Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS01985 and AZOBR_RS01990 overlap by 4 nucleotides AZOBR_RS01990 and AZOBR_RS01995 are separated by 48 nucleotides AZOBR_RS01995 and AZOBR_RS02000 are separated by 25 nucleotides
AZOBR_RS01985: AZOBR_RS01985 - glycosyl transferase group 1, at 407,455 to 408,726
_RS01985
AZOBR_RS01990: AZOBR_RS01990 - phosphoglycerate mutase, at 408,723 to 409,307
_RS01990
AZOBR_RS01995: AZOBR_RS01995 - exodeoxyribonuclease III, at 409,356 to 410,150
_RS01995
AZOBR_RS02000: AZOBR_RS02000 - 5-carboxymethyl-2-hydroxymuconate isomerase, at 410,176 to 410,859
_RS02000
Position (kb) 408
409
410 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 407.773 kb on + strand, within AZOBR_RS01985 at 407.773 kb on + strand, within AZOBR_RS01985 at 407.773 kb on + strand, within AZOBR_RS01985 at 407.774 kb on - strand, within AZOBR_RS01985 at 407.774 kb on - strand, within AZOBR_RS01985 at 407.774 kb on - strand, within AZOBR_RS01985 at 407.774 kb on - strand, within AZOBR_RS01985 at 407.774 kb on - strand, within AZOBR_RS01985 at 407.774 kb on - strand, within AZOBR_RS01985 at 408.098 kb on - strand, within AZOBR_RS01985 at 408.395 kb on - strand, within AZOBR_RS01985 at 408.439 kb on + strand, within AZOBR_RS01985 at 408.440 kb on - strand, within AZOBR_RS01985 at 408.440 kb on - strand, within AZOBR_RS01985 at 408.864 kb on + strand, within AZOBR_RS01990 at 409.170 kb on + strand, within AZOBR_RS01990 at 409.525 kb on + strand, within AZOBR_RS01995 at 409.837 kb on + strand at 409.837 kb on + strand, within AZOBR_RS01995 at 409.837 kb on + strand, within AZOBR_RS01995 at 409.838 kb on - strand, within AZOBR_RS01995 at 409.838 kb on - strand, within AZOBR_RS01995 at 409.963 kb on + strand, within AZOBR_RS01995 at 409.963 kb on + strand, within AZOBR_RS01995 at 409.964 kb on - strand, within AZOBR_RS01995 at 409.964 kb on - strand, within AZOBR_RS01995 at 409.964 kb on - strand, within AZOBR_RS01995 at 409.996 kb on + strand, within AZOBR_RS01995 at 409.996 kb on + strand, within AZOBR_RS01995 at 409.996 kb on + strand, within AZOBR_RS01995 at 409.997 kb on - strand, within AZOBR_RS01995 at 410.166 kb on - strand at 410.166 kb on - strand at 410.273 kb on + strand, within AZOBR_RS02000 at 410.274 kb on - strand, within AZOBR_RS02000 at 410.274 kb on - strand, within AZOBR_RS02000
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 407,773 + AZOBR_RS01985 0.25 -3.7 407,773 + AZOBR_RS01985 0.25 +0.2 407,773 + AZOBR_RS01985 0.25 +0.1 407,774 - AZOBR_RS01985 0.25 -1.1 407,774 - AZOBR_RS01985 0.25 +1.6 407,774 - AZOBR_RS01985 0.25 +0.7 407,774 - AZOBR_RS01985 0.25 +0.3 407,774 - AZOBR_RS01985 0.25 -0.9 407,774 - AZOBR_RS01985 0.25 +1.2 408,098 - AZOBR_RS01985 0.51 +1.2 408,395 - AZOBR_RS01985 0.74 +0.0 408,439 + AZOBR_RS01985 0.77 -0.3 408,440 - AZOBR_RS01985 0.77 -2.4 408,440 - AZOBR_RS01985 0.77 +0.5 408,864 + AZOBR_RS01990 0.24 +4.8 409,170 + AZOBR_RS01990 0.76 +0.3 409,525 + AZOBR_RS01995 0.21 -0.4 409,837 + +0.6 409,837 + AZOBR_RS01995 0.61 +1.9 409,837 + AZOBR_RS01995 0.61 -2.7 409,838 - AZOBR_RS01995 0.61 -0.8 409,838 - AZOBR_RS01995 0.61 +1.6 409,963 + AZOBR_RS01995 0.76 -0.6 409,963 + AZOBR_RS01995 0.76 -3.4 409,964 - AZOBR_RS01995 0.76 +1.2 409,964 - AZOBR_RS01995 0.76 -2.6 409,964 - AZOBR_RS01995 0.76 +0.2 409,996 + AZOBR_RS01995 0.81 +0.1 409,996 + AZOBR_RS01995 0.81 +0.8 409,996 + AZOBR_RS01995 0.81 +0.7 409,997 - AZOBR_RS01995 0.81 -0.3 410,166 - +0.4 410,166 - +0.6 410,273 + AZOBR_RS02000 0.14 +0.7 410,274 - AZOBR_RS02000 0.14 -0.9 410,274 - AZOBR_RS02000 0.14 -0.9
Or see this region's nucleotide sequence