Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_02235 and AO356_02240 are separated by 94 nucleotides AO356_02240 and AO356_02245 overlap by 4 nucleotides AO356_02245 and AO356_02250 are separated by 42 nucleotides
AO356_02235: AO356_02235 - cell division protein FtsX, at 479,186 to 480,436
_02235
AO356_02240: AO356_02240 - pilus assembly protein PilZ, at 480,531 to 481,127
_02240
AO356_02245: AO356_02245 - glycerophosphodiester phosphodiesterase, at 481,124 to 481,846
_02245
AO356_02250: AO356_02250 - pyridine nucleotide-disulfide oxidoreductase, at 481,889 to 483,283
_02250
Position (kb)
481
482 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 480.497 kb on - strand at 480.497 kb on - strand at 480.497 kb on - strand at 480.497 kb on - strand at 480.542 kb on - strand at 480.542 kb on - strand at 480.542 kb on - strand at 480.542 kb on - strand at 480.565 kb on - strand at 480.750 kb on + strand, within AO356_02240 at 480.917 kb on - strand, within AO356_02240 at 481.047 kb on + strand, within AO356_02240 at 481.048 kb on - strand, within AO356_02240 at 481.106 kb on + strand at 481.107 kb on - strand at 481.107 kb on - strand at 481.107 kb on - strand at 481.107 kb on - strand at 481.112 kb on + strand at 481.136 kb on + strand at 481.136 kb on + strand at 481.136 kb on + strand at 481.137 kb on - strand at 481.137 kb on - strand at 481.137 kb on - strand at 481.137 kb on - strand at 481.137 kb on - strand at 481.212 kb on + strand, within AO356_02245 at 481.213 kb on - strand, within AO356_02245 at 481.343 kb on + strand, within AO356_02245 at 481.343 kb on + strand, within AO356_02245 at 481.343 kb on + strand, within AO356_02245 at 481.343 kb on + strand, within AO356_02245 at 481.344 kb on - strand, within AO356_02245 at 481.344 kb on - strand, within AO356_02245 at 481.613 kb on + strand, within AO356_02245 at 481.613 kb on + strand, within AO356_02245 at 481.649 kb on + strand, within AO356_02245 at 481.650 kb on - strand, within AO356_02245 at 481.801 kb on + strand at 481.801 kb on + strand at 481.802 kb on - strand at 481.914 kb on + strand at 481.951 kb on - strand at 482.172 kb on + strand, within AO356_02250 at 482.238 kb on + strand, within AO356_02250 at 482.238 kb on + strand, within AO356_02250 at 482.239 kb on - strand, within AO356_02250 at 482.239 kb on - strand, within AO356_02250 at 482.239 kb on - strand, within AO356_02250 at 482.239 kb on - strand, within AO356_02250 at 482.367 kb on + strand, within AO356_02250 at 482.367 kb on + strand, within AO356_02250 at 482.367 kb on + strand, within AO356_02250 at 482.367 kb on + strand, within AO356_02250 at 482.367 kb on + strand, within AO356_02250 at 482.367 kb on + strand, within AO356_02250 at 482.368 kb on - strand, within AO356_02250 at 482.368 kb on - strand, within AO356_02250 at 482.368 kb on - strand, within AO356_02250 at 482.368 kb on - strand, within AO356_02250 at 482.368 kb on - strand, within AO356_02250 at 482.368 kb on - strand, within AO356_02250 at 482.568 kb on + strand, within AO356_02250 at 482.568 kb on + strand, within AO356_02250 at 482.568 kb on + strand, within AO356_02250 at 482.569 kb on - strand, within AO356_02250 at 482.569 kb on - strand, within AO356_02250 at 482.569 kb on - strand, within AO356_02250 at 482.613 kb on + strand, within AO356_02250 at 482.614 kb on - strand, within AO356_02250 at 482.614 kb on - strand, within AO356_02250 at 482.715 kb on + strand, within AO356_02250 at 482.716 kb on - strand, within AO356_02250 at 482.716 kb on - strand, within AO356_02250 at 482.797 kb on - strand, within AO356_02250 at 482.797 kb on - strand, within AO356_02250
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 480,497 - +1.1 480,497 - -0.8 480,497 - -0.3 480,497 - -0.7 480,542 - +0.9 480,542 - -0.1 480,542 - +0.9 480,542 - -2.7 480,565 - +0.4 480,750 + AO356_02240 0.37 -0.9 480,917 - AO356_02240 0.65 +0.5 481,047 + AO356_02240 0.86 -1.1 481,048 - AO356_02240 0.87 +0.2 481,106 + +0.6 481,107 - +0.4 481,107 - +0.9 481,107 - -0.8 481,107 - +0.2 481,112 + +0.3 481,136 + +0.2 481,136 + +0.0 481,136 + -0.1 481,137 - -0.3 481,137 - -1.8 481,137 - -0.4 481,137 - +0.3 481,137 - +1.2 481,212 + AO356_02245 0.12 +0.1 481,213 - AO356_02245 0.12 -0.8 481,343 + AO356_02245 0.30 +1.3 481,343 + AO356_02245 0.30 +0.6 481,343 + AO356_02245 0.30 +1.7 481,343 + AO356_02245 0.30 +0.0 481,344 - AO356_02245 0.30 -0.8 481,344 - AO356_02245 0.30 -0.2 481,613 + AO356_02245 0.68 +0.6 481,613 + AO356_02245 0.68 -1.1 481,649 + AO356_02245 0.73 +1.0 481,650 - AO356_02245 0.73 -0.2 481,801 + -0.6 481,801 + -0.3 481,802 - +0.3 481,914 + -0.7 481,951 - -0.3 482,172 + AO356_02250 0.20 -0.4 482,238 + AO356_02250 0.25 -1.8 482,238 + AO356_02250 0.25 -2.1 482,239 - AO356_02250 0.25 -0.3 482,239 - AO356_02250 0.25 -1.5 482,239 - AO356_02250 0.25 +0.4 482,239 - AO356_02250 0.25 +0.5 482,367 + AO356_02250 0.34 -1.6 482,367 + AO356_02250 0.34 -0.6 482,367 + AO356_02250 0.34 +0.2 482,367 + AO356_02250 0.34 -1.7 482,367 + AO356_02250 0.34 -1.2 482,367 + AO356_02250 0.34 -3.0 482,368 - AO356_02250 0.34 -0.7 482,368 - AO356_02250 0.34 -0.6 482,368 - AO356_02250 0.34 -0.6 482,368 - AO356_02250 0.34 -0.3 482,368 - AO356_02250 0.34 -1.3 482,368 - AO356_02250 0.34 +0.5 482,568 + AO356_02250 0.49 -0.6 482,568 + AO356_02250 0.49 -0.4 482,568 + AO356_02250 0.49 -1.2 482,569 - AO356_02250 0.49 -0.6 482,569 - AO356_02250 0.49 -1.3 482,569 - AO356_02250 0.49 -1.1 482,613 + AO356_02250 0.52 -1.1 482,614 - AO356_02250 0.52 -1.1 482,614 - AO356_02250 0.52 +0.2 482,715 + AO356_02250 0.59 -1.1 482,716 - AO356_02250 0.59 -1.7 482,716 - AO356_02250 0.59 +1.0 482,797 - AO356_02250 0.65 -1.2 482,797 - AO356_02250 0.65 -2.0
Or see this region's nucleotide sequence