Experiment: BHIS with Cephalothin 0.625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4194 and BT4195 are separated by 27 nucleotides BT4195 and BT4196 are separated by 78 nucleotides BT4196 and BT4197 are separated by 89 nucleotides BT4197 and BT4198 are separated by 197 nucleotides BT4198 and BT4199 are separated by 34 nucleotides
BT4194: BT4194 - putative integral membrane protein (NCBI ptt file), at 5,530,454 to 5,531,401
BT4194
BT4195: BT4195 - conserved hypothetical protein (NCBI ptt file), at 5,531,429 to 5,531,641
BT4195
BT4196: BT4196 - hypothetical protein (NCBI ptt file), at 5,531,720 to 5,532,022
BT4196
BT4197: BT4197 - hypothetical protein (NCBI ptt file), at 5,532,112 to 5,532,417
BT4197
BT4198: BT4198 - conserved hypothetical protein (NCBI ptt file), at 5,532,615 to 5,532,980
BT4198
BT4199: BT4199 - conserved hypothetical protein (NCBI ptt file), at 5,533,015 to 5,534,631
BT4199
Position (kb)
5532
5533 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 5531.128 kb on - strand, within BT4194 at 5531.141 kb on - strand, within BT4194 at 5531.143 kb on + strand, within BT4194 at 5531.143 kb on + strand, within BT4194 at 5531.144 kb on - strand, within BT4194 at 5531.253 kb on + strand, within BT4194 at 5531.254 kb on - strand, within BT4194 at 5531.420 kb on - strand at 5531.472 kb on + strand, within BT4195 at 5531.551 kb on + strand, within BT4195 at 5531.640 kb on - strand at 5531.669 kb on + strand at 5531.748 kb on - strand at 5531.772 kb on + strand, within BT4196 at 5532.008 kb on - strand at 5532.021 kb on - strand at 5532.024 kb on - strand at 5532.062 kb on - strand at 5532.063 kb on + strand at 5532.064 kb on - strand at 5532.064 kb on - strand at 5532.074 kb on + strand at 5532.075 kb on - strand at 5532.075 kb on - strand at 5532.098 kb on + strand at 5532.099 kb on - strand at 5532.101 kb on - strand at 5532.101 kb on - strand at 5532.185 kb on + strand, within BT4197 at 5532.198 kb on + strand, within BT4197 at 5532.208 kb on - strand, within BT4197 at 5532.215 kb on + strand, within BT4197 at 5532.234 kb on - strand, within BT4197 at 5532.237 kb on + strand, within BT4197 at 5532.240 kb on + strand, within BT4197 at 5532.282 kb on - strand, within BT4197 at 5532.344 kb on + strand, within BT4197 at 5532.345 kb on - strand, within BT4197 at 5532.367 kb on - strand, within BT4197 at 5532.377 kb on + strand, within BT4197 at 5532.401 kb on - strand at 5532.489 kb on - strand at 5532.489 kb on - strand at 5532.498 kb on + strand at 5532.599 kb on - strand at 5532.610 kb on + strand at 5532.614 kb on + strand at 5532.631 kb on + strand at 5532.632 kb on - strand at 5532.737 kb on + strand, within BT4198 at 5532.782 kb on + strand, within BT4198 at 5532.783 kb on - strand, within BT4198 at 5532.799 kb on - strand, within BT4198 at 5532.808 kb on - strand, within BT4198 at 5532.867 kb on + strand, within BT4198 at 5532.876 kb on - strand, within BT4198 at 5532.949 kb on + strand at 5533.012 kb on - strand at 5533.034 kb on + strand at 5533.034 kb on + strand at 5533.035 kb on - strand at 5533.036 kb on + strand at 5533.037 kb on - strand at 5533.038 kb on + strand at 5533.038 kb on + strand at 5533.039 kb on - strand at 5533.294 kb on - strand, within BT4199 at 5533.294 kb on - strand, within BT4199 at 5533.294 kb on - strand, within BT4199 at 5533.298 kb on + strand, within BT4199 at 5533.299 kb on - strand, within BT4199 at 5533.299 kb on - strand, within BT4199 at 5533.299 kb on - strand, within BT4199 at 5533.302 kb on + strand, within BT4199 at 5533.400 kb on - strand, within BT4199 at 5533.413 kb on - strand, within BT4199
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Cephalothin 0.625 mg/ml remove 5,531,128 - BT4194 0.71 +0.5 5,531,141 - BT4194 0.72 +0.1 5,531,143 + BT4194 0.73 +0.6 5,531,143 + BT4194 0.73 -1.2 5,531,144 - BT4194 0.73 -2.3 5,531,253 + BT4194 0.84 -0.5 5,531,254 - BT4194 0.84 +0.6 5,531,420 - -0.2 5,531,472 + BT4195 0.20 -1.9 5,531,551 + BT4195 0.57 -3.0 5,531,640 - +0.1 5,531,669 + -1.7 5,531,748 - +0.6 5,531,772 + BT4196 0.17 -1.3 5,532,008 - -1.7 5,532,021 - -2.8 5,532,024 - -0.7 5,532,062 - +1.1 5,532,063 + +2.7 5,532,064 - -1.6 5,532,064 - -0.3 5,532,074 + -0.2 5,532,075 - -2.6 5,532,075 - -1.5 5,532,098 + -1.2 5,532,099 - +0.0 5,532,101 - -0.8 5,532,101 - +1.1 5,532,185 + BT4197 0.24 -1.4 5,532,198 + BT4197 0.28 -0.7 5,532,208 - BT4197 0.31 -0.9 5,532,215 + BT4197 0.34 -2.9 5,532,234 - BT4197 0.40 -1.5 5,532,237 + BT4197 0.41 +0.1 5,532,240 + BT4197 0.42 -0.6 5,532,282 - BT4197 0.56 -2.8 5,532,344 + BT4197 0.76 -1.7 5,532,345 - BT4197 0.76 -1.0 5,532,367 - BT4197 0.83 +0.6 5,532,377 + BT4197 0.87 -1.5 5,532,401 - -0.5 5,532,489 - -0.2 5,532,489 - -1.2 5,532,498 + -1.0 5,532,599 - +0.1 5,532,610 + +0.1 5,532,614 + +1.3 5,532,631 + +0.3 5,532,632 - +1.1 5,532,737 + BT4198 0.33 -2.0 5,532,782 + BT4198 0.46 -0.0 5,532,783 - BT4198 0.46 -0.6 5,532,799 - BT4198 0.50 -1.7 5,532,808 - BT4198 0.53 -0.0 5,532,867 + BT4198 0.69 -1.2 5,532,876 - BT4198 0.71 -0.9 5,532,949 + +0.7 5,533,012 - +0.5 5,533,034 + +1.1 5,533,034 + -1.2 5,533,035 - -0.9 5,533,036 + +0.6 5,533,037 - -0.2 5,533,038 + +0.8 5,533,038 + -0.1 5,533,039 - -0.7 5,533,294 - BT4199 0.17 -2.2 5,533,294 - BT4199 0.17 -0.6 5,533,294 - BT4199 0.17 -0.9 5,533,298 + BT4199 0.18 -3.3 5,533,299 - BT4199 0.18 +0.1 5,533,299 - BT4199 0.18 -0.9 5,533,299 - BT4199 0.18 -1.3 5,533,302 + BT4199 0.18 -1.2 5,533,400 - BT4199 0.24 -3.2 5,533,413 - BT4199 0.25 -4.7
Or see this region's nucleotide sequence