Experiment: L-Lysine 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt uvrC and Shew_1826 are separated by 47 nucleotides Shew_1826 and Shew_R0061 are separated by 140 nucleotides Shew_R0061 and Shew_R0062 are separated by 12 nucleotides Shew_R0062 and Shew_R0063 are separated by 39 nucleotides Shew_R0063 and Shew_1827 are separated by 205 nucleotides
Shew_1825: uvrC - excinuclease ABC subunit C (RefSeq), at 2,099,943 to 2,101,772
uvrC
Shew_1826: Shew_1826 - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (RefSeq), at 2,101,820 to 2,102,377
_1826
Shew_R0061: Shew_R0061 - tRNA-Gly (RefSeq), at 2,102,518 to 2,102,593
_R0061
Shew_R0062: Shew_R0062 - tRNA-Cys (RefSeq), at 2,102,606 to 2,102,679
_R0062
Shew_R0063: Shew_R0063 - tRNA-Leu (RefSeq), at 2,102,719 to 2,102,805
_R0063
Shew_1827: Shew_1827 - methylation site containing protein (RefSeq), at 2,103,011 to 2,103,577
_1827
Position (kb)
2101
2102
2103 Strain fitness (log2 ratio)
-1
0
1
2 at 2100.978 kb on + strand, within uvrC at 2101.073 kb on + strand, within uvrC at 2101.496 kb on - strand, within uvrC at 2103.027 kb on + strand at 2103.027 kb on + strand at 2103.035 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lysine 200 mM remove 2,100,978 + uvrC Shew_1825 0.57 -0.7 2,101,073 + uvrC Shew_1825 0.62 -1.5 2,101,496 - uvrC Shew_1825 0.85 -0.6 2,103,027 + -0.2 2,103,027 + -0.6 2,103,035 - +1.8
Or see this region's nucleotide sequence