Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_22235 and AO356_22240 are separated by 2 nucleotides AO356_22240 and AO356_22245 are separated by 139 nucleotides AO356_22245 and AO356_22250 are separated by 129 nucleotides AO356_22250 and AO356_22255 are separated by 164 nucleotides
AO356_22235: AO356_22235 - DNA repair protein, at 4,974,891 to 4,975,226
_22235
AO356_22240: AO356_22240 - hypothetical protein, at 4,975,229 to 4,975,489
_22240
AO356_22245: AO356_22245 - hypothetical protein, at 4,975,629 to 4,975,979
_22245
AO356_22250: AO356_22250 - GCN5 family acetyltransferase, at 4,976,109 to 4,976,537
_22250
AO356_22255: AO356_22255 - gamma-glutamyltranspeptidase, at 4,976,702 to 4,978,429
_22255
Position (kb)
4976
4977 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4975.308 kb on + strand, within AO356_22240 at 4975.308 kb on + strand, within AO356_22240 at 4975.308 kb on + strand, within AO356_22240 at 4975.309 kb on - strand, within AO356_22240 at 4975.309 kb on - strand, within AO356_22240 at 4975.365 kb on + strand, within AO356_22240 at 4975.366 kb on - strand, within AO356_22240 at 4975.449 kb on + strand, within AO356_22240 at 4975.450 kb on - strand, within AO356_22240 at 4975.450 kb on - strand, within AO356_22240 at 4975.467 kb on + strand at 4975.468 kb on - strand at 4975.468 kb on - strand at 4975.468 kb on - strand at 4975.566 kb on + strand at 4975.597 kb on + strand at 4975.598 kb on - strand at 4975.598 kb on - strand at 4975.598 kb on - strand at 4975.716 kb on + strand, within AO356_22245 at 4975.716 kb on + strand, within AO356_22245 at 4975.717 kb on - strand, within AO356_22245 at 4975.815 kb on + strand, within AO356_22245 at 4975.816 kb on - strand, within AO356_22245 at 4975.914 kb on + strand, within AO356_22245 at 4975.914 kb on + strand, within AO356_22245 at 4975.915 kb on - strand, within AO356_22245 at 4975.915 kb on - strand, within AO356_22245 at 4975.915 kb on - strand, within AO356_22245 at 4975.915 kb on - strand, within AO356_22245 at 4975.977 kb on + strand at 4975.977 kb on + strand at 4975.977 kb on + strand at 4975.977 kb on + strand at 4975.978 kb on - strand at 4975.978 kb on - strand at 4975.978 kb on - strand at 4975.978 kb on - strand at 4976.007 kb on - strand at 4976.007 kb on - strand at 4976.007 kb on - strand at 4976.007 kb on - strand at 4976.066 kb on - strand at 4976.161 kb on + strand, within AO356_22250 at 4976.204 kb on - strand, within AO356_22250 at 4976.347 kb on + strand, within AO356_22250 at 4976.347 kb on + strand, within AO356_22250 at 4976.348 kb on - strand, within AO356_22250 at 4976.348 kb on - strand, within AO356_22250 at 4976.374 kb on + strand, within AO356_22250 at 4976.470 kb on - strand, within AO356_22250 at 4976.530 kb on - strand at 4976.629 kb on + strand at 4976.629 kb on + strand at 4976.673 kb on + strand at 4976.673 kb on + strand at 4976.900 kb on + strand, within AO356_22255 at 4977.035 kb on + strand, within AO356_22255 at 4977.035 kb on + strand, within AO356_22255 at 4977.035 kb on + strand, within AO356_22255 at 4977.035 kb on + strand, within AO356_22255 at 4977.035 kb on + strand, within AO356_22255 at 4977.035 kb on + strand, within AO356_22255 at 4977.035 kb on + strand, within AO356_22255 at 4977.035 kb on + strand, within AO356_22255 at 4977.036 kb on - strand, within AO356_22255 at 4977.036 kb on - strand, within AO356_22255 at 4977.036 kb on - strand, within AO356_22255 at 4977.036 kb on - strand, within AO356_22255 at 4977.140 kb on + strand, within AO356_22255 at 4977.141 kb on - strand, within AO356_22255 at 4977.141 kb on - strand, within AO356_22255 at 4977.386 kb on + strand, within AO356_22255 at 4977.387 kb on - strand, within AO356_22255 at 4977.387 kb on - strand, within AO356_22255 at 4977.467 kb on + strand, within AO356_22255 at 4977.468 kb on - strand, within AO356_22255 at 4977.468 kb on - strand, within AO356_22255 at 4977.468 kb on - strand, within AO356_22255 at 4977.468 kb on - strand, within AO356_22255 at 4977.468 kb on - strand, within AO356_22255 at 4977.468 kb on - strand, within AO356_22255 at 4977.513 kb on - strand, within AO356_22255 at 4977.520 kb on + strand, within AO356_22255 at 4977.520 kb on + strand, within AO356_22255 at 4977.520 kb on + strand, within AO356_22255 at 4977.521 kb on - strand, within AO356_22255 at 4977.521 kb on - strand, within AO356_22255
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 4,975,308 + AO356_22240 0.30 -0.2 4,975,308 + AO356_22240 0.30 -0.5 4,975,308 + AO356_22240 0.30 +0.4 4,975,309 - AO356_22240 0.31 +0.1 4,975,309 - AO356_22240 0.31 +0.3 4,975,365 + AO356_22240 0.52 -0.1 4,975,366 - AO356_22240 0.52 +1.2 4,975,449 + AO356_22240 0.84 +0.3 4,975,450 - AO356_22240 0.85 +1.3 4,975,450 - AO356_22240 0.85 +1.1 4,975,467 + +0.1 4,975,468 - +0.1 4,975,468 - +0.3 4,975,468 - +0.8 4,975,566 + -0.2 4,975,597 + -0.6 4,975,598 - +0.8 4,975,598 - -0.4 4,975,598 - -0.0 4,975,716 + AO356_22245 0.25 +1.2 4,975,716 + AO356_22245 0.25 -0.2 4,975,717 - AO356_22245 0.25 -0.8 4,975,815 + AO356_22245 0.53 +1.3 4,975,816 - AO356_22245 0.53 +0.5 4,975,914 + AO356_22245 0.81 +0.2 4,975,914 + AO356_22245 0.81 +1.0 4,975,915 - AO356_22245 0.81 +0.9 4,975,915 - AO356_22245 0.81 -0.3 4,975,915 - AO356_22245 0.81 +0.9 4,975,915 - AO356_22245 0.81 +0.2 4,975,977 + +0.1 4,975,977 + -0.7 4,975,977 + +0.3 4,975,977 + -0.8 4,975,978 - -0.9 4,975,978 - -0.7 4,975,978 - +0.1 4,975,978 - +0.1 4,976,007 - +0.2 4,976,007 - +0.5 4,976,007 - +0.2 4,976,007 - -0.1 4,976,066 - -0.6 4,976,161 + AO356_22250 0.12 -0.0 4,976,204 - AO356_22250 0.22 +1.8 4,976,347 + AO356_22250 0.55 -2.9 4,976,347 + AO356_22250 0.55 +0.2 4,976,348 - AO356_22250 0.56 -0.2 4,976,348 - AO356_22250 0.56 +0.4 4,976,374 + AO356_22250 0.62 +0.2 4,976,470 - AO356_22250 0.84 +1.4 4,976,530 - +0.9 4,976,629 + +0.2 4,976,629 + -1.6 4,976,673 + +0.8 4,976,673 + +0.0 4,976,900 + AO356_22255 0.11 -0.7 4,977,035 + AO356_22255 0.19 +0.9 4,977,035 + AO356_22255 0.19 -0.3 4,977,035 + AO356_22255 0.19 +0.3 4,977,035 + AO356_22255 0.19 +0.0 4,977,035 + AO356_22255 0.19 -0.2 4,977,035 + AO356_22255 0.19 -0.2 4,977,035 + AO356_22255 0.19 +0.9 4,977,035 + AO356_22255 0.19 +0.5 4,977,036 - AO356_22255 0.19 -0.9 4,977,036 - AO356_22255 0.19 -0.3 4,977,036 - AO356_22255 0.19 +0.4 4,977,036 - AO356_22255 0.19 +0.6 4,977,140 + AO356_22255 0.25 +0.4 4,977,141 - AO356_22255 0.25 +0.8 4,977,141 - AO356_22255 0.25 -0.5 4,977,386 + AO356_22255 0.40 -0.1 4,977,387 - AO356_22255 0.40 -0.0 4,977,387 - AO356_22255 0.40 -0.3 4,977,467 + AO356_22255 0.44 -0.4 4,977,468 - AO356_22255 0.44 +0.5 4,977,468 - AO356_22255 0.44 +0.1 4,977,468 - AO356_22255 0.44 +1.1 4,977,468 - AO356_22255 0.44 +0.8 4,977,468 - AO356_22255 0.44 -0.2 4,977,468 - AO356_22255 0.44 +0.3 4,977,513 - AO356_22255 0.47 +0.2 4,977,520 + AO356_22255 0.47 -0.6 4,977,520 + AO356_22255 0.47 -0.4 4,977,520 + AO356_22255 0.47 -0.2 4,977,521 - AO356_22255 0.47 -0.0 4,977,521 - AO356_22255 0.47 -0.3
Or see this region's nucleotide sequence