Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_30355 and AO356_30360 are separated by 63 nucleotides AO356_30360 and AO356_30365 are separated by 49 nucleotides AO356_30365 and AO356_30370 are separated by 68 nucleotides
AO356_30355: AO356_30355 - enoyl-CoA hydratase, at 7,085,525 to 7,086,319
_30355
AO356_30360: AO356_30360 - 3-hydroxypropionyl-CoA dehydratase, at 7,086,383 to 7,087,120
_30360
AO356_30365: AO356_30365 - CDP-6-deoxy-delta-3,4-glucoseen reductase, at 7,087,170 to 7,088,210
_30365
AO356_30370: AO356_30370 - CoA-binding protein, at 7,088,279 to 7,090,393
_30370
Position (kb) 7087
7088
7089 Strain fitness (log2 ratio) -1
0
1 at 7086.278 kb on + strand at 7086.279 kb on - strand at 7086.299 kb on - strand at 7086.322 kb on + strand at 7086.323 kb on - strand at 7086.323 kb on - strand at 7086.560 kb on + strand, within AO356_30360 at 7086.659 kb on + strand, within AO356_30360 at 7086.659 kb on + strand, within AO356_30360 at 7086.881 kb on - strand, within AO356_30360 at 7087.003 kb on + strand, within AO356_30360 at 7087.004 kb on - strand, within AO356_30360 at 7087.004 kb on - strand, within AO356_30360 at 7087.015 kb on + strand, within AO356_30360 at 7087.016 kb on - strand, within AO356_30360 at 7087.104 kb on - strand at 7087.104 kb on - strand at 7087.129 kb on - strand at 7087.129 kb on - strand at 7087.143 kb on - strand at 7087.172 kb on - strand at 7087.172 kb on - strand at 7087.292 kb on - strand, within AO356_30365 at 7087.365 kb on + strand, within AO356_30365 at 7087.366 kb on - strand, within AO356_30365 at 7087.366 kb on - strand, within AO356_30365 at 7087.398 kb on + strand, within AO356_30365 at 7087.399 kb on - strand, within AO356_30365 at 7087.399 kb on - strand, within AO356_30365 at 7087.399 kb on - strand, within AO356_30365 at 7087.451 kb on - strand, within AO356_30365 at 7087.481 kb on - strand, within AO356_30365 at 7087.488 kb on + strand, within AO356_30365 at 7087.488 kb on + strand, within AO356_30365 at 7087.488 kb on + strand, within AO356_30365 at 7087.684 kb on + strand, within AO356_30365 at 7087.752 kb on - strand, within AO356_30365 at 7087.777 kb on + strand, within AO356_30365 at 7087.777 kb on + strand, within AO356_30365 at 7087.778 kb on - strand, within AO356_30365 at 7087.778 kb on - strand, within AO356_30365 at 7087.889 kb on + strand, within AO356_30365 at 7087.890 kb on - strand, within AO356_30365 at 7088.121 kb on + strand at 7088.122 kb on - strand at 7088.122 kb on - strand at 7088.122 kb on - strand at 7088.240 kb on + strand at 7088.240 kb on + strand at 7088.240 kb on + strand at 7088.328 kb on - strand at 7088.328 kb on - strand at 7088.360 kb on - strand at 7088.550 kb on + strand, within AO356_30370 at 7088.550 kb on + strand, within AO356_30370 at 7088.570 kb on + strand, within AO356_30370 at 7088.571 kb on - strand, within AO356_30370 at 7088.760 kb on - strand, within AO356_30370 at 7088.805 kb on - strand, within AO356_30370 at 7088.952 kb on - strand, within AO356_30370
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 7,086,278 + -1.0 7,086,279 - +0.3 7,086,299 - -0.0 7,086,322 + -1.3 7,086,323 - +0.3 7,086,323 - -0.5 7,086,560 + AO356_30360 0.24 -0.4 7,086,659 + AO356_30360 0.37 -0.7 7,086,659 + AO356_30360 0.37 +0.0 7,086,881 - AO356_30360 0.67 -0.7 7,087,003 + AO356_30360 0.84 -0.0 7,087,004 - AO356_30360 0.84 +0.1 7,087,004 - AO356_30360 0.84 +0.1 7,087,015 + AO356_30360 0.86 -1.3 7,087,016 - AO356_30360 0.86 -0.7 7,087,104 - +1.7 7,087,104 - +0.8 7,087,129 - -1.2 7,087,129 - -0.6 7,087,143 - +0.7 7,087,172 - -1.2 7,087,172 - +0.1 7,087,292 - AO356_30365 0.12 +0.3 7,087,365 + AO356_30365 0.19 +0.4 7,087,366 - AO356_30365 0.19 +0.1 7,087,366 - AO356_30365 0.19 -0.4 7,087,398 + AO356_30365 0.22 -0.8 7,087,399 - AO356_30365 0.22 -0.1 7,087,399 - AO356_30365 0.22 -0.2 7,087,399 - AO356_30365 0.22 +0.3 7,087,451 - AO356_30365 0.27 -0.4 7,087,481 - AO356_30365 0.30 -0.5 7,087,488 + AO356_30365 0.31 +0.4 7,087,488 + AO356_30365 0.31 +1.1 7,087,488 + AO356_30365 0.31 -1.3 7,087,684 + AO356_30365 0.49 -0.6 7,087,752 - AO356_30365 0.56 +0.5 7,087,777 + AO356_30365 0.58 -0.4 7,087,777 + AO356_30365 0.58 +0.9 7,087,778 - AO356_30365 0.58 -1.0 7,087,778 - AO356_30365 0.58 +0.4 7,087,889 + AO356_30365 0.69 +0.6 7,087,890 - AO356_30365 0.69 +0.1 7,088,121 + -0.4 7,088,122 - +0.6 7,088,122 - +0.5 7,088,122 - -0.2 7,088,240 + +0.1 7,088,240 + -1.0 7,088,240 + +0.1 7,088,328 - -0.8 7,088,328 - -0.2 7,088,360 - +0.3 7,088,550 + AO356_30370 0.13 +0.6 7,088,550 + AO356_30370 0.13 +1.0 7,088,570 + AO356_30370 0.14 +0.7 7,088,571 - AO356_30370 0.14 +0.1 7,088,760 - AO356_30370 0.23 +0.9 7,088,805 - AO356_30370 0.25 -0.5 7,088,952 - AO356_30370 0.32 -0.7
Or see this region's nucleotide sequence