Experiment: L-Lysine 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2890 and Shew_2891 are separated by 186 nucleotides Shew_2891 and Shew_2892 are separated by 145 nucleotides Shew_2892 and Shew_2893 are separated by 112 nucleotides Shew_2893 and Shew_2894 are separated by 157 nucleotides
Shew_2890: Shew_2890 - peptidyl-prolyl cis-trans isomerase, cyclophilin type (RefSeq), at 3,429,052 to 3,429,636
_2890
Shew_2891: Shew_2891 - hypothetical protein (RefSeq), at 3,429,823 to 3,430,155
_2891
Shew_2892: Shew_2892 - major facilitator transporter (RefSeq), at 3,430,301 to 3,431,719
_2892
Shew_2893: Shew_2893 - nucleotide-binding protein (RefSeq), at 3,431,832 to 3,432,317
_2893
Shew_2894: Shew_2894 - VanZ family protein (RefSeq), at 3,432,475 to 3,432,855
_2894
Position (kb)
3430
3431
3432 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3429.474 kb on - strand, within Shew_2890 at 3429.475 kb on - strand, within Shew_2890 at 3429.935 kb on + strand, within Shew_2891 at 3429.935 kb on + strand, within Shew_2891 at 3429.935 kb on + strand, within Shew_2891 at 3429.943 kb on - strand, within Shew_2891 at 3429.943 kb on - strand, within Shew_2891 at 3429.943 kb on - strand, within Shew_2891 at 3429.943 kb on - strand, within Shew_2891 at 3429.943 kb on - strand, within Shew_2891 at 3430.942 kb on - strand, within Shew_2892 at 3431.011 kb on + strand, within Shew_2892 at 3431.339 kb on + strand, within Shew_2892 at 3431.498 kb on - strand, within Shew_2892 at 3431.521 kb on - strand, within Shew_2892 at 3431.865 kb on + strand at 3431.963 kb on + strand, within Shew_2893 at 3432.026 kb on + strand, within Shew_2893 at 3432.051 kb on + strand, within Shew_2893 at 3432.111 kb on - strand, within Shew_2893 at 3432.462 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lysine 200 mM remove 3,429,474 - Shew_2890 0.72 -0.1 3,429,475 - Shew_2890 0.72 +0.8 3,429,935 + Shew_2891 0.34 +0.5 3,429,935 + Shew_2891 0.34 +0.6 3,429,935 + Shew_2891 0.34 +0.1 3,429,943 - Shew_2891 0.36 -0.1 3,429,943 - Shew_2891 0.36 +0.0 3,429,943 - Shew_2891 0.36 +0.4 3,429,943 - Shew_2891 0.36 +0.2 3,429,943 - Shew_2891 0.36 -1.1 3,430,942 - Shew_2892 0.45 -2.7 3,431,011 + Shew_2892 0.50 -2.3 3,431,339 + Shew_2892 0.73 -5.0 3,431,498 - Shew_2892 0.84 -2.8 3,431,521 - Shew_2892 0.86 -3.4 3,431,865 + +0.1 3,431,963 + Shew_2893 0.27 -0.0 3,432,026 + Shew_2893 0.40 -0.1 3,432,051 + Shew_2893 0.45 +0.1 3,432,111 - Shew_2893 0.57 -0.1 3,432,462 + -0.2
Or see this region's nucleotide sequence