Strain Fitness in Pseudomonas fluorescens FW300-N2C3 around AO356_02500

Experiment: D-Cycloserine 0.1 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAO356_02490 and AO356_02495 are separated by 126 nucleotidesAO356_02495 and AO356_02500 are separated by 66 nucleotidesAO356_02500 and AO356_02505 are separated by 275 nucleotidesAO356_02505 and AO356_02510 are separated by 64 nucleotides AO356_02490: AO356_02490 - adenine phosphoribosyltransferase, at 540,956 to 541,504 _02490 AO356_02495: AO356_02495 - recombination protein RecR, at 541,631 to 542,233 _02495 AO356_02500: AO356_02500 - 2-alkenal reductase, at 542,300 to 543,334 _02500 AO356_02505: AO356_02505 - nucleoid-associated protein, at 543,610 to 543,948 _02505 AO356_02510: AO356_02510 - DNA polymerase III subunit gamma/tau, at 544,013 to 546,088 _02510 Position (kb) 542 543 544Strain fitness (log2 ratio) -3 -2 -1 0 1at 541.502 kb on + strandat 541.502 kb on + strandat 541.557 kb on + strandat 541.632 kb on + strandat 541.770 kb on + strand, within AO356_02495at 541.770 kb on + strand, within AO356_02495at 541.771 kb on - strand, within AO356_02495at 541.927 kb on - strand, within AO356_02495at 541.959 kb on + strand, within AO356_02495at 541.959 kb on + strand, within AO356_02495at 541.959 kb on + strand, within AO356_02495at 541.959 kb on + strand, within AO356_02495at 541.959 kb on + strand, within AO356_02495at 541.960 kb on - strand, within AO356_02495at 542.057 kb on + strand, within AO356_02495at 542.057 kb on + strand, within AO356_02495at 542.072 kb on + strand, within AO356_02495at 542.073 kb on - strand, within AO356_02495at 542.073 kb on - strand, within AO356_02495at 542.251 kb on + strandat 542.251 kb on + strandat 542.251 kb on + strandat 542.251 kb on + strandat 542.252 kb on - strandat 542.252 kb on - strandat 542.252 kb on - strandat 542.270 kb on - strandat 542.270 kb on - strandat 542.270 kb on - strandat 542.297 kb on + strandat 542.297 kb on + strandat 542.298 kb on - strandat 542.360 kb on - strandat 542.488 kb on + strand, within AO356_02500at 542.577 kb on + strand, within AO356_02500at 542.577 kb on + strand, within AO356_02500at 542.577 kb on + strand, within AO356_02500at 542.578 kb on - strand, within AO356_02500at 542.670 kb on + strand, within AO356_02500at 542.670 kb on + strand, within AO356_02500at 542.670 kb on + strand, within AO356_02500at 542.671 kb on - strand, within AO356_02500at 542.671 kb on - strand, within AO356_02500at 542.675 kb on - strand, within AO356_02500at 542.675 kb on - strand, within AO356_02500at 542.769 kb on + strand, within AO356_02500at 542.769 kb on + strand, within AO356_02500at 542.769 kb on + strand, within AO356_02500at 542.769 kb on + strand, within AO356_02500at 542.769 kb on + strand, within AO356_02500at 542.769 kb on + strand, within AO356_02500at 542.770 kb on - strand, within AO356_02500at 542.770 kb on - strand, within AO356_02500at 542.770 kb on - strand, within AO356_02500at 542.770 kb on - strand, within AO356_02500at 542.770 kb on - strand, within AO356_02500at 542.770 kb on - strand, within AO356_02500at 542.770 kb on - strand, within AO356_02500at 542.770 kb on - strand, within AO356_02500at 542.922 kb on + strand, within AO356_02500at 542.922 kb on + strand, within AO356_02500at 542.922 kb on + strand, within AO356_02500at 542.923 kb on - strand, within AO356_02500at 542.923 kb on - strand, within AO356_02500at 542.923 kb on - strand, within AO356_02500at 542.923 kb on - strand, within AO356_02500at 542.923 kb on - strand, within AO356_02500at 542.923 kb on - strand, within AO356_02500at 543.144 kb on + strand, within AO356_02500at 543.144 kb on + strand, within AO356_02500at 543.145 kb on - strand, within AO356_02500at 543.145 kb on - strand, within AO356_02500at 543.177 kb on + strand, within AO356_02500at 543.178 kb on - strand, within AO356_02500at 543.366 kb on + strandat 543.366 kb on + strandat 543.366 kb on + strandat 543.367 kb on - strandat 543.367 kb on - strandat 543.367 kb on - strandat 543.367 kb on - strandat 543.367 kb on - strandat 543.367 kb on - strandat 543.367 kb on - strandat 543.367 kb on - strandat 543.367 kb on - strandat 543.367 kb on - strandat 543.368 kb on + strandat 543.368 kb on + strandat 543.369 kb on - strandat 543.369 kb on - strandat 543.369 kb on - strandat 543.447 kb on + strandat 543.514 kb on + strandat 543.514 kb on + strandat 543.514 kb on + strandat 543.514 kb on + strandat 543.523 kb on + strandat 543.524 kb on - strandat 543.539 kb on + strandat 543.584 kb on + strandat 543.585 kb on - strandat 543.585 kb on - strandat 543.585 kb on - strandat 544.014 kb on + strandat 544.014 kb on + strandat 544.014 kb on + strandat 544.015 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml
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541,502 + -0.5
541,502 + -2.1
541,557 + -0.9
541,632 + +1.2
541,770 + AO356_02495 0.23 -1.8
541,770 + AO356_02495 0.23 -1.9
541,771 - AO356_02495 0.23 -0.6
541,927 - AO356_02495 0.49 -0.7
541,959 + AO356_02495 0.54 -0.6
541,959 + AO356_02495 0.54 -1.4
541,959 + AO356_02495 0.54 -0.4
541,959 + AO356_02495 0.54 -1.4
541,959 + AO356_02495 0.54 -3.4
541,960 - AO356_02495 0.55 +0.0
542,057 + AO356_02495 0.71 -0.9
542,057 + AO356_02495 0.71 -1.6
542,072 + AO356_02495 0.73 -2.1
542,073 - AO356_02495 0.73 -1.6
542,073 - AO356_02495 0.73 -0.6
542,251 + -1.4
542,251 + +0.4
542,251 + -2.3
542,251 + -1.2
542,252 - +0.9
542,252 - -0.1
542,252 - -0.6
542,270 - +0.5
542,270 - -0.1
542,270 - +0.6
542,297 + -0.5
542,297 + -0.0
542,298 - +0.4
542,360 - +0.2
542,488 + AO356_02500 0.18 +0.3
542,577 + AO356_02500 0.27 -0.0
542,577 + AO356_02500 0.27 -0.1
542,577 + AO356_02500 0.27 +0.7
542,578 - AO356_02500 0.27 +0.5
542,670 + AO356_02500 0.36 -1.5
542,670 + AO356_02500 0.36 +0.4
542,670 + AO356_02500 0.36 -0.7
542,671 - AO356_02500 0.36 +0.2
542,671 - AO356_02500 0.36 +0.1
542,675 - AO356_02500 0.36 +1.0
542,675 - AO356_02500 0.36 +0.4
542,769 + AO356_02500 0.45 +0.2
542,769 + AO356_02500 0.45 -0.1
542,769 + AO356_02500 0.45 -0.1
542,769 + AO356_02500 0.45 -0.1
542,769 + AO356_02500 0.45 -0.2
542,769 + AO356_02500 0.45 +0.4
542,770 - AO356_02500 0.45 -1.4
542,770 - AO356_02500 0.45 +0.3
542,770 - AO356_02500 0.45 -0.5
542,770 - AO356_02500 0.45 +0.4
542,770 - AO356_02500 0.45 +0.1
542,770 - AO356_02500 0.45 +0.3
542,770 - AO356_02500 0.45 +0.8
542,770 - AO356_02500 0.45 +0.0
542,922 + AO356_02500 0.60 -0.2
542,922 + AO356_02500 0.60 +1.0
542,922 + AO356_02500 0.60 -0.4
542,923 - AO356_02500 0.60 -0.3
542,923 - AO356_02500 0.60 -0.1
542,923 - AO356_02500 0.60 +0.0
542,923 - AO356_02500 0.60 -0.3
542,923 - AO356_02500 0.60 -0.7
542,923 - AO356_02500 0.60 +0.2
543,144 + AO356_02500 0.82 -0.1
543,144 + AO356_02500 0.82 -0.3
543,145 - AO356_02500 0.82 -0.5
543,145 - AO356_02500 0.82 -0.0
543,177 + AO356_02500 0.85 +0.5
543,178 - AO356_02500 0.85 +0.8
543,366 + +0.5
543,366 + -0.6
543,366 + -0.2
543,367 - -0.8
543,367 - -0.1
543,367 - +0.6
543,367 - -1.0
543,367 - -0.4
543,367 - -1.0
543,367 - -0.3
543,367 - +1.5
543,367 - +1.0
543,367 - +0.5
543,368 + +0.1
543,368 + -0.8
543,369 - +0.2
543,369 - +0.7
543,369 - -0.8
543,447 + -0.2
543,514 + -0.3
543,514 + -0.0
543,514 + +0.4
543,514 + -0.2
543,523 + -0.3
543,524 - +0.3
543,539 + -0.3
543,584 + -0.0
543,585 - +0.3
543,585 - -0.1
543,585 - +0.1
544,014 + +0.1
544,014 + +0.4
544,014 + -0.0
544,015 - +1.1

Or see this region's nucleotide sequence