Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS30150 and AZOBR_RS30155 are separated by 171 nucleotides AZOBR_RS30155 and AZOBR_RS30160 are separated by 501 nucleotides
AZOBR_RS30150: AZOBR_RS30150 - pyruvate formate lyase-activating protein, at 103,122 to 103,889
_RS30150
AZOBR_RS30155: AZOBR_RS30155 - pyruvate kinase, at 104,061 to 105,494
_RS30155
AZOBR_RS30160: AZOBR_RS30160 - chemotaxis protein CheY, at 105,996 to 108,557
_RS30160
Position (kb) 104
105
106 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6 at 103.357 kb on + strand, within AZOBR_RS30150 at 103.357 kb on + strand, within AZOBR_RS30150 at 103.357 kb on + strand, within AZOBR_RS30150 at 103.358 kb on - strand, within AZOBR_RS30150 at 103.358 kb on - strand, within AZOBR_RS30150 at 103.444 kb on + strand, within AZOBR_RS30150 at 103.579 kb on + strand, within AZOBR_RS30150 at 103.580 kb on - strand, within AZOBR_RS30150 at 103.666 kb on + strand at 103.747 kb on + strand, within AZOBR_RS30150 at 103.747 kb on + strand, within AZOBR_RS30150 at 103.787 kb on - strand, within AZOBR_RS30150 at 103.787 kb on - strand at 103.787 kb on - strand, within AZOBR_RS30150 at 103.964 kb on + strand at 103.964 kb on + strand at 103.964 kb on + strand at 103.964 kb on + strand at 103.964 kb on + strand at 103.965 kb on - strand at 103.965 kb on - strand at 103.965 kb on - strand at 104.347 kb on + strand, within AZOBR_RS30155 at 104.348 kb on - strand, within AZOBR_RS30155 at 105.557 kb on + strand at 105.861 kb on + strand at 105.995 kb on + strand at 106.489 kb on - strand, within AZOBR_RS30160 at 106.489 kb on - strand, within AZOBR_RS30160 at 106.489 kb on - strand, within AZOBR_RS30160
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 103,357 + AZOBR_RS30150 0.31 -1.3 103,357 + AZOBR_RS30150 0.31 +1.0 103,357 + AZOBR_RS30150 0.31 -0.9 103,358 - AZOBR_RS30150 0.31 -0.7 103,358 - AZOBR_RS30150 0.31 +0.2 103,444 + AZOBR_RS30150 0.42 -0.7 103,579 + AZOBR_RS30150 0.60 -2.0 103,580 - AZOBR_RS30150 0.60 +0.1 103,666 + +1.4 103,747 + AZOBR_RS30150 0.81 +1.2 103,747 + AZOBR_RS30150 0.81 +1.2 103,787 - AZOBR_RS30150 0.87 +1.6 103,787 - +6.0 103,787 - AZOBR_RS30150 0.87 -2.2 103,964 + +0.7 103,964 + +0.8 103,964 + -0.8 103,964 + -0.1 103,964 + +0.9 103,965 - -0.2 103,965 - -2.6 103,965 - -0.3 104,347 + AZOBR_RS30155 0.20 +2.1 104,348 - AZOBR_RS30155 0.20 -0.3 105,557 + +0.4 105,861 + +0.4 105,995 + -0.3 106,489 - AZOBR_RS30160 0.19 -0.0 106,489 - AZOBR_RS30160 0.19 -0.6 106,489 - AZOBR_RS30160 0.19 -1.8
Or see this region's nucleotide sequence