Experiment: BHIS with Cephalothin 0.625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3284 and BT3285 are separated by 612 nucleotides BT3285 and BT3286 are separated by 106 nucleotides BT3286 and BT3287 are separated by 35 nucleotides
BT3284: BT3284 - conserved hypothetical protein (NCBI ptt file), at 4,200,610 to 4,201,842
BT3284
BT3285: BT3285 - Holliday junction DNA helicase ruvB (NCBI ptt file), at 4,202,455 to 4,203,486
BT3285
BT3286: BT3286 - conserved hypothetical protein (NCBI ptt file), at 4,203,593 to 4,204,360
BT3286
BT3287: BT3287 - polysaccharide biosynthesis protein (NCBI ptt file), at 4,204,396 to 4,205,844
BT3287
Position (kb)
4202
4203
4204 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4201.700 kb on - strand, within BT3284 at 4201.750 kb on - strand at 4201.859 kb on - strand at 4201.861 kb on - strand at 4201.861 kb on - strand at 4201.861 kb on - strand at 4201.861 kb on - strand at 4201.920 kb on + strand at 4201.967 kb on + strand at 4202.008 kb on + strand at 4202.032 kb on - strand at 4202.041 kb on - strand at 4202.041 kb on - strand at 4202.079 kb on + strand at 4202.085 kb on + strand at 4202.086 kb on - strand at 4202.109 kb on + strand at 4202.110 kb on - strand at 4202.110 kb on - strand at 4202.111 kb on + strand at 4202.345 kb on - strand at 4203.487 kb on + strand at 4203.487 kb on + strand at 4203.607 kb on + strand at 4203.612 kb on + strand at 4203.612 kb on + strand at 4203.618 kb on + strand at 4203.642 kb on + strand at 4203.672 kb on + strand, within BT3286 at 4203.867 kb on + strand, within BT3286 at 4203.997 kb on + strand, within BT3286 at 4204.001 kb on + strand, within BT3286 at 4204.037 kb on + strand, within BT3286 at 4204.039 kb on + strand, within BT3286 at 4204.268 kb on + strand, within BT3286
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Cephalothin 0.625 mg/ml remove 4,201,700 - BT3284 0.88 -2.8 4,201,750 - -1.4 4,201,859 - -0.7 4,201,861 - -0.3 4,201,861 - +0.4 4,201,861 - -1.8 4,201,861 - -0.1 4,201,920 + -1.2 4,201,967 + -0.7 4,202,008 + +0.9 4,202,032 - -2.7 4,202,041 - -0.2 4,202,041 - +0.5 4,202,079 + -0.1 4,202,085 + +1.2 4,202,086 - -0.6 4,202,109 + +0.7 4,202,110 - -1.9 4,202,110 - -3.3 4,202,111 + -0.6 4,202,345 - -0.7 4,203,487 + -2.1 4,203,487 + +2.0 4,203,607 + +0.1 4,203,612 + -2.5 4,203,612 + -1.8 4,203,618 + +1.4 4,203,642 + +0.4 4,203,672 + BT3286 0.10 -1.2 4,203,867 + BT3286 0.36 -0.0 4,203,997 + BT3286 0.53 -2.6 4,204,001 + BT3286 0.53 +0.8 4,204,037 + BT3286 0.58 -3.1 4,204,039 + BT3286 0.58 +1.3 4,204,268 + BT3286 0.88 -0.6
Or see this region's nucleotide sequence