Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS02190 and AZOBR_RS02195 are separated by 189 nucleotides AZOBR_RS02195 and AZOBR_RS02200 overlap by 13 nucleotides AZOBR_RS02200 and AZOBR_RS02205 are separated by 144 nucleotides
AZOBR_RS02190: AZOBR_RS02190 - methyl-accepting chemotaxis protein, at 448,645 to 450,336
_RS02190
AZOBR_RS02195: AZOBR_RS02195 - coproporphyrinogen III oxidase, at 450,526 to 451,692
_RS02195
AZOBR_RS02200: AZOBR_RS02200 - nucleoside-triphosphate diphosphatase, at 451,680 to 452,282
_RS02200
AZOBR_RS02205: AZOBR_RS02205 - ribonuclease PH, at 452,427 to 453,146
_RS02205
Position (kb) 450
451
452 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 449.852 kb on + strand, within AZOBR_RS02190 at 449.852 kb on + strand, within AZOBR_RS02190 at 449.852 kb on + strand, within AZOBR_RS02190 at 449.852 kb on + strand, within AZOBR_RS02190 at 449.853 kb on - strand, within AZOBR_RS02190 at 449.853 kb on - strand, within AZOBR_RS02190 at 449.853 kb on - strand, within AZOBR_RS02190 at 449.853 kb on - strand, within AZOBR_RS02190 at 449.903 kb on + strand, within AZOBR_RS02190 at 449.904 kb on - strand, within AZOBR_RS02190 at 450.059 kb on + strand, within AZOBR_RS02190 at 450.059 kb on + strand, within AZOBR_RS02190 at 450.059 kb on + strand, within AZOBR_RS02190 at 450.060 kb on - strand, within AZOBR_RS02190 at 450.060 kb on - strand, within AZOBR_RS02190 at 450.242 kb on + strand at 450.243 kb on - strand at 450.446 kb on + strand at 450.458 kb on + strand at 450.460 kb on + strand at 450.461 kb on - strand at 450.900 kb on - strand, within AZOBR_RS02195 at 450.918 kb on - strand, within AZOBR_RS02195 at 450.918 kb on - strand, within AZOBR_RS02195 at 450.971 kb on + strand, within AZOBR_RS02195 at 451.017 kb on - strand, within AZOBR_RS02195 at 451.193 kb on + strand, within AZOBR_RS02195 at 451.194 kb on - strand, within AZOBR_RS02195 at 451.620 kb on - strand at 451.655 kb on + strand at 451.655 kb on + strand at 451.655 kb on + strand at 451.655 kb on + strand at 451.656 kb on - strand at 451.805 kb on - strand, within AZOBR_RS02200 at 452.008 kb on + strand, within AZOBR_RS02200 at 452.008 kb on + strand at 452.008 kb on + strand, within AZOBR_RS02200 at 452.008 kb on + strand, within AZOBR_RS02200 at 452.008 kb on + strand, within AZOBR_RS02200 at 452.009 kb on - strand, within AZOBR_RS02200 at 452.009 kb on - strand, within AZOBR_RS02200 at 452.428 kb on + strand at 452.428 kb on + strand at 452.428 kb on + strand at 452.429 kb on - strand at 452.429 kb on - strand at 452.647 kb on + strand, within AZOBR_RS02205 at 452.647 kb on + strand, within AZOBR_RS02205 at 452.647 kb on + strand, within AZOBR_RS02205 at 452.647 kb on + strand, within AZOBR_RS02205 at 452.647 kb on + strand, within AZOBR_RS02205 at 452.647 kb on + strand, within AZOBR_RS02205
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 449,852 + AZOBR_RS02190 0.71 +0.8 449,852 + AZOBR_RS02190 0.71 -0.1 449,852 + AZOBR_RS02190 0.71 -2.0 449,852 + AZOBR_RS02190 0.71 +0.3 449,853 - AZOBR_RS02190 0.71 +0.7 449,853 - AZOBR_RS02190 0.71 +0.1 449,853 - AZOBR_RS02190 0.71 +0.7 449,853 - AZOBR_RS02190 0.71 +1.2 449,903 + AZOBR_RS02190 0.74 -0.3 449,904 - AZOBR_RS02190 0.74 +3.1 450,059 + AZOBR_RS02190 0.84 +0.4 450,059 + AZOBR_RS02190 0.84 -3.6 450,059 + AZOBR_RS02190 0.84 -0.2 450,060 - AZOBR_RS02190 0.84 -2.3 450,060 - AZOBR_RS02190 0.84 -0.1 450,242 + +1.0 450,243 - +0.5 450,446 + +0.6 450,458 + -1.6 450,460 + -2.5 450,461 - +1.0 450,900 - AZOBR_RS02195 0.32 +0.4 450,918 - AZOBR_RS02195 0.34 +0.3 450,918 - AZOBR_RS02195 0.34 +0.8 450,971 + AZOBR_RS02195 0.38 -0.5 451,017 - AZOBR_RS02195 0.42 -0.1 451,193 + AZOBR_RS02195 0.57 +1.2 451,194 - AZOBR_RS02195 0.57 +0.9 451,620 - -0.1 451,655 + -0.9 451,655 + -0.7 451,655 + -1.1 451,655 + -0.3 451,656 - -1.1 451,805 - AZOBR_RS02200 0.21 +1.8 452,008 + AZOBR_RS02200 0.54 +0.4 452,008 + -0.0 452,008 + AZOBR_RS02200 0.54 +1.2 452,008 + AZOBR_RS02200 0.54 +1.0 452,008 + AZOBR_RS02200 0.54 -0.3 452,009 - AZOBR_RS02200 0.55 +3.1 452,009 - AZOBR_RS02200 0.55 +2.2 452,428 + +1.1 452,428 + +0.9 452,428 + -0.4 452,429 - +1.1 452,429 - -0.1 452,647 + AZOBR_RS02205 0.31 -2.2 452,647 + AZOBR_RS02205 0.31 -0.4 452,647 + AZOBR_RS02205 0.31 -0.4 452,647 + AZOBR_RS02205 0.31 -0.0 452,647 + AZOBR_RS02205 0.31 -0.0 452,647 + AZOBR_RS02205 0.31 +1.6
Or see this region's nucleotide sequence