb on - strand, within AZOBR_RS31125 at 347.636 kb on - strand, within AZOBR_RS31125 at 347.636 kb on - strand, within AZOBR_RS31125 at 347.668 kb on + strand, within AZOBR_RS31125 at 347.669 kb on - strand, within AZOBR_RS31125 at 347.669 kb on - strand, within AZOBR_RS31125 at 347.959 kb on + strand, within AZOBR_RS31125 at 348.043 kb on + strand at 348.044 kb on - strand at 348.044 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 344,929 + +0.6 344,929 + +0.7 345,163 + -4.8 345,280 + +0.2 345,281 - +0.3 345,383 - +3.2 346,137 + AZOBR_RS31120 0.24 +0.2 346,138 - AZOBR_RS31120 0.24 -3.2 346,248 + AZOBR_RS31120 0.33 -0.2 346,249 - AZOBR_RS31120 0.33 -2.7 346,249 - AZOBR_RS31120 0.33 -0.4 346,300 - AZOBR_RS31120 0.37 -1.2 346,344 + AZOBR_RS31120 0.40 +0.2 346,956 + AZOBR_RS31120 0.87 -0.7 347,035 + -0.4 347,035 + +1.2 347,036 - -0.6 347,037 + -0.3 347,038 - -1.4 347,038 - -1.3 347,038 - -2.2 347,038 - -0.5 347,038 - +1.1 347,085 + +4.2 347,383 + AZOBR_RS31125 0.20 -0.1 347,632 + AZOBR_RS31125 0.47 -2.4 347,632 + AZOBR_RS31125 0.47 +0.8 347,633 - AZOBR_RS31125 0.48 -0.1 347,633 - AZOBR_RS31125 0.48 -2.3 347,635 + AZOBR_RS31125 0.48 +0.6 347,636 - AZOBR_RS31125 0.48 +2.1 347,636 - AZOBR_RS31125 0.48 +1.0 347,636 - AZOBR_RS31125 0.48 -1.2 347,636 - AZOBR_RS31125 0.48 +0.0 347,636 - AZOBR_RS31125 0.48 +1.1 347,636 - AZOBR_RS31125 0.48 -2.3 347,668 + AZOBR_RS31125 0.51 -2.9 347,669 - AZOBR_RS31125 0.51 -1.5 347,669 - AZOBR_RS31125 0.51 -0.7 347,959 + AZOBR_RS31125 0.83 -2.2 348,043 + +0.3 348,044 - -0.6 348,044 - +0.4
Or see this region's nucleotide sequence