Experiment: BHIS with Cephalothin 0.625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1982 and BT1983 are separated by 2 nucleotides BT1983 and BT1984 are separated by 33 nucleotides BT1984 and BT1985 overlap by 11 nucleotides BT1985 and BT1986 are separated by 199 nucleotides
BT1982: BT1982 - hypothetical protein (NCBI ptt file), at 2,492,546 to 2,492,875
BT1982
BT1983: BT1983 - hemolysin III (NCBI ptt file), at 2,492,878 to 2,493,567
BT1983
BT1984: BT1984 - transcriptional regulator (NCBI ptt file), at 2,493,601 to 2,493,822
BT1984
BT1985: BT1985 - hypothetical protein (NCBI ptt file), at 2,493,812 to 2,494,270
BT1985
BT1986: BT1986 - putative ribose 5-phosphate isomerase (NCBI ptt file), at 2,494,470 to 2,495,171
BT1986
Position (kb)
2493
2494 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2492.604 kb on + strand, within BT1982 at 2492.607 kb on + strand, within BT1982 at 2492.724 kb on + strand, within BT1982 at 2492.726 kb on + strand, within BT1982 at 2492.727 kb on - strand, within BT1982 at 2492.937 kb on + strand at 2492.937 kb on + strand at 2492.938 kb on - strand at 2492.979 kb on + strand, within BT1983 at 2492.982 kb on - strand, within BT1983 at 2493.062 kb on + strand, within BT1983 at 2493.094 kb on + strand, within BT1983 at 2493.113 kb on - strand, within BT1983 at 2493.171 kb on + strand, within BT1983 at 2493.176 kb on - strand, within BT1983 at 2493.176 kb on - strand, within BT1983 at 2493.196 kb on + strand, within BT1983 at 2493.340 kb on + strand, within BT1983 at 2493.343 kb on - strand, within BT1983 at 2493.344 kb on + strand, within BT1983 at 2493.348 kb on + strand, within BT1983 at 2493.349 kb on - strand, within BT1983 at 2493.349 kb on - strand, within BT1983 at 2493.451 kb on + strand, within BT1983 at 2493.454 kb on - strand, within BT1983 at 2493.473 kb on - strand, within BT1983 at 2493.490 kb on + strand, within BT1983 at 2493.490 kb on + strand, within BT1983 at 2493.492 kb on + strand, within BT1983 at 2493.492 kb on + strand, within BT1983 at 2493.492 kb on + strand, within BT1983 at 2493.493 kb on - strand, within BT1983 at 2493.493 kb on - strand, within BT1983 at 2493.493 kb on - strand, within BT1983 at 2493.493 kb on - strand, within BT1983 at 2493.493 kb on - strand at 2493.534 kb on - strand at 2493.541 kb on - strand at 2493.560 kb on + strand at 2493.560 kb on + strand at 2493.657 kb on - strand, within BT1984 at 2493.665 kb on - strand, within BT1984 at 2493.684 kb on + strand, within BT1984 at 2493.694 kb on - strand, within BT1984 at 2493.794 kb on - strand, within BT1984 at 2493.826 kb on + strand at 2493.827 kb on - strand at 2493.827 kb on - strand at 2493.837 kb on + strand at 2493.855 kb on - strand at 2493.875 kb on + strand, within BT1985 at 2493.876 kb on - strand, within BT1985 at 2493.876 kb on - strand, within BT1985 at 2493.876 kb on - strand, within BT1985 at 2493.901 kb on + strand, within BT1985 at 2493.901 kb on + strand, within BT1985 at 2493.902 kb on - strand, within BT1985 at 2493.941 kb on + strand, within BT1985 at 2493.967 kb on - strand, within BT1985 at 2493.988 kb on + strand, within BT1985 at 2494.008 kb on - strand, within BT1985 at 2494.019 kb on + strand, within BT1985 at 2494.019 kb on + strand, within BT1985 at 2494.019 kb on + strand, within BT1985 at 2494.019 kb on + strand, within BT1985 at 2494.082 kb on + strand, within BT1985 at 2494.093 kb on - strand, within BT1985 at 2494.116 kb on - strand, within BT1985 at 2494.116 kb on - strand, within BT1985 at 2494.116 kb on - strand, within BT1985 at 2494.118 kb on - strand, within BT1985 at 2494.119 kb on + strand, within BT1985 at 2494.120 kb on - strand, within BT1985 at 2494.179 kb on - strand, within BT1985 at 2494.182 kb on - strand, within BT1985 at 2494.238 kb on - strand at 2494.259 kb on - strand at 2494.363 kb on + strand at 2494.660 kb on + strand, within BT1986 at 2494.660 kb on + strand, within BT1986 at 2494.708 kb on + strand, within BT1986 at 2494.709 kb on - strand, within BT1986 at 2494.709 kb on - strand, within BT1986 at 2494.781 kb on - strand, within BT1986
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Cephalothin 0.625 mg/ml remove 2,492,604 + BT1982 0.18 +1.1 2,492,607 + BT1982 0.18 +0.3 2,492,724 + BT1982 0.54 -0.9 2,492,726 + BT1982 0.55 -0.8 2,492,727 - BT1982 0.55 -1.7 2,492,937 + -0.7 2,492,937 + -1.6 2,492,938 - -1.7 2,492,979 + BT1983 0.15 -1.7 2,492,982 - BT1983 0.15 -1.3 2,493,062 + BT1983 0.27 -1.1 2,493,094 + BT1983 0.31 +1.1 2,493,113 - BT1983 0.34 +0.2 2,493,171 + BT1983 0.42 -0.9 2,493,176 - BT1983 0.43 +1.1 2,493,176 - BT1983 0.43 +0.5 2,493,196 + BT1983 0.46 +2.1 2,493,340 + BT1983 0.67 -1.9 2,493,343 - BT1983 0.67 -2.3 2,493,344 + BT1983 0.68 +0.9 2,493,348 + BT1983 0.68 +0.1 2,493,349 - BT1983 0.68 -0.9 2,493,349 - BT1983 0.68 -1.0 2,493,451 + BT1983 0.83 -0.7 2,493,454 - BT1983 0.83 +2.6 2,493,473 - BT1983 0.86 +0.2 2,493,490 + BT1983 0.89 +0.1 2,493,490 + BT1983 0.89 -3.0 2,493,492 + BT1983 0.89 -2.4 2,493,492 + BT1983 0.89 +1.1 2,493,492 + BT1983 0.89 -0.4 2,493,493 - BT1983 0.89 +0.6 2,493,493 - BT1983 0.89 +0.5 2,493,493 - BT1983 0.89 -1.5 2,493,493 - BT1983 0.89 +0.2 2,493,493 - +1.1 2,493,534 - -0.5 2,493,541 - -0.3 2,493,560 + +0.3 2,493,560 + +0.1 2,493,657 - BT1984 0.25 -2.1 2,493,665 - BT1984 0.29 -1.3 2,493,684 + BT1984 0.37 -2.6 2,493,694 - BT1984 0.42 -1.8 2,493,794 - BT1984 0.87 -2.8 2,493,826 + -0.8 2,493,827 - -3.5 2,493,827 - -2.9 2,493,837 + -1.3 2,493,855 - -0.0 2,493,875 + BT1985 0.14 -2.3 2,493,876 - BT1985 0.14 +1.3 2,493,876 - BT1985 0.14 +2.5 2,493,876 - BT1985 0.14 +0.1 2,493,901 + BT1985 0.19 -1.1 2,493,901 + BT1985 0.19 -0.2 2,493,902 - BT1985 0.20 -0.6 2,493,941 + BT1985 0.28 -0.5 2,493,967 - BT1985 0.34 -0.1 2,493,988 + BT1985 0.38 -0.1 2,494,008 - BT1985 0.43 +0.5 2,494,019 + BT1985 0.45 +0.6 2,494,019 + BT1985 0.45 +0.1 2,494,019 + BT1985 0.45 -0.2 2,494,019 + BT1985 0.45 -1.3 2,494,082 + BT1985 0.59 +0.4 2,494,093 - BT1985 0.61 -1.2 2,494,116 - BT1985 0.66 -1.8 2,494,116 - BT1985 0.66 -0.7 2,494,116 - BT1985 0.66 -0.5 2,494,118 - BT1985 0.67 -0.8 2,494,119 + BT1985 0.67 -0.5 2,494,120 - BT1985 0.67 -0.9 2,494,179 - BT1985 0.80 -0.5 2,494,182 - BT1985 0.81 -2.5 2,494,238 - -0.3 2,494,259 - -0.0 2,494,363 + -0.3 2,494,660 + BT1986 0.27 +0.5 2,494,660 + BT1986 0.27 -1.3 2,494,708 + BT1986 0.34 +1.0 2,494,709 - BT1986 0.34 -0.4 2,494,709 - BT1986 0.34 -0.1 2,494,781 - BT1986 0.44 -0.3
Or see this region's nucleotide sequence