Experiment: L-Lysine 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0297 and Shew_0298 are separated by 125 nucleotides Shew_0298 and Shew_0299 are separated by 164 nucleotides Shew_0299 and Shew_0300 are separated by 130 nucleotides
Shew_0297: Shew_0297 - hypothetical protein (RefSeq), at 317,928 to 319,835
_0297
Shew_0298: Shew_0298 - Zn-dependent hydrolase of the beta-lactamase fold (RefSeq), at 319,961 to 321,049
_0298
Shew_0299: Shew_0299 - TetR family transcriptional regulator (RefSeq), at 321,214 to 321,822
_0299
Shew_0300: Shew_0300 - sodium:neurotransmitter symporter (RefSeq), at 321,953 to 323,311
_0300
Position (kb) 319
320
321
322 Strain fitness (log2 ratio) -1
0
1 at 319.211 kb on - strand, within Shew_0297 at 319.325 kb on + strand, within Shew_0297 at 319.541 kb on + strand, within Shew_0297 at 319.541 kb on + strand, within Shew_0297 at 319.977 kb on + strand at 320.184 kb on - strand, within Shew_0298 at 320.208 kb on - strand, within Shew_0298 at 320.230 kb on - strand, within Shew_0298 at 320.337 kb on - strand, within Shew_0298 at 320.463 kb on + strand, within Shew_0298 at 320.513 kb on + strand, within Shew_0298 at 320.773 kb on - strand, within Shew_0298 at 320.778 kb on + strand, within Shew_0298 at 320.791 kb on - strand, within Shew_0298 at 320.960 kb on - strand at 321.034 kb on + strand at 321.251 kb on - strand at 321.387 kb on + strand, within Shew_0299 at 321.528 kb on - strand, within Shew_0299 at 321.629 kb on + strand, within Shew_0299 at 321.725 kb on + strand, within Shew_0299 at 321.762 kb on + strand at 321.782 kb on + strand at 321.825 kb on - strand at 321.888 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lysine 200 mM remove 319,211 - Shew_0297 0.67 +0.5 319,325 + Shew_0297 0.73 -0.5 319,541 + Shew_0297 0.85 -0.4 319,541 + Shew_0297 0.85 -1.1 319,977 + +0.1 320,184 - Shew_0298 0.20 +0.5 320,208 - Shew_0298 0.23 -0.1 320,230 - Shew_0298 0.25 +0.1 320,337 - Shew_0298 0.35 -0.6 320,463 + Shew_0298 0.46 +0.3 320,513 + Shew_0298 0.51 +0.0 320,773 - Shew_0298 0.75 +0.3 320,778 + Shew_0298 0.75 -0.1 320,791 - Shew_0298 0.76 -0.2 320,960 - +0.1 321,034 + +0.3 321,251 - +0.1 321,387 + Shew_0299 0.28 -0.1 321,528 - Shew_0299 0.52 +0.2 321,629 + Shew_0299 0.68 -0.3 321,725 + Shew_0299 0.84 -0.5 321,762 + +0.3 321,782 + +0.7 321,825 - +1.0 321,888 - -0.1
Or see this region's nucleotide sequence