Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS00780 and AZOBR_RS00785 are separated by 87 nucleotides AZOBR_RS00785 and AZOBR_RS00790 are separated by 280 nucleotides AZOBR_RS00790 and AZOBR_RS00795 overlap by 1 nucleotides AZOBR_RS00795 and AZOBR_RS00800 are separated by 27 nucleotides
AZOBR_RS00780: AZOBR_RS00780 - putative methyl-accepting chemotaxis protein, at 151,915 to 153,585
_RS00780
AZOBR_RS00785: AZOBR_RS00785 - argininosuccinate synthase, at 153,673 to 154,908
_RS00785
AZOBR_RS00790: AZOBR_RS00790 - hypothetical protein, at 155,189 to 155,500
_RS00790
AZOBR_RS00795: AZOBR_RS00795 - hypothetical protein, at 155,500 to 155,736
_RS00795
AZOBR_RS00800: AZOBR_RS00800 - 50S rRNA methyltransferase, at 155,764 to 156,933
_RS00800
Position (kb) 153
154
155 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 152.758 kb on + strand, within AZOBR_RS00780 at 152.758 kb on + strand, within AZOBR_RS00780 at 152.758 kb on + strand, within AZOBR_RS00780 at 152.758 kb on + strand, within AZOBR_RS00780 at 152.758 kb on + strand, within AZOBR_RS00780 at 152.758 kb on + strand, within AZOBR_RS00780 at 152.759 kb on - strand, within AZOBR_RS00780 at 152.759 kb on - strand, within AZOBR_RS00780 at 152.759 kb on - strand, within AZOBR_RS00780 at 152.759 kb on - strand, within AZOBR_RS00780 at 152.759 kb on - strand, within AZOBR_RS00780 at 152.759 kb on - strand, within AZOBR_RS00780 at 152.759 kb on - strand, within AZOBR_RS00780 at 154.990 kb on + strand at 154.990 kb on + strand at 154.990 kb on + strand at 154.991 kb on - strand at 154.991 kb on - strand at 154.991 kb on - strand at 154.991 kb on - strand at 154.991 kb on - strand at 154.991 kb on - strand at 154.995 kb on - strand at 154.995 kb on - strand at 155.271 kb on + strand, within AZOBR_RS00790 at 155.272 kb on - strand, within AZOBR_RS00790 at 155.272 kb on - strand, within AZOBR_RS00790 at 155.272 kb on - strand, within AZOBR_RS00790 at 155.272 kb on - strand, within AZOBR_RS00790 at 155.272 kb on - strand, within AZOBR_RS00790 at 155.277 kb on + strand, within AZOBR_RS00790 at 155.277 kb on + strand, within AZOBR_RS00790 at 155.457 kb on + strand, within AZOBR_RS00790 at 155.458 kb on - strand, within AZOBR_RS00790 at 155.458 kb on - strand, within AZOBR_RS00790
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 152,758 + AZOBR_RS00780 0.50 +0.7 152,758 + AZOBR_RS00780 0.50 +0.2 152,758 + AZOBR_RS00780 0.50 +1.4 152,758 + AZOBR_RS00780 0.50 -1.8 152,758 + AZOBR_RS00780 0.50 -0.6 152,758 + AZOBR_RS00780 0.50 -1.8 152,759 - AZOBR_RS00780 0.51 -0.3 152,759 - AZOBR_RS00780 0.51 -1.2 152,759 - AZOBR_RS00780 0.51 -2.1 152,759 - AZOBR_RS00780 0.51 -1.5 152,759 - AZOBR_RS00780 0.51 -1.7 152,759 - AZOBR_RS00780 0.51 -1.0 152,759 - AZOBR_RS00780 0.51 -0.5 154,990 + +1.2 154,990 + -0.7 154,990 + -0.7 154,991 - -3.4 154,991 - -1.2 154,991 - +0.0 154,991 - -0.9 154,991 - +0.2 154,991 - -0.3 154,995 - -0.6 154,995 - +0.8 155,271 + AZOBR_RS00790 0.26 -0.2 155,272 - AZOBR_RS00790 0.27 -1.5 155,272 - AZOBR_RS00790 0.27 +0.9 155,272 - AZOBR_RS00790 0.27 +0.0 155,272 - AZOBR_RS00790 0.27 +1.4 155,272 - AZOBR_RS00790 0.27 -0.4 155,277 + AZOBR_RS00790 0.28 +0.2 155,277 + AZOBR_RS00790 0.28 +1.6 155,457 + AZOBR_RS00790 0.86 +1.1 155,458 - AZOBR_RS00790 0.86 +1.2 155,458 - AZOBR_RS00790 0.86 +0.1
Or see this region's nucleotide sequence