Strain Fitness in Pseudomonas fluorescens FW300-N2C3 around AO356_02250

Experiment: D-Cycloserine 0.1 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAO356_02240 and AO356_02245 overlap by 4 nucleotidesAO356_02245 and AO356_02250 are separated by 42 nucleotidesAO356_02250 and AO356_02255 are separated by 211 nucleotides AO356_02240: AO356_02240 - pilus assembly protein PilZ, at 480,531 to 481,127 _02240 AO356_02245: AO356_02245 - glycerophosphodiester phosphodiesterase, at 481,124 to 481,846 _02245 AO356_02250: AO356_02250 - pyridine nucleotide-disulfide oxidoreductase, at 481,889 to 483,283 _02250 AO356_02255: AO356_02255 - thiamine biosynthesis protein ApbE, at 483,495 to 484,484 _02255 Position (kb) 481 482 483 484Strain fitness (log2 ratio) -3 -2 -1 0 1at 480.917 kb on - strand, within AO356_02240at 481.047 kb on + strand, within AO356_02240at 481.048 kb on - strand, within AO356_02240at 481.106 kb on + strandat 481.107 kb on - strandat 481.107 kb on - strandat 481.107 kb on - strandat 481.107 kb on - strandat 481.112 kb on + strandat 481.136 kb on + strandat 481.136 kb on + strandat 481.136 kb on + strandat 481.137 kb on - strandat 481.137 kb on - strandat 481.137 kb on - strandat 481.137 kb on - strandat 481.137 kb on - strandat 481.212 kb on + strand, within AO356_02245at 481.213 kb on - strand, within AO356_02245at 481.343 kb on + strand, within AO356_02245at 481.343 kb on + strand, within AO356_02245at 481.343 kb on + strand, within AO356_02245at 481.343 kb on + strand, within AO356_02245at 481.344 kb on - strand, within AO356_02245at 481.344 kb on - strand, within AO356_02245at 481.613 kb on + strand, within AO356_02245at 481.613 kb on + strand, within AO356_02245at 481.649 kb on + strand, within AO356_02245at 481.650 kb on - strand, within AO356_02245at 481.801 kb on + strandat 481.801 kb on + strandat 481.802 kb on - strandat 481.914 kb on + strandat 481.951 kb on - strandat 482.172 kb on + strand, within AO356_02250at 482.238 kb on + strand, within AO356_02250at 482.238 kb on + strand, within AO356_02250at 482.239 kb on - strand, within AO356_02250at 482.239 kb on - strand, within AO356_02250at 482.239 kb on - strand, within AO356_02250at 482.239 kb on - strand, within AO356_02250at 482.367 kb on + strand, within AO356_02250at 482.367 kb on + strand, within AO356_02250at 482.367 kb on + strand, within AO356_02250at 482.367 kb on + strand, within AO356_02250at 482.367 kb on + strand, within AO356_02250at 482.367 kb on + strand, within AO356_02250at 482.368 kb on - strand, within AO356_02250at 482.368 kb on - strand, within AO356_02250at 482.368 kb on - strand, within AO356_02250at 482.368 kb on - strand, within AO356_02250at 482.368 kb on - strand, within AO356_02250at 482.368 kb on - strand, within AO356_02250at 482.568 kb on + strand, within AO356_02250at 482.568 kb on + strand, within AO356_02250at 482.568 kb on + strand, within AO356_02250at 482.569 kb on - strand, within AO356_02250at 482.569 kb on - strand, within AO356_02250at 482.569 kb on - strand, within AO356_02250at 482.613 kb on + strand, within AO356_02250at 482.614 kb on - strand, within AO356_02250at 482.614 kb on - strand, within AO356_02250at 482.715 kb on + strand, within AO356_02250at 482.716 kb on - strand, within AO356_02250at 482.716 kb on - strand, within AO356_02250at 482.797 kb on - strand, within AO356_02250at 482.797 kb on - strand, within AO356_02250at 482.973 kb on + strand, within AO356_02250at 482.973 kb on + strand, within AO356_02250at 482.973 kb on + strand, within AO356_02250at 483.268 kb on - strandat 483.268 kb on - strandat 483.288 kb on - strandat 483.288 kb on - strandat 483.318 kb on - strandat 483.396 kb on + strandat 483.396 kb on + strandat 483.397 kb on - strandat 483.397 kb on - strandat 483.397 kb on - strandat 483.398 kb on + strandat 483.398 kb on + strandat 483.398 kb on + strandat 483.398 kb on + strandat 483.399 kb on - strandat 483.399 kb on - strandat 483.399 kb on - strandat 483.423 kb on - strandat 483.441 kb on - strandat 483.441 kb on - strandat 483.441 kb on - strandat 483.441 kb on - strandat 483.570 kb on + strandat 483.604 kb on + strand, within AO356_02255at 483.604 kb on + strand, within AO356_02255at 483.605 kb on - strand, within AO356_02255at 483.701 kb on + strand, within AO356_02255at 483.705 kb on + strand, within AO356_02255at 483.705 kb on + strand, within AO356_02255at 483.705 kb on + strand, within AO356_02255at 483.705 kb on + strand, within AO356_02255at 483.705 kb on + strand, within AO356_02255at 483.706 kb on - strand, within AO356_02255at 483.706 kb on - strand, within AO356_02255at 483.722 kb on + strand, within AO356_02255at 483.722 kb on + strand, within AO356_02255at 483.723 kb on - strand, within AO356_02255at 483.723 kb on - strand, within AO356_02255at 483.745 kb on + strand, within AO356_02255at 483.746 kb on - strand, within AO356_02255at 483.769 kb on + strand, within AO356_02255at 483.770 kb on - strand, within AO356_02255at 483.931 kb on + strand, within AO356_02255at 483.931 kb on + strand, within AO356_02255at 483.931 kb on + strand, within AO356_02255at 483.931 kb on + strand, within AO356_02255at 483.931 kb on + strand, within AO356_02255at 483.931 kb on + strand, within AO356_02255at 483.932 kb on - strand, within AO356_02255at 483.995 kb on + strand, within AO356_02255

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml
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480,917 - AO356_02240 0.65 +0.5
481,047 + AO356_02240 0.86 -1.1
481,048 - AO356_02240 0.87 +0.2
481,106 + +0.6
481,107 - +0.4
481,107 - +0.9
481,107 - -0.8
481,107 - +0.2
481,112 + +0.3
481,136 + +0.2
481,136 + +0.0
481,136 + -0.1
481,137 - -0.3
481,137 - -1.8
481,137 - -0.4
481,137 - +0.3
481,137 - +1.2
481,212 + AO356_02245 0.12 +0.1
481,213 - AO356_02245 0.12 -0.8
481,343 + AO356_02245 0.30 +1.3
481,343 + AO356_02245 0.30 +0.6
481,343 + AO356_02245 0.30 +1.7
481,343 + AO356_02245 0.30 +0.0
481,344 - AO356_02245 0.30 -0.8
481,344 - AO356_02245 0.30 -0.2
481,613 + AO356_02245 0.68 +0.6
481,613 + AO356_02245 0.68 -1.1
481,649 + AO356_02245 0.73 +1.0
481,650 - AO356_02245 0.73 -0.2
481,801 + -0.6
481,801 + -0.3
481,802 - +0.3
481,914 + -0.7
481,951 - -0.3
482,172 + AO356_02250 0.20 -0.4
482,238 + AO356_02250 0.25 -1.8
482,238 + AO356_02250 0.25 -2.1
482,239 - AO356_02250 0.25 -0.3
482,239 - AO356_02250 0.25 -1.5
482,239 - AO356_02250 0.25 +0.4
482,239 - AO356_02250 0.25 +0.5
482,367 + AO356_02250 0.34 -1.6
482,367 + AO356_02250 0.34 -0.6
482,367 + AO356_02250 0.34 +0.2
482,367 + AO356_02250 0.34 -1.7
482,367 + AO356_02250 0.34 -1.2
482,367 + AO356_02250 0.34 -3.0
482,368 - AO356_02250 0.34 -0.7
482,368 - AO356_02250 0.34 -0.6
482,368 - AO356_02250 0.34 -0.6
482,368 - AO356_02250 0.34 -0.3
482,368 - AO356_02250 0.34 -1.3
482,368 - AO356_02250 0.34 +0.5
482,568 + AO356_02250 0.49 -0.6
482,568 + AO356_02250 0.49 -0.4
482,568 + AO356_02250 0.49 -1.2
482,569 - AO356_02250 0.49 -0.6
482,569 - AO356_02250 0.49 -1.3
482,569 - AO356_02250 0.49 -1.1
482,613 + AO356_02250 0.52 -1.1
482,614 - AO356_02250 0.52 -1.1
482,614 - AO356_02250 0.52 +0.2
482,715 + AO356_02250 0.59 -1.1
482,716 - AO356_02250 0.59 -1.7
482,716 - AO356_02250 0.59 +1.0
482,797 - AO356_02250 0.65 -1.2
482,797 - AO356_02250 0.65 -2.0
482,973 + AO356_02250 0.78 -2.1
482,973 + AO356_02250 0.78 -1.0
482,973 + AO356_02250 0.78 -2.2
483,268 - -0.2
483,268 - -0.6
483,288 - -2.0
483,288 - -1.1
483,318 - +0.5
483,396 + +0.7
483,396 + -1.5
483,397 - +0.2
483,397 - +0.3
483,397 - -0.9
483,398 + +0.5
483,398 + +0.6
483,398 + +0.1
483,398 + -0.0
483,399 - +0.3
483,399 - -0.1
483,399 - +0.1
483,423 - -0.0
483,441 - +0.7
483,441 - -0.5
483,441 - -0.8
483,441 - +0.1
483,570 + +0.4
483,604 + AO356_02255 0.11 +0.0
483,604 + AO356_02255 0.11 -0.6
483,605 - AO356_02255 0.11 -0.7
483,701 + AO356_02255 0.21 -0.3
483,705 + AO356_02255 0.21 +0.3
483,705 + AO356_02255 0.21 -0.3
483,705 + AO356_02255 0.21 +0.4
483,705 + AO356_02255 0.21 +1.2
483,705 + AO356_02255 0.21 +0.1
483,706 - AO356_02255 0.21 +0.1
483,706 - AO356_02255 0.21 -0.6
483,722 + AO356_02255 0.23 +1.2
483,722 + AO356_02255 0.23 +0.0
483,723 - AO356_02255 0.23 +0.6
483,723 - AO356_02255 0.23 -0.2
483,745 + AO356_02255 0.25 +0.2
483,746 - AO356_02255 0.25 -0.0
483,769 + AO356_02255 0.28 +0.3
483,770 - AO356_02255 0.28 +0.3
483,931 + AO356_02255 0.44 -0.7
483,931 + AO356_02255 0.44 -0.6
483,931 + AO356_02255 0.44 +0.6
483,931 + AO356_02255 0.44 +0.3
483,931 + AO356_02255 0.44 -0.4
483,931 + AO356_02255 0.44 -0.1
483,932 - AO356_02255 0.44 -0.7
483,995 + AO356_02255 0.51 +0.8

Or see this region's nucleotide sequence