Experiment: L-Lysine 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt recA and Shew_1216 are separated by 79 nucleotides Shew_1216 and Shew_1217 are separated by 244 nucleotides Shew_1217 and Shew_1218 are separated by 306 nucleotides Shew_1218 and Shew_R0026 are separated by 213 nucleotides Shew_R0026 and Shew_R0027 are separated by 21 nucleotides Shew_R0027 and Shew_R0028 are separated by 37 nucleotides
Shew_1215: recA - recombinase A (RefSeq), at 1,410,941 to 1,412,002
recA
Shew_1216: Shew_1216 - regulatory protein RecX (RefSeq), at 1,412,082 to 1,412,534
_1216
Shew_1217: Shew_1217 - alanyl-tRNA synthetase (RefSeq), at 1,412,779 to 1,415,403
_1217
Shew_1218: Shew_1218 - carbon storage regulator, CsrA (RefSeq), at 1,415,710 to 1,415,907
_1218
Shew_R0026: Shew_R0026 - tRNA-Ser (RefSeq), at 1,416,121 to 1,416,212
_R0026
Shew_R0027: Shew_R0027 - tRNA-Arg (RefSeq), at 1,416,234 to 1,416,310
_R0027
Shew_R0028: Shew_R0028 - tRNA-Arg (RefSeq), at 1,416,348 to 1,416,424
_R0028
Position (kb)
1412
1413
1414
1415
1416 Strain fitness (log2 ratio)
-1
0
1 at 1412.153 kb on + strand, within Shew_1216 at 1412.291 kb on + strand, within Shew_1216 at 1412.294 kb on - strand, within Shew_1216 at 1412.304 kb on - strand, within Shew_1216 at 1412.455 kb on - strand, within Shew_1216 at 1415.469 kb on - strand at 1415.924 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lysine 200 mM remove 1,412,153 + Shew_1216 0.16 +0.2 1,412,291 + Shew_1216 0.46 -0.2 1,412,294 - Shew_1216 0.47 +0.1 1,412,304 - Shew_1216 0.49 -0.9 1,412,455 - Shew_1216 0.82 +0.1 1,415,469 - -0.1 1,415,924 + -0.5
Or see this region's nucleotide sequence