Experiment: MoLS4 with L-Serine (N), without W
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tRNA-Val and tRNA-Asp are separated by 3 nucleotides tRNA-Asp and tRNA-Asp are separated by 71 nucleotides tRNA-Asp and DVU0276 are separated by 265 nucleotides DVU0276 and DORF26828 are separated by 147 nucleotides DORF26828 and DVU0277 are separated by 176 nucleotides
DVU_tRNA-Val-1: tRNA-Val - tRNA with anticodon CAC for Val (VIMSS-AUTO), at 318,835 to 318,910
tRNA-Val
DVU_tRNA-Asp-1: tRNA-Asp - tRNA with anticodon GTC for Asp (VIMSS-AUTO), at 318,914 to 318,990
tRNA-Asp
DVU_tRNA-Asp-2: tRNA-Asp - tRNA with anticodon GTC for Asp (VIMSS-AUTO), at 319,062 to 319,138
tRNA-Asp
DVU0276: DVU0276 - conserved hypothetical protein (TIGR), at 319,404 to 319,802
DVU0276
DORF26828: DORF26828 - SEED: FIG00605560: hypothetical protein, at 319,950 to 320,180
DORF26828
DVU0277: DVU0277 - transcriptional regulator, AraC family (TIGR), at 320,357 to 321,337
DVU0277
Position (kb) 319
320 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 318.424 kb on + strand at 318.462 kb on - strand at 318.585 kb on + strand at 318.585 kb on + strand at 318.593 kb on - strand at 318.667 kb on + strand at 318.667 kb on + strand at 318.677 kb on + strand at 318.685 kb on - strand at 318.692 kb on + strand at 318.779 kb on + strand at 319.161 kb on + strand at 319.265 kb on + strand at 319.364 kb on + strand at 319.394 kb on - strand at 319.401 kb on + strand at 319.411 kb on + strand at 319.411 kb on + strand at 319.419 kb on - strand at 319.458 kb on - strand, within DVU0276 at 319.474 kb on - strand, within DVU0276 at 319.476 kb on + strand, within DVU0276 at 319.553 kb on + strand, within DVU0276 at 319.563 kb on + strand, within DVU0276 at 319.568 kb on + strand, within DVU0276 at 319.583 kb on - strand, within DVU0276 at 319.587 kb on + strand, within DVU0276 at 319.595 kb on - strand, within DVU0276 at 319.605 kb on - strand, within DVU0276 at 319.619 kb on + strand, within DVU0276 at 319.640 kb on - strand, within DVU0276 at 319.658 kb on + strand, within DVU0276 at 319.658 kb on + strand, within DVU0276 at 319.697 kb on - strand, within DVU0276 at 319.711 kb on + strand, within DVU0276 at 319.715 kb on + strand, within DVU0276 at 319.723 kb on - strand, within DVU0276 at 319.723 kb on - strand, within DVU0276 at 319.734 kb on + strand, within DVU0276 at 319.734 kb on + strand, within DVU0276 at 319.743 kb on - strand, within DVU0276 at 319.779 kb on + strand at 319.787 kb on - strand at 319.787 kb on - strand at 319.789 kb on - strand at 319.843 kb on + strand at 319.843 kb on + strand at 319.848 kb on + strand at 319.856 kb on - strand at 319.856 kb on - strand at 320.024 kb on - strand, within DORF26828 at 320.026 kb on + strand, within DORF26828 at 320.034 kb on - strand, within DORF26828 at 320.051 kb on + strand, within DORF26828 at 320.115 kb on - strand, within DORF26828 at 320.209 kb on - strand at 320.245 kb on - strand at 320.245 kb on - strand at 320.304 kb on - strand at 320.341 kb on + strand at 320.346 kb on - strand at 320.375 kb on - strand at 320.465 kb on + strand, within DVU0277 at 320.505 kb on + strand, within DVU0277 at 320.516 kb on + strand, within DVU0277 at 320.524 kb on - strand, within DVU0277 at 320.524 kb on - strand, within DVU0277 at 320.711 kb on - strand, within DVU0277 at 320.719 kb on + strand, within DVU0277 at 320.722 kb on + strand, within DVU0277 at 320.780 kb on + strand, within DVU0277 at 320.783 kb on - strand, within DVU0277
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Serine (N), without W remove 318,424 + -1.4 318,462 - -3.3 318,585 + -0.5 318,585 + -0.9 318,593 - -1.1 318,667 + -0.6 318,667 + -0.9 318,677 + -0.2 318,685 - -0.9 318,692 + +0.0 318,779 + +0.3 319,161 + +0.0 319,265 + -0.4 319,364 + -0.1 319,394 - -0.1 319,401 + +0.7 319,411 + -0.3 319,411 + -0.2 319,419 - -0.4 319,458 - DVU0276 0.14 +0.2 319,474 - DVU0276 0.18 +0.5 319,476 + DVU0276 0.18 -1.8 319,553 + DVU0276 0.37 -0.3 319,563 + DVU0276 0.40 -0.7 319,568 + DVU0276 0.41 -1.1 319,583 - DVU0276 0.45 -0.3 319,587 + DVU0276 0.46 -1.2 319,595 - DVU0276 0.48 -1.3 319,605 - DVU0276 0.50 -0.2 319,619 + DVU0276 0.54 +0.6 319,640 - DVU0276 0.59 -1.0 319,658 + DVU0276 0.64 -0.3 319,658 + DVU0276 0.64 -0.0 319,697 - DVU0276 0.73 -0.6 319,711 + DVU0276 0.77 -0.4 319,715 + DVU0276 0.78 -0.0 319,723 - DVU0276 0.80 -1.2 319,723 - DVU0276 0.80 -1.0 319,734 + DVU0276 0.83 -0.7 319,734 + DVU0276 0.83 -0.7 319,743 - DVU0276 0.85 +0.4 319,779 + -0.5 319,787 - +1.5 319,787 - -2.1 319,789 - -0.4 319,843 + -0.0 319,843 + -1.4 319,848 + -0.3 319,856 - -0.4 319,856 - -0.7 320,024 - DORF26828 0.32 -0.7 320,026 + DORF26828 0.33 +0.2 320,034 - DORF26828 0.36 -0.1 320,051 + DORF26828 0.44 -4.2 320,115 - DORF26828 0.71 -0.9 320,209 - -1.5 320,245 - -0.2 320,245 - -0.1 320,304 - -0.9 320,341 + -0.3 320,346 - -0.3 320,375 - +0.8 320,465 + DVU0277 0.11 -0.4 320,505 + DVU0277 0.15 -0.5 320,516 + DVU0277 0.16 +1.0 320,524 - DVU0277 0.17 -0.5 320,524 - DVU0277 0.17 -0.7 320,711 - DVU0277 0.36 +0.2 320,719 + DVU0277 0.37 -0.7 320,722 + DVU0277 0.37 +1.0 320,780 + DVU0277 0.43 +1.5 320,783 - DVU0277 0.43 +2.7
Or see this region's nucleotide sequence