Experiment: BHIS with Cephalothin 0.625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0685 and BT0686 are separated by 168 nucleotides BT0686 and BT_0687 are separated by 147 nucleotides
BT0685: BT0685 - putative Na+/H+ exchanger (NCBI ptt file), at 851,405 to 852,877
BT0685
BT0686: BT0686 - putative uracil permease (uracil transporter) (NCBI ptt file), at 853,046 to 854,230
BT0686
BT_0687: BT_0687 - hydroxylamine reductase (RefSeq), at 854,378 to 856,003
_0687
Position (kb)
853
854
855 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 852.055 kb on - strand, within BT0685 at 852.098 kb on - strand, within BT0685 at 852.185 kb on - strand at 852.185 kb on - strand, within BT0685 at 852.360 kb on + strand, within BT0685 at 852.517 kb on - strand, within BT0685 at 852.558 kb on - strand, within BT0685 at 852.582 kb on - strand, within BT0685 at 852.617 kb on + strand, within BT0685 at 852.617 kb on + strand, within BT0685 at 852.652 kb on + strand, within BT0685 at 852.653 kb on - strand, within BT0685 at 852.689 kb on - strand, within BT0685 at 852.774 kb on - strand at 852.848 kb on + strand at 852.885 kb on - strand at 852.890 kb on - strand at 853.041 kb on + strand at 853.140 kb on + strand at 853.187 kb on - strand, within BT0686 at 853.187 kb on - strand, within BT0686 at 853.267 kb on - strand, within BT0686 at 853.402 kb on + strand, within BT0686 at 853.418 kb on - strand, within BT0686 at 853.457 kb on - strand, within BT0686 at 853.549 kb on + strand, within BT0686 at 853.735 kb on - strand, within BT0686 at 853.737 kb on - strand, within BT0686 at 853.737 kb on - strand, within BT0686 at 853.753 kb on + strand, within BT0686 at 853.859 kb on + strand, within BT0686 at 853.936 kb on + strand at 853.937 kb on - strand, within BT0686 at 853.941 kb on + strand, within BT0686 at 854.047 kb on - strand, within BT0686 at 854.051 kb on - strand, within BT0686 at 854.051 kb on - strand, within BT0686 at 854.068 kb on + strand, within BT0686 at 854.068 kb on + strand, within BT0686 at 854.196 kb on + strand at 854.211 kb on + strand at 854.316 kb on + strand at 854.317 kb on - strand at 854.345 kb on - strand at 854.356 kb on - strand at 854.362 kb on + strand at 854.366 kb on + strand at 854.367 kb on - strand at 854.447 kb on + strand at 854.486 kb on + strand at 854.500 kb on + strand at 854.501 kb on - strand at 854.556 kb on + strand, within BT_0687 at 854.557 kb on - strand, within BT_0687 at 854.573 kb on + strand, within BT_0687 at 854.574 kb on - strand, within BT_0687 at 854.657 kb on - strand, within BT_0687 at 854.725 kb on - strand, within BT_0687 at 854.787 kb on + strand, within BT_0687 at 854.807 kb on - strand, within BT_0687 at 854.868 kb on - strand, within BT_0687 at 854.891 kb on + strand, within BT_0687 at 854.892 kb on - strand, within BT_0687 at 855.059 kb on - strand, within BT_0687 at 855.070 kb on - strand, within BT_0687
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Cephalothin 0.625 mg/ml remove 852,055 - BT0685 0.44 +0.2 852,098 - BT0685 0.47 +0.8 852,185 - +0.8 852,185 - BT0685 0.53 -1.8 852,360 + BT0685 0.65 +0.6 852,517 - BT0685 0.75 -1.4 852,558 - BT0685 0.78 +0.8 852,582 - BT0685 0.80 -1.2 852,617 + BT0685 0.82 +1.9 852,617 + BT0685 0.82 -1.4 852,652 + BT0685 0.85 -1.6 852,653 - BT0685 0.85 -1.8 852,689 - BT0685 0.87 -0.1 852,774 - -2.1 852,848 + -0.2 852,885 - -1.7 852,890 - -2.4 853,041 + -0.8 853,140 + +0.8 853,187 - BT0686 0.12 +0.5 853,187 - BT0686 0.12 -2.1 853,267 - BT0686 0.19 -0.2 853,402 + BT0686 0.30 -0.7 853,418 - BT0686 0.31 +1.8 853,457 - BT0686 0.35 -2.1 853,549 + BT0686 0.42 -0.6 853,735 - BT0686 0.58 -2.2 853,737 - BT0686 0.58 -0.2 853,737 - BT0686 0.58 -0.8 853,753 + BT0686 0.60 -1.0 853,859 + BT0686 0.69 -1.2 853,936 + -0.2 853,937 - BT0686 0.75 -1.8 853,941 + BT0686 0.76 -0.1 854,047 - BT0686 0.84 -0.1 854,051 - BT0686 0.85 +1.2 854,051 - BT0686 0.85 -1.1 854,068 + BT0686 0.86 -0.6 854,068 + BT0686 0.86 -1.2 854,196 + -1.2 854,211 + -0.6 854,316 + +0.5 854,317 - +1.8 854,345 - +0.9 854,356 - -0.4 854,362 + -0.1 854,366 + -2.5 854,367 - +2.6 854,447 + +0.4 854,486 + -0.8 854,500 + -0.6 854,501 - +0.8 854,556 + BT_0687 0.11 +0.1 854,557 - BT_0687 0.11 -1.2 854,573 + BT_0687 0.12 -0.5 854,574 - BT_0687 0.12 -0.2 854,657 - BT_0687 0.17 -0.2 854,725 - BT_0687 0.21 +0.9 854,787 + BT_0687 0.25 -1.7 854,807 - BT_0687 0.26 +3.3 854,868 - BT_0687 0.30 +0.2 854,891 + BT_0687 0.32 -1.2 854,892 - BT_0687 0.32 -0.5 855,059 - BT_0687 0.42 -1.8 855,070 - BT_0687 0.43 -1.0
Or see this region's nucleotide sequence