Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS01565 and AZOBR_RS01570 are separated by 82 nucleotides AZOBR_RS01570 and AZOBR_RS01575 are separated by 164 nucleotides AZOBR_RS01575 and AZOBR_RS01580 are separated by 19 nucleotides AZOBR_RS01580 and AZOBR_RS01585 are separated by 71 nucleotides
AZOBR_RS01565: AZOBR_RS01565 - endonuclease III, at 321,932 to 322,579
_RS01565
AZOBR_RS01570: AZOBR_RS01570 - hypothetical protein, at 322,662 to 323,180
_RS01570
AZOBR_RS01575: AZOBR_RS01575 - 6-O-methylguanine DNA methyltransferase, at 323,345 to 324,169
_RS01575
AZOBR_RS01580: AZOBR_RS01580 - dihydrodipicolinate reductase, at 324,189 to 324,986
_RS01580
AZOBR_RS01585: AZOBR_RS01585 - zinc transporter, at 325,058 to 326,044
_RS01585
Position (kb) 323
324
325 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 322.357 kb on - strand, within AZOBR_RS01565 at 322.491 kb on + strand, within AZOBR_RS01565 at 322.503 kb on + strand, within AZOBR_RS01565 at 322.503 kb on + strand, within AZOBR_RS01565 at 322.503 kb on + strand, within AZOBR_RS01565 at 322.504 kb on - strand, within AZOBR_RS01565 at 322.504 kb on - strand, within AZOBR_RS01565 at 322.905 kb on + strand, within AZOBR_RS01570 at 322.906 kb on - strand, within AZOBR_RS01570 at 323.609 kb on + strand, within AZOBR_RS01575 at 323.610 kb on - strand, within AZOBR_RS01575 at 323.610 kb on - strand, within AZOBR_RS01575 at 323.610 kb on - strand, within AZOBR_RS01575 at 324.086 kb on + strand, within AZOBR_RS01575 at 324.086 kb on + strand, within AZOBR_RS01575 at 324.167 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 322,357 - AZOBR_RS01565 0.66 -1.2 322,491 + AZOBR_RS01565 0.86 -5.3 322,503 + AZOBR_RS01565 0.88 +0.6 322,503 + AZOBR_RS01565 0.88 -0.6 322,503 + AZOBR_RS01565 0.88 +0.3 322,504 - AZOBR_RS01565 0.88 -1.4 322,504 - AZOBR_RS01565 0.88 +1.0 322,905 + AZOBR_RS01570 0.47 +1.6 322,906 - AZOBR_RS01570 0.47 +1.0 323,609 + AZOBR_RS01575 0.32 -0.3 323,610 - AZOBR_RS01575 0.32 -1.4 323,610 - AZOBR_RS01575 0.32 +2.1 323,610 - AZOBR_RS01575 0.32 -1.4 324,086 + AZOBR_RS01575 0.90 +0.2 324,086 + AZOBR_RS01575 0.90 -2.7 324,167 + +1.4
Or see this region's nucleotide sequence