Experiment: BHIS with Cephalothin 0.625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1694 and BT1695 are separated by 42 nucleotides BT1695 and BT1696 are separated by 115 nucleotides
BT1694: BT1694 - transporter, AcrB/D/F family (NCBI ptt file), at 2,089,985 to 2,093,023
BT1694
BT1695: BT1695 - outer membrane efflux protein (NCBI ptt file), at 2,093,066 to 2,094,346
BT1695
BT1696: BT1696 - oxaloacetate decarboxylase beta chain (NCBI ptt file), at 2,094,462 to 2,095,697
BT1696
Position (kb)
2093
2094
2095 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2092.140 kb on + strand, within BT1694 at 2092.229 kb on - strand, within BT1694 at 2092.379 kb on - strand, within BT1694 at 2092.453 kb on - strand, within BT1694 at 2092.614 kb on - strand, within BT1694 at 2092.614 kb on - strand, within BT1694 at 2092.725 kb on + strand at 2092.761 kb on + strand at 2092.948 kb on - strand at 2092.948 kb on - strand at 2092.969 kb on - strand at 2093.033 kb on - strand at 2093.039 kb on + strand at 2093.091 kb on + strand at 2093.191 kb on - strand at 2093.209 kb on - strand, within BT1695 at 2093.351 kb on + strand, within BT1695 at 2093.385 kb on - strand, within BT1695 at 2093.418 kb on + strand, within BT1695 at 2093.498 kb on + strand, within BT1695 at 2093.529 kb on + strand, within BT1695 at 2093.570 kb on + strand, within BT1695 at 2093.665 kb on + strand, within BT1695 at 2093.753 kb on + strand, within BT1695 at 2093.901 kb on + strand, within BT1695 at 2093.902 kb on - strand, within BT1695 at 2093.959 kb on - strand, within BT1695 at 2093.959 kb on - strand, within BT1695 at 2093.993 kb on - strand, within BT1695 at 2094.047 kb on + strand, within BT1695 at 2094.058 kb on - strand, within BT1695 at 2094.077 kb on + strand, within BT1695 at 2094.079 kb on + strand, within BT1695 at 2094.079 kb on + strand, within BT1695 at 2094.091 kb on + strand, within BT1695 at 2094.324 kb on - strand at 2094.345 kb on - strand at 2094.349 kb on - strand at 2094.391 kb on - strand at 2094.463 kb on + strand at 2094.524 kb on + strand at 2094.525 kb on - strand at 2094.533 kb on + strand at 2094.534 kb on - strand at 2094.538 kb on + strand at 2094.642 kb on - strand, within BT1696 at 2094.699 kb on - strand, within BT1696 at 2094.806 kb on + strand, within BT1696 at 2094.806 kb on + strand, within BT1696 at 2094.807 kb on - strand, within BT1696 at 2094.821 kb on + strand, within BT1696 at 2094.881 kb on + strand, within BT1696 at 2094.910 kb on - strand, within BT1696 at 2094.934 kb on - strand, within BT1696 at 2094.948 kb on + strand, within BT1696 at 2094.973 kb on - strand, within BT1696 at 2095.007 kb on - strand, within BT1696 at 2095.049 kb on + strand, within BT1696 at 2095.049 kb on + strand, within BT1696 at 2095.050 kb on - strand, within BT1696 at 2095.052 kb on + strand, within BT1696 at 2095.100 kb on + strand, within BT1696 at 2095.101 kb on - strand, within BT1696 at 2095.102 kb on + strand, within BT1696 at 2095.102 kb on + strand, within BT1696 at 2095.103 kb on - strand, within BT1696 at 2095.103 kb on - strand, within BT1696 at 2095.266 kb on + strand, within BT1696
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Cephalothin 0.625 mg/ml remove 2,092,140 + BT1694 0.71 -0.8 2,092,229 - BT1694 0.74 -2.5 2,092,379 - BT1694 0.79 +0.3 2,092,453 - BT1694 0.81 +1.0 2,092,614 - BT1694 0.87 -2.5 2,092,614 - BT1694 0.87 +0.4 2,092,725 + -1.4 2,092,761 + -3.3 2,092,948 - +0.1 2,092,948 - -0.3 2,092,969 - -0.0 2,093,033 - -1.0 2,093,039 + +0.5 2,093,091 + -1.8 2,093,191 - +1.0 2,093,209 - BT1695 0.11 +0.6 2,093,351 + BT1695 0.22 -0.7 2,093,385 - BT1695 0.25 -2.2 2,093,418 + BT1695 0.27 -0.5 2,093,498 + BT1695 0.34 -1.3 2,093,529 + BT1695 0.36 -1.0 2,093,570 + BT1695 0.39 -1.7 2,093,665 + BT1695 0.47 -1.6 2,093,753 + BT1695 0.54 -0.4 2,093,901 + BT1695 0.65 -1.5 2,093,902 - BT1695 0.65 -2.3 2,093,959 - BT1695 0.70 -1.8 2,093,959 - BT1695 0.70 -2.4 2,093,993 - BT1695 0.72 -0.8 2,094,047 + BT1695 0.77 -2.2 2,094,058 - BT1695 0.77 -0.3 2,094,077 + BT1695 0.79 -1.5 2,094,079 + BT1695 0.79 -0.0 2,094,079 + BT1695 0.79 -1.3 2,094,091 + BT1695 0.80 -0.8 2,094,324 - -1.2 2,094,345 - +0.4 2,094,349 - -2.0 2,094,391 - -1.0 2,094,463 + -1.1 2,094,524 + +0.3 2,094,525 - -1.1 2,094,533 + +0.4 2,094,534 - -0.0 2,094,538 + -0.0 2,094,642 - BT1696 0.15 -0.0 2,094,699 - BT1696 0.19 -1.4 2,094,806 + BT1696 0.28 -0.7 2,094,806 + BT1696 0.28 -1.8 2,094,807 - BT1696 0.28 -2.5 2,094,821 + BT1696 0.29 -0.7 2,094,881 + BT1696 0.34 -1.2 2,094,910 - BT1696 0.36 -0.8 2,094,934 - BT1696 0.38 -0.2 2,094,948 + BT1696 0.39 -0.8 2,094,973 - BT1696 0.41 -0.0 2,095,007 - BT1696 0.44 +1.8 2,095,049 + BT1696 0.47 +2.0 2,095,049 + BT1696 0.47 +0.5 2,095,050 - BT1696 0.48 -0.7 2,095,052 + BT1696 0.48 +0.2 2,095,100 + BT1696 0.52 -0.0 2,095,101 - BT1696 0.52 -2.5 2,095,102 + BT1696 0.52 -1.8 2,095,102 + BT1696 0.52 -2.4 2,095,103 - BT1696 0.52 -0.4 2,095,103 - BT1696 0.52 -2.6 2,095,266 + BT1696 0.65 -0.7
Or see this region's nucleotide sequence