Experiment: L-Lysine 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3802 and gpsA are separated by 254 nucleotides gpsA and Shew_3804 are separated by 6 nucleotides Shew_3804 and Shew_3805 are separated by 207 nucleotides
Shew_3802: Shew_3802 - hypothetical protein (RefSeq), at 4,525,075 to 4,526,259
_3802
Shew_3803: gpsA - NAD(P)H-dependent glycerol-3-phosphate dehydrogenase (RefSeq), at 4,526,514 to 4,527,533
gpsA
Shew_3804: Shew_3804 - preprotein translocase subunit SecB (RefSeq), at 4,527,540 to 4,528,028
_3804
Shew_3805: Shew_3805 - rhodanese domain-containing protein (RefSeq), at 4,528,236 to 4,528,748
_3805
Position (kb)
4526
4527
4528 Strain fitness (log2 ratio)
-1
0
1 at 4525.570 kb on + strand, within Shew_3802 at 4525.679 kb on - strand, within Shew_3802 at 4525.807 kb on + strand, within Shew_3802 at 4525.807 kb on + strand, within Shew_3802 at 4525.814 kb on + strand, within Shew_3802 at 4526.209 kb on + strand at 4526.217 kb on - strand at 4526.372 kb on - strand at 4526.422 kb on + strand at 4526.435 kb on + strand at 4528.196 kb on - strand at 4528.307 kb on - strand, within Shew_3805 at 4528.307 kb on - strand, within Shew_3805 at 4528.361 kb on - strand, within Shew_3805 at 4528.371 kb on - strand, within Shew_3805 at 4528.489 kb on - strand, within Shew_3805
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lysine 200 mM remove 4,525,570 + Shew_3802 0.42 +0.2 4,525,679 - Shew_3802 0.51 -0.3 4,525,807 + Shew_3802 0.62 +0.6 4,525,807 + Shew_3802 0.62 +1.7 4,525,814 + Shew_3802 0.62 +0.6 4,526,209 + -0.2 4,526,217 - +0.0 4,526,372 - -0.1 4,526,422 + -0.3 4,526,435 + +0.6 4,528,196 - +0.3 4,528,307 - Shew_3805 0.14 -0.6 4,528,307 - Shew_3805 0.14 +0.4 4,528,361 - Shew_3805 0.24 -0.4 4,528,371 - Shew_3805 0.26 -1.1 4,528,489 - Shew_3805 0.49 -0.2
Or see this region's nucleotide sequence