Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS08150 and AZOBR_RS08155 are separated by 120 nucleotides AZOBR_RS08155 and AZOBR_RS08160 overlap by 4 nucleotides AZOBR_RS08160 and AZOBR_RS08165 are separated by 351 nucleotides AZOBR_RS08165 and AZOBR_RS08170 are separated by 24 nucleotides
AZOBR_RS08150: AZOBR_RS08150 - transcriptional regulator, at 1,716,400 to 1,716,867
_RS08150
AZOBR_RS08155: AZOBR_RS08155 - hypothetical protein, at 1,716,988 to 1,717,818
_RS08155
AZOBR_RS08160: AZOBR_RS08160 - sugar transporter, at 1,717,815 to 1,720,814
_RS08160
AZOBR_RS08165: AZOBR_RS08165 - hypothetical protein, at 1,721,166 to 1,721,663
_RS08165
AZOBR_RS08170: AZOBR_RS08170 - carbon-nitrogen family hydrolase, at 1,721,688 to 1,722,584
_RS08170
Position (kb)
1717
1718
1719
1720
1721 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1716.844 kb on + strand at 1716.867 kb on + strand at 1716.870 kb on + strand at 1716.870 kb on + strand at 1716.871 kb on - strand at 1716.871 kb on - strand at 1716.871 kb on - strand at 1716.962 kb on + strand at 1716.963 kb on - strand at 1717.078 kb on + strand, within AZOBR_RS08155 at 1717.079 kb on - strand, within AZOBR_RS08155 at 1717.079 kb on - strand, within AZOBR_RS08155 at 1717.322 kb on + strand, within AZOBR_RS08155 at 1717.322 kb on + strand, within AZOBR_RS08155 at 1717.598 kb on + strand, within AZOBR_RS08155 at 1717.599 kb on - strand, within AZOBR_RS08155 at 1717.599 kb on - strand, within AZOBR_RS08155 at 1718.068 kb on + strand at 1718.500 kb on + strand, within AZOBR_RS08160 at 1718.500 kb on + strand, within AZOBR_RS08160 at 1718.501 kb on - strand, within AZOBR_RS08160 at 1718.501 kb on - strand, within AZOBR_RS08160 at 1718.749 kb on + strand, within AZOBR_RS08160 at 1718.750 kb on - strand, within AZOBR_RS08160 at 1718.812 kb on - strand, within AZOBR_RS08160 at 1718.812 kb on - strand, within AZOBR_RS08160 at 1719.241 kb on + strand, within AZOBR_RS08160 at 1719.242 kb on - strand, within AZOBR_RS08160 at 1719.311 kb on - strand, within AZOBR_RS08160 at 1719.311 kb on - strand, within AZOBR_RS08160 at 1719.605 kb on - strand, within AZOBR_RS08160 at 1720.175 kb on - strand, within AZOBR_RS08160 at 1720.175 kb on - strand, within AZOBR_RS08160 at 1720.175 kb on - strand, within AZOBR_RS08160 at 1720.175 kb on - strand, within AZOBR_RS08160 at 1720.175 kb on - strand, within AZOBR_RS08160 at 1720.385 kb on - strand, within AZOBR_RS08160 at 1720.583 kb on - strand at 1720.682 kb on - strand at 1720.825 kb on - strand at 1720.911 kb on - strand at 1720.974 kb on + strand at 1720.974 kb on + strand at 1720.975 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 1,716,844 + +1.2 1,716,867 + -0.5 1,716,870 + +0.6 1,716,870 + -0.3 1,716,871 - +2.3 1,716,871 - -0.2 1,716,871 - +0.2 1,716,962 + -0.9 1,716,963 - -1.6 1,717,078 + AZOBR_RS08155 0.11 -2.6 1,717,079 - AZOBR_RS08155 0.11 -2.7 1,717,079 - AZOBR_RS08155 0.11 -0.8 1,717,322 + AZOBR_RS08155 0.40 +1.4 1,717,322 + AZOBR_RS08155 0.40 -0.7 1,717,598 + AZOBR_RS08155 0.73 -0.2 1,717,599 - AZOBR_RS08155 0.74 +2.0 1,717,599 - AZOBR_RS08155 0.74 -0.4 1,718,068 + -1.6 1,718,500 + AZOBR_RS08160 0.23 -0.7 1,718,500 + AZOBR_RS08160 0.23 +0.7 1,718,501 - AZOBR_RS08160 0.23 +0.2 1,718,501 - AZOBR_RS08160 0.23 -2.4 1,718,749 + AZOBR_RS08160 0.31 +0.5 1,718,750 - AZOBR_RS08160 0.31 -2.1 1,718,812 - AZOBR_RS08160 0.33 -1.4 1,718,812 - AZOBR_RS08160 0.33 +1.6 1,719,241 + AZOBR_RS08160 0.48 -4.2 1,719,242 - AZOBR_RS08160 0.48 -0.1 1,719,311 - AZOBR_RS08160 0.50 -0.2 1,719,311 - AZOBR_RS08160 0.50 +0.4 1,719,605 - AZOBR_RS08160 0.60 +0.0 1,720,175 - AZOBR_RS08160 0.79 +1.3 1,720,175 - AZOBR_RS08160 0.79 +2.3 1,720,175 - AZOBR_RS08160 0.79 -0.4 1,720,175 - AZOBR_RS08160 0.79 +3.0 1,720,175 - AZOBR_RS08160 0.79 -1.0 1,720,385 - AZOBR_RS08160 0.86 +0.2 1,720,583 - +0.8 1,720,682 - +0.5 1,720,825 - +1.1 1,720,911 - -0.9 1,720,974 + +1.6 1,720,974 + +1.3 1,720,975 - +0.5
Or see this region's nucleotide sequence