Experiment: BHIS with Cephalothin 0.625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1007 and BT1008 are separated by 66 nucleotides BT1008 and BT1009 are separated by 5 nucleotides
BT1007: BT1007 - conserved hypothetical protein (NCBI ptt file), at 1,254,189 to 1,254,980
BT1007
BT1008: BT1008 - xylanase (NCBI ptt file), at 1,255,047 to 1,255,835
BT1008
BT1009: BT1009 - dihydroorotate dehydrogenase (NCBI ptt file), at 1,255,841 to 1,256,779
BT1009
Position (kb)
1255
1256 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1254.060 kb on + strand at 1254.078 kb on - strand at 1254.092 kb on + strand at 1254.152 kb on + strand at 1254.191 kb on - strand at 1254.228 kb on + strand at 1254.248 kb on + strand at 1254.257 kb on + strand at 1254.302 kb on - strand, within BT1007 at 1254.306 kb on + strand, within BT1007 at 1254.307 kb on - strand, within BT1007 at 1254.310 kb on - strand, within BT1007 at 1254.332 kb on + strand, within BT1007 at 1254.345 kb on - strand, within BT1007 at 1254.368 kb on + strand, within BT1007 at 1254.369 kb on - strand, within BT1007 at 1254.420 kb on - strand, within BT1007 at 1254.424 kb on + strand, within BT1007 at 1254.427 kb on - strand, within BT1007 at 1254.516 kb on + strand, within BT1007 at 1254.517 kb on - strand, within BT1007 at 1254.525 kb on - strand, within BT1007 at 1254.607 kb on + strand, within BT1007 at 1254.609 kb on + strand, within BT1007 at 1254.630 kb on - strand, within BT1007 at 1254.630 kb on - strand, within BT1007 at 1254.634 kb on - strand, within BT1007 at 1254.778 kb on - strand, within BT1007 at 1254.780 kb on - strand, within BT1007 at 1254.803 kb on + strand, within BT1007 at 1254.919 kb on - strand at 1254.956 kb on + strand at 1254.956 kb on + strand at 1255.013 kb on + strand at 1255.013 kb on + strand at 1255.022 kb on + strand at 1255.097 kb on - strand at 1255.164 kb on - strand, within BT1008 at 1255.193 kb on + strand, within BT1008 at 1255.194 kb on - strand, within BT1008 at 1255.237 kb on - strand, within BT1008 at 1255.268 kb on + strand, within BT1008 at 1255.285 kb on + strand, within BT1008 at 1255.309 kb on + strand, within BT1008 at 1255.585 kb on + strand, within BT1008 at 1255.586 kb on - strand, within BT1008 at 1255.586 kb on - strand, within BT1008 at 1255.589 kb on + strand, within BT1008 at 1255.590 kb on - strand, within BT1008 at 1255.674 kb on - strand, within BT1008 at 1255.783 kb on + strand at 1255.784 kb on - strand at 1255.807 kb on + strand at 1255.815 kb on - strand at 1255.818 kb on - strand at 1255.895 kb on - strand at 1256.016 kb on + strand, within BT1009 at 1256.016 kb on + strand, within BT1009 at 1256.333 kb on + strand, within BT1009 at 1256.334 kb on - strand, within BT1009 at 1256.468 kb on + strand, within BT1009 at 1256.562 kb on + strand, within BT1009 at 1256.754 kb on + strand at 1256.787 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Cephalothin 0.625 mg/ml remove 1,254,060 + -2.2 1,254,078 - +1.2 1,254,092 + -1.5 1,254,152 + -1.0 1,254,191 - +0.4 1,254,228 + +0.6 1,254,248 + +0.4 1,254,257 + -0.3 1,254,302 - BT1007 0.14 +0.1 1,254,306 + BT1007 0.15 -1.4 1,254,307 - BT1007 0.15 -2.1 1,254,310 - BT1007 0.15 -0.6 1,254,332 + BT1007 0.18 -1.3 1,254,345 - BT1007 0.20 +2.0 1,254,368 + BT1007 0.23 -3.5 1,254,369 - BT1007 0.23 -0.5 1,254,420 - BT1007 0.29 -0.6 1,254,424 + BT1007 0.30 -0.8 1,254,427 - BT1007 0.30 -2.1 1,254,516 + BT1007 0.41 +0.7 1,254,517 - BT1007 0.41 +0.4 1,254,525 - BT1007 0.42 -0.7 1,254,607 + BT1007 0.53 +1.5 1,254,609 + BT1007 0.53 -1.8 1,254,630 - BT1007 0.56 -0.8 1,254,630 - BT1007 0.56 +0.1 1,254,634 - BT1007 0.56 -0.5 1,254,778 - BT1007 0.74 +0.1 1,254,780 - BT1007 0.75 -1.3 1,254,803 + BT1007 0.78 -2.4 1,254,919 - -2.4 1,254,956 + +1.3 1,254,956 + -2.1 1,255,013 + +0.7 1,255,013 + -0.2 1,255,022 + -3.1 1,255,097 - -1.3 1,255,164 - BT1008 0.15 -2.1 1,255,193 + BT1008 0.19 -0.4 1,255,194 - BT1008 0.19 -1.4 1,255,237 - BT1008 0.24 -0.2 1,255,268 + BT1008 0.28 -0.5 1,255,285 + BT1008 0.30 -0.6 1,255,309 + BT1008 0.33 -1.8 1,255,585 + BT1008 0.68 -2.4 1,255,586 - BT1008 0.68 -1.6 1,255,586 - BT1008 0.68 -3.4 1,255,589 + BT1008 0.69 -0.9 1,255,590 - BT1008 0.69 -1.3 1,255,674 - BT1008 0.79 -2.4 1,255,783 + -0.3 1,255,784 - -2.7 1,255,807 + -0.5 1,255,815 - +0.1 1,255,818 - +0.5 1,255,895 - +0.2 1,256,016 + BT1009 0.19 +0.0 1,256,016 + BT1009 0.19 -0.1 1,256,333 + BT1009 0.52 +2.1 1,256,334 - BT1009 0.53 -0.8 1,256,468 + BT1009 0.67 -2.4 1,256,562 + BT1009 0.77 -2.7 1,256,754 + +1.3 1,256,787 - -0.3
Or see this region's nucleotide sequence