Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS22170 and AZOBR_RS22175 overlap by 4 nucleotides AZOBR_RS22175 and AZOBR_RS22180 overlap by 4 nucleotides AZOBR_RS22180 and AZOBR_RS22185 are separated by 148 nucleotides AZOBR_RS22185 and AZOBR_RS22190 overlap by 4 nucleotides AZOBR_RS22190 and AZOBR_RS22195 are separated by 96 nucleotides
AZOBR_RS22170: AZOBR_RS22170 - hypothetical protein, at 1,748,062 to 1,748,901
_RS22170
AZOBR_RS22175: AZOBR_RS22175 - hypothetical protein, at 1,748,898 to 1,749,152
_RS22175
AZOBR_RS22180: AZOBR_RS22180 - hypothetical protein, at 1,749,149 to 1,749,484
_RS22180
AZOBR_RS22185: AZOBR_RS22185 - hypothetical protein, at 1,749,633 to 1,751,510
_RS22185
AZOBR_RS22190: AZOBR_RS22190 - hypothetical protein, at 1,751,507 to 1,752,232
_RS22190
AZOBR_RS22195: AZOBR_RS22195 - organic solvent tolerance protein, at 1,752,329 to 1,754,749
_RS22195
Position (kb) 1749
1750
1751
1752 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1748.677 kb on + strand, within AZOBR_RS22170 at 1748.677 kb on + strand, within AZOBR_RS22170 at 1748.678 kb on - strand, within AZOBR_RS22170 at 1748.678 kb on - strand, within AZOBR_RS22170 at 1748.764 kb on + strand, within AZOBR_RS22170 at 1748.764 kb on + strand, within AZOBR_RS22170 at 1748.765 kb on - strand, within AZOBR_RS22170 at 1748.765 kb on - strand, within AZOBR_RS22170 at 1748.765 kb on - strand, within AZOBR_RS22170 at 1749.449 kb on + strand, within AZOBR_RS22180 at 1749.449 kb on + strand, within AZOBR_RS22180 at 1749.449 kb on + strand, within AZOBR_RS22180 at 1749.449 kb on + strand, within AZOBR_RS22180 at 1749.450 kb on - strand, within AZOBR_RS22180 at 1749.450 kb on - strand, within AZOBR_RS22180 at 1749.482 kb on + strand at 1749.482 kb on + strand at 1749.483 kb on - strand at 1749.483 kb on - strand at 1750.404 kb on + strand, within AZOBR_RS22185 at 1750.503 kb on + strand, within AZOBR_RS22185 at 1750.503 kb on + strand, within AZOBR_RS22185 at 1750.504 kb on - strand, within AZOBR_RS22185 at 1750.749 kb on + strand, within AZOBR_RS22185 at 1750.750 kb on - strand, within AZOBR_RS22185 at 1750.798 kb on - strand, within AZOBR_RS22185 at 1750.932 kb on + strand, within AZOBR_RS22185 at 1750.933 kb on - strand, within AZOBR_RS22185 at 1751.307 kb on + strand, within AZOBR_RS22185 at 1751.508 kb on + strand at 1752.266 kb on - strand at 1752.266 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 1,748,677 + AZOBR_RS22170 0.73 -0.3 1,748,677 + AZOBR_RS22170 0.73 -0.8 1,748,678 - AZOBR_RS22170 0.73 +0.3 1,748,678 - AZOBR_RS22170 0.73 -2.9 1,748,764 + AZOBR_RS22170 0.84 +0.9 1,748,764 + AZOBR_RS22170 0.84 +0.1 1,748,765 - AZOBR_RS22170 0.84 +0.2 1,748,765 - AZOBR_RS22170 0.84 -1.9 1,748,765 - AZOBR_RS22170 0.84 -0.3 1,749,449 + AZOBR_RS22180 0.89 +0.2 1,749,449 + AZOBR_RS22180 0.89 -1.7 1,749,449 + AZOBR_RS22180 0.89 +1.1 1,749,449 + AZOBR_RS22180 0.89 -0.7 1,749,450 - AZOBR_RS22180 0.90 -0.2 1,749,450 - AZOBR_RS22180 0.90 -0.9 1,749,482 + -0.1 1,749,482 + +0.0 1,749,483 - -0.5 1,749,483 - -2.5 1,750,404 + AZOBR_RS22185 0.41 -2.1 1,750,503 + AZOBR_RS22185 0.46 +1.7 1,750,503 + AZOBR_RS22185 0.46 -0.7 1,750,504 - AZOBR_RS22185 0.46 -2.0 1,750,749 + AZOBR_RS22185 0.59 -1.1 1,750,750 - AZOBR_RS22185 0.59 +1.2 1,750,798 - AZOBR_RS22185 0.62 -1.9 1,750,932 + AZOBR_RS22185 0.69 -2.1 1,750,933 - AZOBR_RS22185 0.69 -0.2 1,751,307 + AZOBR_RS22185 0.89 -2.2 1,751,508 + +0.4 1,752,266 - -0.1 1,752,266 - +1.0
Or see this region's nucleotide sequence