Experiment: BHIS with Cephalothin 0.625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4141 and BT4142 are separated by 819 nucleotides BT4142 and BT4143 overlap by 4 nucleotides BT4143 and BT4144 are separated by 138 nucleotides
BT4141: BT4141 - hypothetical protein (NCBI ptt file), at 5,436,642 to 5,436,974
BT4141
BT4142: BT4142 - conserved hypothetical protein (NCBI ptt file), at 5,437,794 to 5,438,921
BT4142
BT4143: BT4143 - putative iron-sulfur cluster binding protein (NCBI ptt file), at 5,438,918 to 5,439,598
BT4143
BT4144: BT4144 - transposase (NCBI ptt file), at 5,439,737 to 5,440,846
BT4144
Position (kb) 5437
5438
5439 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 5436.801 kb on - strand, within BT4141 at 5436.845 kb on - strand, within BT4141 at 5436.855 kb on + strand, within BT4141 at 5436.856 kb on - strand, within BT4141 at 5436.876 kb on - strand, within BT4141 at 5436.902 kb on - strand, within BT4141 at 5436.902 kb on - strand, within BT4141 at 5436.902 kb on - strand, within BT4141 at 5436.902 kb on - strand, within BT4141 at 5436.904 kb on - strand, within BT4141 at 5436.955 kb on + strand at 5436.955 kb on + strand at 5437.077 kb on - strand at 5437.143 kb on + strand at 5437.183 kb on + strand at 5437.203 kb on - strand at 5437.249 kb on + strand at 5437.252 kb on + strand at 5437.269 kb on - strand at 5437.269 kb on - strand at 5437.282 kb on - strand at 5437.296 kb on + strand at 5437.546 kb on - strand at 5437.546 kb on - strand at 5437.546 kb on - strand at 5437.546 kb on - strand at 5437.549 kb on + strand at 5437.549 kb on + strand at 5437.550 kb on - strand at 5437.591 kb on + strand at 5437.696 kb on + strand at 5437.697 kb on - strand at 5437.697 kb on - strand at 5437.706 kb on - strand at 5437.706 kb on - strand at 5437.759 kb on + strand at 5437.760 kb on - strand at 5437.766 kb on + strand at 5437.819 kb on - strand at 5437.888 kb on - strand at 5437.923 kb on + strand, within BT4142 at 5437.950 kb on + strand, within BT4142 at 5437.980 kb on + strand, within BT4142 at 5437.997 kb on - strand, within BT4142 at 5438.028 kb on - strand, within BT4142 at 5438.092 kb on - strand, within BT4142 at 5438.188 kb on - strand, within BT4142 at 5438.238 kb on + strand, within BT4142 at 5438.281 kb on + strand, within BT4142 at 5438.294 kb on - strand, within BT4142 at 5438.340 kb on - strand, within BT4142 at 5438.340 kb on - strand, within BT4142 at 5438.340 kb on - strand, within BT4142 at 5438.340 kb on - strand, within BT4142 at 5438.340 kb on - strand, within BT4142 at 5438.379 kb on - strand, within BT4142 at 5438.451 kb on - strand, within BT4142 at 5438.482 kb on - strand, within BT4142 at 5438.482 kb on - strand, within BT4142 at 5438.546 kb on - strand, within BT4142 at 5438.562 kb on + strand, within BT4142 at 5438.586 kb on - strand, within BT4142 at 5438.586 kb on - strand, within BT4142 at 5438.589 kb on + strand, within BT4142 at 5438.594 kb on - strand, within BT4142 at 5438.610 kb on + strand, within BT4142 at 5438.611 kb on - strand, within BT4142 at 5438.611 kb on - strand, within BT4142 at 5438.646 kb on - strand, within BT4142 at 5438.656 kb on + strand, within BT4142 at 5438.656 kb on + strand, within BT4142 at 5438.657 kb on - strand, within BT4142 at 5438.663 kb on - strand, within BT4142 at 5438.700 kb on + strand, within BT4142 at 5438.700 kb on + strand, within BT4142 at 5438.721 kb on + strand, within BT4142 at 5438.722 kb on - strand, within BT4142 at 5438.726 kb on + strand, within BT4142 at 5438.728 kb on + strand, within BT4142 at 5438.728 kb on + strand, within BT4142 at 5438.746 kb on + strand, within BT4142 at 5438.747 kb on - strand, within BT4142 at 5438.748 kb on + strand, within BT4142 at 5438.749 kb on - strand, within BT4142 at 5438.749 kb on - strand, within BT4142 at 5438.762 kb on + strand, within BT4142 at 5438.767 kb on + strand, within BT4142 at 5438.769 kb on + strand, within BT4142 at 5438.777 kb on + strand, within BT4142 at 5438.782 kb on - strand, within BT4142 at 5438.841 kb on + strand at 5438.849 kb on + strand at 5438.849 kb on + strand at 5438.917 kb on + strand at 5438.923 kb on + strand at 5438.974 kb on + strand at 5438.976 kb on + strand at 5438.980 kb on + strand at 5438.981 kb on - strand at 5438.998 kb on + strand, within BT4143 at 5439.007 kb on + strand, within BT4143 at 5439.025 kb on + strand, within BT4143 at 5439.026 kb on - strand, within BT4143 at 5439.028 kb on - strand, within BT4143 at 5439.030 kb on + strand, within BT4143 at 5439.031 kb on - strand, within BT4143 at 5439.089 kb on - strand, within BT4143 at 5439.110 kb on + strand, within BT4143 at 5439.114 kb on + strand, within BT4143 at 5439.128 kb on - strand, within BT4143 at 5439.141 kb on + strand, within BT4143 at 5439.142 kb on - strand, within BT4143 at 5439.153 kb on + strand, within BT4143 at 5439.181 kb on + strand, within BT4143 at 5439.181 kb on + strand, within BT4143 at 5439.181 kb on + strand, within BT4143 at 5439.181 kb on + strand, within BT4143 at 5439.280 kb on + strand, within BT4143 at 5439.389 kb on + strand, within BT4143 at 5439.422 kb on - strand, within BT4143 at 5439.425 kb on - strand, within BT4143 at 5439.439 kb on - strand, within BT4143 at 5439.477 kb on + strand, within BT4143 at 5439.497 kb on - strand, within BT4143 at 5439.507 kb on + strand, within BT4143 at 5439.507 kb on + strand, within BT4143 at 5439.507 kb on + strand, within BT4143 at 5439.508 kb on - strand, within BT4143 at 5439.508 kb on - strand, within BT4143 at 5439.508 kb on - strand, within BT4143 at 5439.508 kb on - strand, within BT4143 at 5439.509 kb on + strand, within BT4143 at 5439.510 kb on - strand, within BT4143 at 5439.520 kb on + strand, within BT4143 at 5439.563 kb on + strand at 5439.563 kb on + strand at 5439.563 kb on + strand at 5439.564 kb on - strand at 5439.589 kb on - strand at 5439.596 kb on + strand at 5439.606 kb on - strand at 5439.636 kb on - strand at 5439.643 kb on - strand at 5439.650 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Cephalothin 0.625 mg/ml remove 5,436,801 - BT4141 0.48 -0.9 5,436,845 - BT4141 0.61 -1.8 5,436,855 + BT4141 0.64 -1.6 5,436,856 - BT4141 0.64 -1.3 5,436,876 - BT4141 0.70 +0.6 5,436,902 - BT4141 0.78 -0.3 5,436,902 - BT4141 0.78 +0.2 5,436,902 - BT4141 0.78 -2.0 5,436,902 - BT4141 0.78 -0.7 5,436,904 - BT4141 0.79 -0.1 5,436,955 + +0.3 5,436,955 + +0.2 5,437,077 - +0.0 5,437,143 + +0.4 5,437,183 + +0.8 5,437,203 - +0.2 5,437,249 + -1.7 5,437,252 + -0.3 5,437,269 - +2.0 5,437,269 - -0.5 5,437,282 - -0.5 5,437,296 + +0.2 5,437,546 - -0.6 5,437,546 - -0.8 5,437,546 - -2.5 5,437,546 - -0.8 5,437,549 + -0.6 5,437,549 + -1.4 5,437,550 - -1.6 5,437,591 + -1.1 5,437,696 + -0.8 5,437,697 - +0.9 5,437,697 - -0.1 5,437,706 - +0.2 5,437,706 - -2.5 5,437,759 + -0.4 5,437,760 - +0.2 5,437,766 + -1.8 5,437,819 - -1.1 5,437,888 - -1.5 5,437,923 + BT4142 0.11 -1.2 5,437,950 + BT4142 0.14 -1.2 5,437,980 + BT4142 0.16 -2.0 5,437,997 - BT4142 0.18 -0.0 5,438,028 - BT4142 0.21 -0.6 5,438,092 - BT4142 0.26 +0.2 5,438,188 - BT4142 0.35 -1.0 5,438,238 + BT4142 0.39 -2.7 5,438,281 + BT4142 0.43 +0.0 5,438,294 - BT4142 0.44 -1.1 5,438,340 - BT4142 0.48 -2.4 5,438,340 - BT4142 0.48 -3.0 5,438,340 - BT4142 0.48 -1.6 5,438,340 - BT4142 0.48 +0.0 5,438,340 - BT4142 0.48 +0.2 5,438,379 - BT4142 0.52 -1.1 5,438,451 - BT4142 0.58 +1.2 5,438,482 - BT4142 0.61 -3.2 5,438,482 - BT4142 0.61 +0.3 5,438,546 - BT4142 0.67 -1.5 5,438,562 + BT4142 0.68 +0.7 5,438,586 - BT4142 0.70 +0.7 5,438,586 - BT4142 0.70 -0.8 5,438,589 + BT4142 0.70 -1.6 5,438,594 - BT4142 0.71 -1.0 5,438,610 + BT4142 0.72 -1.4 5,438,611 - BT4142 0.72 -3.7 5,438,611 - BT4142 0.72 +0.8 5,438,646 - BT4142 0.76 -1.1 5,438,656 + BT4142 0.76 -1.4 5,438,656 + BT4142 0.76 +0.6 5,438,657 - BT4142 0.77 -3.6 5,438,663 - BT4142 0.77 -1.2 5,438,700 + BT4142 0.80 -1.4 5,438,700 + BT4142 0.80 -2.6 5,438,721 + BT4142 0.82 -2.2 5,438,722 - BT4142 0.82 +0.2 5,438,726 + BT4142 0.83 -1.0 5,438,728 + BT4142 0.83 -1.2 5,438,728 + BT4142 0.83 +0.6 5,438,746 + BT4142 0.84 -1.4 5,438,747 - BT4142 0.84 -1.6 5,438,748 + BT4142 0.85 +0.6 5,438,749 - BT4142 0.85 -3.0 5,438,749 - BT4142 0.85 -1.1 5,438,762 + BT4142 0.86 -1.8 5,438,767 + BT4142 0.86 +0.6 5,438,769 + BT4142 0.86 +1.0 5,438,777 + BT4142 0.87 -1.0 5,438,782 - BT4142 0.88 -1.3 5,438,841 + -0.3 5,438,849 + -1.2 5,438,849 + -2.8 5,438,917 + -0.4 5,438,923 + -1.4 5,438,974 + +0.9 5,438,976 + -1.6 5,438,980 + -0.4 5,438,981 - -0.7 5,438,998 + BT4143 0.12 -0.1 5,439,007 + BT4143 0.13 -3.5 5,439,025 + BT4143 0.16 -0.6 5,439,026 - BT4143 0.16 +0.2 5,439,028 - BT4143 0.16 -1.0 5,439,030 + BT4143 0.16 -0.7 5,439,031 - BT4143 0.17 +0.2 5,439,089 - BT4143 0.25 -0.6 5,439,110 + BT4143 0.28 -4.4 5,439,114 + BT4143 0.29 +1.2 5,439,128 - BT4143 0.31 -1.0 5,439,141 + BT4143 0.33 -1.8 5,439,142 - BT4143 0.33 +0.6 5,439,153 + BT4143 0.35 -2.9 5,439,181 + BT4143 0.39 +0.9 5,439,181 + BT4143 0.39 -0.3 5,439,181 + BT4143 0.39 -0.5 5,439,181 + BT4143 0.39 -1.8 5,439,280 + BT4143 0.53 +0.4 5,439,389 + BT4143 0.69 +0.8 5,439,422 - BT4143 0.74 +1.0 5,439,425 - BT4143 0.74 -0.0 5,439,439 - BT4143 0.77 +0.2 5,439,477 + BT4143 0.82 +1.2 5,439,497 - BT4143 0.85 +0.7 5,439,507 + BT4143 0.86 -1.2 5,439,507 + BT4143 0.86 -2.0 5,439,507 + BT4143 0.86 -0.4 5,439,508 - BT4143 0.87 +0.2 5,439,508 - BT4143 0.87 -2.3 5,439,508 - BT4143 0.87 -1.2 5,439,508 - BT4143 0.87 -0.4 5,439,509 + BT4143 0.87 -3.2 5,439,510 - BT4143 0.87 -1.2 5,439,520 + BT4143 0.88 -1.6 5,439,563 + +0.8 5,439,563 + -0.7 5,439,563 + +2.0 5,439,564 - -0.3 5,439,589 - -1.3 5,439,596 + +1.2 5,439,606 - -0.4 5,439,636 - -2.0 5,439,643 - +1.2 5,439,650 - -2.3
Or see this region's nucleotide sequence