Experiment: BHIS with Cephalothin 0.625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4476 and BT4477 are separated by 34 nucleotides BT4477 and BT4478 are separated by 192 nucleotides
BT4476: BT4476 - amino acid carrier protein (NCBI ptt file), at 5,889,212 to 5,890,600
BT4476
BT4477: BT4477 - putative ATP-dependent DNA helicase (NCBI ptt file), at 5,890,635 to 5,891,282
BT4477
BT4478: BT4478 - hypothetical protein (NCBI ptt file), at 5,891,475 to 5,891,756
BT4478
Position (kb)
5890
5891
5892 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 5889.667 kb on + strand, within BT4476 at 5889.668 kb on - strand, within BT4476 at 5889.693 kb on - strand, within BT4476 at 5889.722 kb on + strand, within BT4476 at 5889.723 kb on - strand, within BT4476 at 5889.807 kb on - strand, within BT4476 at 5889.930 kb on - strand, within BT4476 at 5889.955 kb on - strand, within BT4476 at 5889.955 kb on - strand, within BT4476 at 5889.989 kb on + strand, within BT4476 at 5889.990 kb on - strand, within BT4476 at 5889.990 kb on - strand, within BT4476 at 5889.990 kb on - strand, within BT4476 at 5889.990 kb on - strand, within BT4476 at 5890.141 kb on + strand, within BT4476 at 5890.201 kb on + strand, within BT4476 at 5890.203 kb on + strand, within BT4476 at 5890.204 kb on - strand, within BT4476 at 5890.204 kb on - strand, within BT4476 at 5890.246 kb on + strand, within BT4476 at 5890.250 kb on + strand, within BT4476 at 5890.265 kb on - strand, within BT4476 at 5890.466 kb on + strand at 5890.467 kb on - strand at 5890.470 kb on - strand at 5890.489 kb on + strand at 5890.489 kb on + strand at 5890.561 kb on - strand at 5890.561 kb on - strand at 5890.572 kb on - strand at 5890.572 kb on - strand at 5890.572 kb on - strand at 5890.600 kb on + strand at 5890.600 kb on + strand at 5890.631 kb on + strand at 5890.633 kb on + strand at 5890.634 kb on - strand at 5890.634 kb on - strand at 5890.823 kb on + strand, within BT4477 at 5890.893 kb on + strand, within BT4477 at 5890.893 kb on + strand, within BT4477 at 5890.941 kb on + strand, within BT4477 at 5891.014 kb on + strand, within BT4477 at 5891.014 kb on + strand, within BT4477 at 5891.098 kb on - strand, within BT4477 at 5891.250 kb on - strand at 5891.676 kb on - strand, within BT4478 at 5891.679 kb on + strand, within BT4478 at 5891.682 kb on - strand, within BT4478 at 5891.739 kb on - strand at 5891.739 kb on - strand at 5891.847 kb on - strand at 5891.992 kb on + strand at 5892.004 kb on - strand at 5892.118 kb on - strand at 5892.259 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Cephalothin 0.625 mg/ml remove 5,889,667 + BT4476 0.33 +1.3 5,889,668 - BT4476 0.33 +1.5 5,889,693 - BT4476 0.35 +0.7 5,889,722 + BT4476 0.37 -0.5 5,889,723 - BT4476 0.37 +1.3 5,889,807 - BT4476 0.43 -2.5 5,889,930 - BT4476 0.52 +0.6 5,889,955 - BT4476 0.53 -0.9 5,889,955 - BT4476 0.53 -1.1 5,889,989 + BT4476 0.56 -1.4 5,889,990 - BT4476 0.56 -1.5 5,889,990 - BT4476 0.56 -0.6 5,889,990 - BT4476 0.56 -1.5 5,889,990 - BT4476 0.56 -0.3 5,890,141 + BT4476 0.67 +0.1 5,890,201 + BT4476 0.71 -0.4 5,890,203 + BT4476 0.71 -1.7 5,890,204 - BT4476 0.71 -1.6 5,890,204 - BT4476 0.71 +1.4 5,890,246 + BT4476 0.74 -0.4 5,890,250 + BT4476 0.75 -0.7 5,890,265 - BT4476 0.76 +0.6 5,890,466 + +0.1 5,890,467 - -0.9 5,890,470 - -0.6 5,890,489 + -0.1 5,890,489 + -1.7 5,890,561 - -0.4 5,890,561 - +2.7 5,890,572 - +0.9 5,890,572 - +0.1 5,890,572 - -2.0 5,890,600 + -1.1 5,890,600 + -2.8 5,890,631 + +0.1 5,890,633 + -3.7 5,890,634 - +1.6 5,890,634 - +1.3 5,890,823 + BT4477 0.29 +1.1 5,890,893 + BT4477 0.40 -1.7 5,890,893 + BT4477 0.40 -4.1 5,890,941 + BT4477 0.47 -1.7 5,891,014 + BT4477 0.58 -2.2 5,891,014 + BT4477 0.58 +1.1 5,891,098 - BT4477 0.71 +0.5 5,891,250 - -0.5 5,891,676 - BT4478 0.71 -0.5 5,891,679 + BT4478 0.72 -1.8 5,891,682 - BT4478 0.73 +0.5 5,891,739 - -0.5 5,891,739 - -0.5 5,891,847 - +0.8 5,891,992 + +1.1 5,892,004 - -2.6 5,892,118 - -0.1 5,892,259 + +0.8
Or see this region's nucleotide sequence