Experiment: MoLS4 with L-Serine (N), without W
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lys1 and nspC are separated by 277 nucleotides nspC and DORF27889 are separated by 41 nucleotides DORF27889 and DVU0420 are separated by 51 nucleotides DVU0420 and DVU0421 are separated by 10 nucleotides
DVU0418: lys1 - saccharopine dehydrogenase (TIGR), at 470,885 to 472,075
lys1
DVU0419: nspC - carboxynorspermidine decarboxylase (TIGR), at 472,353 to 473,525
nspC
DORF27889: DORF27889 - no description, at 473,567 to 473,728
DORF27889
DVU0420: DVU0420 - hypothetical protein (TIGR), at 473,780 to 473,956
DVU0420
DVU0421: DVU0421 - agmatinase, putative (TIGR), at 473,967 to 474,839
DVU0421
Position (kb) 472
473
474 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 471.504 kb on - strand, within lys1 at 471.584 kb on + strand, within lys1 at 471.657 kb on + strand, within lys1 at 471.666 kb on + strand, within lys1 at 471.666 kb on + strand, within lys1 at 471.666 kb on + strand, within lys1 at 471.674 kb on - strand, within lys1 at 471.710 kb on + strand, within lys1 at 471.740 kb on + strand, within lys1 at 471.816 kb on + strand, within lys1 at 471.852 kb on - strand, within lys1 at 471.932 kb on - strand, within lys1 at 471.945 kb on - strand, within lys1 at 471.980 kb on + strand at 472.004 kb on - strand at 472.033 kb on + strand at 472.051 kb on + strand at 472.142 kb on - strand at 472.152 kb on - strand at 472.160 kb on - strand at 472.167 kb on + strand at 472.175 kb on - strand at 472.292 kb on + strand at 472.292 kb on + strand at 472.292 kb on + strand at 472.300 kb on - strand at 472.300 kb on - strand at 472.335 kb on + strand at 472.402 kb on - strand at 472.432 kb on - strand at 472.439 kb on - strand at 472.461 kb on + strand at 472.461 kb on + strand at 472.461 kb on + strand at 472.469 kb on - strand at 472.537 kb on + strand, within nspC at 472.537 kb on + strand, within nspC at 472.538 kb on - strand, within nspC at 472.562 kb on - strand, within nspC at 472.562 kb on - strand, within nspC at 472.603 kb on - strand, within nspC at 472.638 kb on + strand, within nspC at 472.712 kb on + strand, within nspC at 472.791 kb on + strand, within nspC at 472.897 kb on + strand, within nspC at 472.905 kb on - strand, within nspC at 472.959 kb on + strand, within nspC at 473.043 kb on - strand, within nspC at 473.056 kb on - strand, within nspC at 473.064 kb on - strand, within nspC at 473.093 kb on - strand, within nspC at 473.098 kb on - strand, within nspC at 473.138 kb on + strand, within nspC at 473.160 kb on + strand, within nspC at 473.195 kb on - strand, within nspC at 473.207 kb on + strand, within nspC at 473.262 kb on - strand, within nspC at 473.317 kb on + strand, within nspC at 473.450 kb on + strand at 473.453 kb on - strand at 473.480 kb on - strand at 473.526 kb on - strand at 473.527 kb on - strand at 473.527 kb on - strand at 473.633 kb on - strand, within DORF27889 at 473.662 kb on + strand, within DORF27889 at 473.694 kb on + strand, within DORF27889 at 473.708 kb on - strand, within DORF27889 at 473.739 kb on + strand at 473.794 kb on + strand at 473.833 kb on + strand, within DVU0420 at 473.884 kb on + strand, within DVU0420 at 473.960 kb on - strand at 474.034 kb on + strand at 474.035 kb on + strand at 474.118 kb on + strand, within DVU0421 at 474.126 kb on - strand, within DVU0421 at 474.144 kb on + strand, within DVU0421 at 474.186 kb on + strand, within DVU0421 at 474.213 kb on - strand, within DVU0421 at 474.271 kb on - strand, within DVU0421 at 474.294 kb on + strand, within DVU0421 at 474.385 kb on + strand, within DVU0421 at 474.413 kb on - strand, within DVU0421 at 474.422 kb on - strand, within DVU0421 at 474.489 kb on - strand, within DVU0421 at 474.514 kb on + strand, within DVU0421
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Serine (N), without W remove 471,504 - lys1 DVU0418 0.52 -0.8 471,584 + lys1 DVU0418 0.59 -0.8 471,657 + lys1 DVU0418 0.65 -0.4 471,666 + lys1 DVU0418 0.66 -1.7 471,666 + lys1 DVU0418 0.66 -1.6 471,666 + lys1 DVU0418 0.66 -1.6 471,674 - lys1 DVU0418 0.66 -2.0 471,710 + lys1 DVU0418 0.69 -0.6 471,740 + lys1 DVU0418 0.72 -0.9 471,816 + lys1 DVU0418 0.78 -0.2 471,852 - lys1 DVU0418 0.81 -1.2 471,932 - lys1 DVU0418 0.88 -2.3 471,945 - lys1 DVU0418 0.89 -2.3 471,980 + -2.7 472,004 - -1.5 472,033 + +1.1 472,051 + -0.8 472,142 - -0.8 472,152 - -1.5 472,160 - -0.4 472,167 + -0.2 472,175 - -2.0 472,292 + -0.9 472,292 + -0.0 472,292 + -0.2 472,300 - +0.7 472,300 - +0.1 472,335 + +0.0 472,402 - -1.1 472,432 - -0.7 472,439 - -1.3 472,461 + -1.6 472,461 + +0.6 472,461 + -1.3 472,469 - -1.3 472,537 + nspC DVU0419 0.16 -0.3 472,537 + nspC DVU0419 0.16 -1.0 472,538 - nspC DVU0419 0.16 -1.8 472,562 - nspC DVU0419 0.18 -1.3 472,562 - nspC DVU0419 0.18 -1.5 472,603 - nspC DVU0419 0.21 -1.0 472,638 + nspC DVU0419 0.24 -0.3 472,712 + nspC DVU0419 0.31 -1.0 472,791 + nspC DVU0419 0.37 +0.1 472,897 + nspC DVU0419 0.46 -1.0 472,905 - nspC DVU0419 0.47 -0.3 472,959 + nspC DVU0419 0.52 -0.7 473,043 - nspC DVU0419 0.59 -0.6 473,056 - nspC DVU0419 0.60 -3.3 473,064 - nspC DVU0419 0.61 -0.9 473,093 - nspC DVU0419 0.63 -1.1 473,098 - nspC DVU0419 0.64 +0.2 473,138 + nspC DVU0419 0.67 -1.2 473,160 + nspC DVU0419 0.69 -0.8 473,195 - nspC DVU0419 0.72 -1.4 473,207 + nspC DVU0419 0.73 -2.9 473,262 - nspC DVU0419 0.77 +1.1 473,317 + nspC DVU0419 0.82 +0.1 473,450 + -0.1 473,453 - -1.8 473,480 - -0.2 473,526 - +0.4 473,527 - -0.4 473,527 - -0.6 473,633 - DORF27889 0.41 -1.8 473,662 + DORF27889 0.59 +0.2 473,694 + DORF27889 0.78 -0.3 473,708 - DORF27889 0.87 -0.0 473,739 + -0.3 473,794 + -0.1 473,833 + DVU0420 0.30 +0.3 473,884 + DVU0420 0.59 -0.9 473,960 - -0.7 474,034 + -1.0 474,035 + -0.1 474,118 + DVU0421 0.17 -0.4 474,126 - DVU0421 0.18 -1.8 474,144 + DVU0421 0.20 -1.9 474,186 + DVU0421 0.25 -0.9 474,213 - DVU0421 0.28 -1.0 474,271 - DVU0421 0.35 +0.5 474,294 + DVU0421 0.37 -0.4 474,385 + DVU0421 0.48 -0.1 474,413 - DVU0421 0.51 +1.3 474,422 - DVU0421 0.52 -1.5 474,489 - DVU0421 0.60 +0.9 474,514 + DVU0421 0.63 -1.2
Or see this region's nucleotide sequence