Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS01325 and AZOBR_RS01330 are separated by 230 nucleotides AZOBR_RS01330 and AZOBR_RS01335 are separated by 84 nucleotides AZOBR_RS01335 and AZOBR_RS01340 overlap by 4 nucleotides
AZOBR_RS01325: AZOBR_RS01325 - 2-hydroxyacid dehydrogenase, at 276,474 to 277,463
_RS01325
AZOBR_RS01330: AZOBR_RS01330 - hypothetical protein, at 277,694 to 278,248
_RS01330
AZOBR_RS01335: AZOBR_RS01335 - hypothetical protein, at 278,333 to 278,926
_RS01335
AZOBR_RS01340: AZOBR_RS01340 - rubrerythrin, at 278,923 to 279,342
_RS01340
Position (kb) 277
278
279 Strain fitness (log2 ratio) -1
0
1 at 276.799 kb on + strand, within AZOBR_RS01325 at 276.799 kb on + strand, within AZOBR_RS01325 at 276.874 kb on + strand, within AZOBR_RS01325 at 276.874 kb on + strand, within AZOBR_RS01325 at 276.928 kb on + strand, within AZOBR_RS01325 at 277.463 kb on + strand at 277.463 kb on + strand at 277.643 kb on + strand at 277.643 kb on + strand at 277.645 kb on + strand at 277.645 kb on + strand at 278.225 kb on + strand at 278.225 kb on + strand at 278.395 kb on - strand, within AZOBR_RS01335 at 278.688 kb on + strand, within AZOBR_RS01335 at 278.688 kb on + strand, within AZOBR_RS01335 at 278.772 kb on + strand, within AZOBR_RS01335 at 278.871 kb on + strand at 279.053 kb on + strand, within AZOBR_RS01340 at 279.065 kb on + strand, within AZOBR_RS01340 at 279.065 kb on + strand, within AZOBR_RS01340 at 279.065 kb on + strand, within AZOBR_RS01340
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 276,799 + AZOBR_RS01325 0.33 +0.9 276,799 + AZOBR_RS01325 0.33 -0.7 276,874 + AZOBR_RS01325 0.40 -1.1 276,874 + AZOBR_RS01325 0.40 +0.7 276,928 + AZOBR_RS01325 0.46 +0.6 277,463 + +0.2 277,463 + +0.3 277,643 + +1.2 277,643 + +0.9 277,645 + +0.6 277,645 + -0.6 278,225 + +0.2 278,225 + -0.5 278,395 - AZOBR_RS01335 0.10 -1.5 278,688 + AZOBR_RS01335 0.60 +0.2 278,688 + AZOBR_RS01335 0.60 +0.1 278,772 + AZOBR_RS01335 0.74 +0.7 278,871 + +1.3 279,053 + AZOBR_RS01340 0.31 -0.9 279,065 + AZOBR_RS01340 0.34 -0.1 279,065 + AZOBR_RS01340 0.34 -0.1 279,065 + AZOBR_RS01340 0.34 +1.6
Or see this region's nucleotide sequence