Experiment: H2 N-free with 10mM D-glucosamine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03440 and MMJJ_RS03445 are separated by 745 nucleotides MMJJ_RS03445 and MMJJ_RS03450 are separated by 50 nucleotides MMJJ_RS03450 and MMJJ_RS03455 are separated by 78 nucleotides
MMJJ_RS03440: MMJJ_RS03440 - hypothetical protein, at 645,607 to 645,873
_RS03440
MMJJ_RS03445: MMJJ_RS03445 - hypothetical protein, at 646,619 to 647,167
_RS03445
MMJJ_RS03450: MMJJ_RS03450 - hypothetical protein, at 647,218 to 647,619
_RS03450
MMJJ_RS03455: MMJJ_RS03455 - DUF4868 domain-containing protein, at 647,698 to 648,720
_RS03455
Position (kb) 646
647
648 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 645.627 kb on - strand at 645.645 kb on + strand, within MMJJ_RS03440 at 645.646 kb on + strand, within MMJJ_RS03440 at 645.646 kb on + strand, within MMJJ_RS03440 at 645.648 kb on - strand, within MMJJ_RS03440 at 645.661 kb on + strand, within MMJJ_RS03440 at 645.730 kb on - strand, within MMJJ_RS03440 at 645.730 kb on - strand, within MMJJ_RS03440 at 645.730 kb on - strand, within MMJJ_RS03440 at 645.731 kb on + strand, within MMJJ_RS03440 at 645.731 kb on + strand, within MMJJ_RS03440 at 645.731 kb on + strand, within MMJJ_RS03440 at 645.732 kb on - strand, within MMJJ_RS03440 at 645.733 kb on + strand, within MMJJ_RS03440 at 645.733 kb on + strand, within MMJJ_RS03440 at 645.772 kb on + strand, within MMJJ_RS03440 at 645.791 kb on + strand, within MMJJ_RS03440 at 645.834 kb on + strand, within MMJJ_RS03440 at 645.834 kb on + strand, within MMJJ_RS03440 at 645.834 kb on + strand, within MMJJ_RS03440 at 645.837 kb on - strand, within MMJJ_RS03440 at 645.837 kb on - strand, within MMJJ_RS03440 at 645.838 kb on + strand, within MMJJ_RS03440 at 645.838 kb on + strand, within MMJJ_RS03440 at 645.838 kb on + strand, within MMJJ_RS03440 at 645.890 kb on - strand at 645.900 kb on - strand at 645.906 kb on + strand at 645.906 kb on + strand at 645.963 kb on - strand at 645.967 kb on - strand at 645.991 kb on - strand at 646.017 kb on + strand at 646.058 kb on + strand at 646.087 kb on + strand at 646.091 kb on - strand at 646.091 kb on - strand at 646.092 kb on + strand at 646.092 kb on + strand at 646.093 kb on - strand at 646.118 kb on + strand at 646.142 kb on - strand at 646.160 kb on - strand at 646.182 kb on - strand at 646.182 kb on - strand at 646.236 kb on + strand at 646.238 kb on - strand at 646.254 kb on + strand at 646.255 kb on - strand at 646.255 kb on - strand at 646.255 kb on - strand at 646.255 kb on - strand at 646.255 kb on - strand at 646.256 kb on + strand at 646.256 kb on + strand at 646.256 kb on + strand at 646.256 kb on + strand at 646.274 kb on + strand at 646.275 kb on - strand at 646.275 kb on - strand at 646.275 kb on - strand at 646.275 kb on - strand at 646.275 kb on - strand at 646.275 kb on - strand at 646.276 kb on + strand at 646.277 kb on - strand at 646.277 kb on - strand at 646.277 kb on - strand at 646.277 kb on - strand at 646.277 kb on - strand at 646.277 kb on - strand at 646.277 kb on - strand at 646.277 kb on - strand at 646.278 kb on + strand at 646.278 kb on + strand at 646.278 kb on + strand at 646.279 kb on - strand at 646.279 kb on - strand at 646.280 kb on + strand at 646.283 kb on + strand at 646.351 kb on - strand at 646.352 kb on + strand at 646.378 kb on + strand at 646.390 kb on + strand at 646.393 kb on - strand at 646.397 kb on - strand at 646.397 kb on - strand at 646.406 kb on + strand at 646.462 kb on - strand at 646.537 kb on - strand at 646.550 kb on - strand at 646.550 kb on - strand at 646.551 kb on + strand at 646.551 kb on + strand at 646.562 kb on - strand at 646.563 kb on + strand at 646.564 kb on - strand at 646.564 kb on - strand at 646.566 kb on - strand at 646.566 kb on - strand at 646.567 kb on + strand at 646.567 kb on + strand at 646.567 kb on + strand at 646.567 kb on + strand at 646.569 kb on + strand at 646.569 kb on + strand at 646.616 kb on - strand at 646.616 kb on - strand at 646.616 kb on - strand at 646.617 kb on + strand at 646.618 kb on - strand at 646.618 kb on - strand at 646.618 kb on - strand at 646.618 kb on - strand at 646.618 kb on - strand at 646.618 kb on - strand at 646.619 kb on + strand at 646.619 kb on + strand at 646.619 kb on + strand at 646.619 kb on + strand at 646.619 kb on + strand at 646.619 kb on + strand at 646.619 kb on + strand at 646.654 kb on - strand at 646.666 kb on - strand at 646.667 kb on + strand at 646.667 kb on + strand at 646.670 kb on + strand at 646.758 kb on - strand, within MMJJ_RS03445 at 646.759 kb on + strand, within MMJJ_RS03445 at 646.797 kb on - strand, within MMJJ_RS03445 at 646.798 kb on + strand, within MMJJ_RS03445 at 646.798 kb on + strand, within MMJJ_RS03445 at 646.814 kb on + strand, within MMJJ_RS03445 at 646.814 kb on + strand, within MMJJ_RS03445 at 646.892 kb on + strand, within MMJJ_RS03445 at 646.901 kb on + strand, within MMJJ_RS03445 at 646.917 kb on + strand, within MMJJ_RS03445 at 646.934 kb on + strand, within MMJJ_RS03445 at 647.032 kb on + strand, within MMJJ_RS03445 at 647.091 kb on + strand, within MMJJ_RS03445 at 647.092 kb on - strand, within MMJJ_RS03445 at 647.140 kb on - strand at 647.140 kb on - strand at 647.148 kb on - strand at 647.149 kb on + strand at 647.160 kb on - strand at 647.165 kb on - strand at 647.174 kb on - strand at 647.214 kb on - strand at 647.232 kb on - strand at 647.232 kb on - strand at 647.233 kb on + strand at 647.233 kb on + strand at 647.233 kb on + strand at 647.247 kb on - strand at 647.247 kb on - strand at 647.247 kb on - strand at 647.248 kb on + strand at 647.248 kb on + strand at 647.248 kb on + strand at 647.248 kb on + strand at 647.250 kb on + strand at 647.255 kb on - strand at 647.255 kb on - strand at 647.256 kb on + strand at 647.281 kb on + strand, within MMJJ_RS03450 at 647.283 kb on + strand, within MMJJ_RS03450 at 647.296 kb on - strand, within MMJJ_RS03450 at 647.316 kb on - strand, within MMJJ_RS03450 at 647.321 kb on - strand, within MMJJ_RS03450 at 647.322 kb on + strand, within MMJJ_RS03450 at 647.322 kb on + strand, within MMJJ_RS03450 at 647.360 kb on + strand, within MMJJ_RS03450 at 647.422 kb on + strand, within MMJJ_RS03450 at 647.422 kb on + strand, within MMJJ_RS03450 at 647.446 kb on - strand, within MMJJ_RS03450 at 647.489 kb on - strand, within MMJJ_RS03450 at 647.514 kb on - strand, within MMJJ_RS03450 at 647.537 kb on - strand, within MMJJ_RS03450 at 647.537 kb on - strand, within MMJJ_RS03450 at 647.543 kb on - strand, within MMJJ_RS03450 at 647.543 kb on - strand, within MMJJ_RS03450 at 647.563 kb on - strand, within MMJJ_RS03450 at 647.583 kb on - strand at 647.629 kb on - strand at 647.690 kb on - strand at 647.690 kb on - strand at 647.700 kb on + strand at 647.707 kb on - strand at 647.708 kb on + strand at 647.712 kb on - strand at 647.731 kb on + strand at 647.731 kb on + strand at 647.776 kb on + strand at 647.778 kb on - strand at 647.779 kb on + strand at 647.785 kb on + strand at 647.785 kb on + strand at 647.789 kb on - strand at 647.789 kb on - strand at 647.790 kb on + strand at 647.802 kb on - strand, within MMJJ_RS03455 at 647.803 kb on + strand, within MMJJ_RS03455 at 647.809 kb on - strand, within MMJJ_RS03455 at 647.810 kb on + strand, within MMJJ_RS03455 at 647.814 kb on - strand, within MMJJ_RS03455 at 647.842 kb on - strand, within MMJJ_RS03455 at 647.843 kb on + strand, within MMJJ_RS03455 at 647.863 kb on + strand, within MMJJ_RS03455 at 647.877 kb on - strand, within MMJJ_RS03455 at 647.877 kb on - strand, within MMJJ_RS03455 at 647.878 kb on + strand, within MMJJ_RS03455 at 647.878 kb on + strand, within MMJJ_RS03455 at 647.901 kb on - strand, within MMJJ_RS03455 at 647.902 kb on + strand, within MMJJ_RS03455 at 647.902 kb on + strand, within MMJJ_RS03455 at 647.929 kb on + strand, within MMJJ_RS03455 at 647.943 kb on - strand, within MMJJ_RS03455 at 647.943 kb on - strand, within MMJJ_RS03455 at 647.943 kb on - strand, within MMJJ_RS03455 at 647.943 kb on - strand, within MMJJ_RS03455 at 647.944 kb on + strand, within MMJJ_RS03455 at 647.944 kb on + strand, within MMJJ_RS03455 at 647.959 kb on - strand, within MMJJ_RS03455 at 647.960 kb on + strand, within MMJJ_RS03455 at 647.965 kb on - strand, within MMJJ_RS03455 at 647.965 kb on - strand, within MMJJ_RS03455 at 647.976 kb on - strand, within MMJJ_RS03455 at 647.976 kb on - strand, within MMJJ_RS03455 at 648.039 kb on + strand, within MMJJ_RS03455 at 648.082 kb on + strand, within MMJJ_RS03455 at 648.089 kb on - strand, within MMJJ_RS03455 at 648.089 kb on - strand, within MMJJ_RS03455 at 648.089 kb on - strand, within MMJJ_RS03455 at 648.089 kb on - strand, within MMJJ_RS03455 at 648.089 kb on - strand, within MMJJ_RS03455 at 648.090 kb on + strand, within MMJJ_RS03455 at 648.090 kb on + strand, within MMJJ_RS03455 at 648.090 kb on + strand, within MMJJ_RS03455 at 648.093 kb on - strand, within MMJJ_RS03455 at 648.093 kb on - strand, within MMJJ_RS03455 at 648.093 kb on - strand, within MMJJ_RS03455 at 648.094 kb on + strand, within MMJJ_RS03455 at 648.094 kb on + strand, within MMJJ_RS03455 at 648.094 kb on + strand, within MMJJ_RS03455 at 648.094 kb on + strand, within MMJJ_RS03455 at 648.132 kb on - strand, within MMJJ_RS03455 at 648.135 kb on + strand, within MMJJ_RS03455
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM D-glucosamine remove 645,627 - -2.2 645,645 + MMJJ_RS03440 0.14 +0.2 645,646 + MMJJ_RS03440 0.15 +0.6 645,646 + MMJJ_RS03440 0.15 +0.4 645,648 - MMJJ_RS03440 0.15 +0.1 645,661 + MMJJ_RS03440 0.20 +0.8 645,730 - MMJJ_RS03440 0.46 +0.2 645,730 - MMJJ_RS03440 0.46 +0.5 645,730 - MMJJ_RS03440 0.46 +1.1 645,731 + MMJJ_RS03440 0.46 +0.7 645,731 + MMJJ_RS03440 0.46 -0.2 645,731 + MMJJ_RS03440 0.46 +0.6 645,732 - MMJJ_RS03440 0.47 -0.1 645,733 + MMJJ_RS03440 0.47 -2.3 645,733 + MMJJ_RS03440 0.47 +1.3 645,772 + MMJJ_RS03440 0.62 +0.3 645,791 + MMJJ_RS03440 0.69 -0.8 645,834 + MMJJ_RS03440 0.85 -0.9 645,834 + MMJJ_RS03440 0.85 +1.2 645,834 + MMJJ_RS03440 0.85 +0.5 645,837 - MMJJ_RS03440 0.86 +0.7 645,837 - MMJJ_RS03440 0.86 +0.8 645,838 + MMJJ_RS03440 0.87 +0.4 645,838 + MMJJ_RS03440 0.87 +0.0 645,838 + MMJJ_RS03440 0.87 -0.4 645,890 - +0.7 645,900 - +1.4 645,906 + +0.1 645,906 + +0.0 645,963 - -2.1 645,967 - +1.9 645,991 - -0.4 646,017 + +1.0 646,058 + +0.6 646,087 + +0.5 646,091 - -0.2 646,091 - +0.0 646,092 + -0.7 646,092 + -0.6 646,093 - +1.6 646,118 + +0.3 646,142 - +0.9 646,160 - -1.4 646,182 - -0.2 646,182 - +0.8 646,236 + +0.8 646,238 - +0.4 646,254 + -0.4 646,255 - -0.4 646,255 - -0.5 646,255 - -0.6 646,255 - -0.6 646,255 - -0.4 646,256 + -0.1 646,256 + +0.4 646,256 + +0.7 646,256 + -0.0 646,274 + -0.2 646,275 - -2.0 646,275 - -0.9 646,275 - -0.7 646,275 - +0.6 646,275 - +0.5 646,275 - +0.1 646,276 + +0.7 646,277 - -0.5 646,277 - -1.7 646,277 - +0.4 646,277 - -1.4 646,277 - -0.9 646,277 - +0.5 646,277 - +0.9 646,277 - +2.0 646,278 + +0.7 646,278 + +0.7 646,278 + +0.4 646,279 - -2.0 646,279 - -2.4 646,280 + +0.6 646,283 + -1.2 646,351 - +0.2 646,352 + -1.9 646,378 + -0.1 646,390 + -1.0 646,393 - -2.1 646,397 - +1.1 646,397 - -0.0 646,406 + +0.9 646,462 - -0.2 646,537 - -0.6 646,550 - +0.8 646,550 - -2.1 646,551 + +1.1 646,551 + -0.3 646,562 - -0.5 646,563 + -0.2 646,564 - -0.8 646,564 - -1.0 646,566 - -0.9 646,566 - -0.2 646,567 + -0.5 646,567 + -0.4 646,567 + +0.3 646,567 + -0.1 646,569 + -2.7 646,569 + -1.2 646,616 - +0.9 646,616 - -1.9 646,616 - -1.3 646,617 + +1.0 646,618 - +2.5 646,618 - -1.9 646,618 - +0.3 646,618 - +0.4 646,618 - +0.7 646,618 - -1.7 646,619 + +0.3 646,619 + +0.6 646,619 + -0.4 646,619 + +1.8 646,619 + -0.6 646,619 + -0.1 646,619 + -3.0 646,654 - +0.4 646,666 - +1.3 646,667 + -0.1 646,667 + -0.9 646,670 + +0.2 646,758 - MMJJ_RS03445 0.25 -0.2 646,759 + MMJJ_RS03445 0.26 +0.1 646,797 - MMJJ_RS03445 0.32 +1.2 646,798 + MMJJ_RS03445 0.33 -0.3 646,798 + MMJJ_RS03445 0.33 +0.4 646,814 + MMJJ_RS03445 0.36 -1.9 646,814 + MMJJ_RS03445 0.36 +4.2 646,892 + MMJJ_RS03445 0.50 -1.6 646,901 + MMJJ_RS03445 0.51 +0.5 646,917 + MMJJ_RS03445 0.54 +2.4 646,934 + MMJJ_RS03445 0.57 +0.9 647,032 + MMJJ_RS03445 0.75 +0.2 647,091 + MMJJ_RS03445 0.86 +0.5 647,092 - MMJJ_RS03445 0.86 +1.2 647,140 - -2.4 647,140 - +1.0 647,148 - +1.2 647,149 + -1.3 647,160 - -0.1 647,165 - +0.6 647,174 - -0.4 647,214 - +0.4 647,232 - +0.4 647,232 - -1.9 647,233 + +0.1 647,233 + +0.5 647,233 + -0.1 647,247 - -0.0 647,247 - -1.2 647,247 - -0.1 647,248 + +1.0 647,248 + +0.2 647,248 + +0.2 647,248 + +0.3 647,250 + +1.1 647,255 - -1.0 647,255 - -0.2 647,256 + -1.7 647,281 + MMJJ_RS03450 0.16 -0.9 647,283 + MMJJ_RS03450 0.16 +2.1 647,296 - MMJJ_RS03450 0.19 -0.0 647,316 - MMJJ_RS03450 0.24 -2.0 647,321 - MMJJ_RS03450 0.26 +0.8 647,322 + MMJJ_RS03450 0.26 +0.7 647,322 + MMJJ_RS03450 0.26 -0.0 647,360 + MMJJ_RS03450 0.35 -1.9 647,422 + MMJJ_RS03450 0.51 -0.6 647,422 + MMJJ_RS03450 0.51 +1.1 647,446 - MMJJ_RS03450 0.57 -0.0 647,489 - MMJJ_RS03450 0.67 +1.4 647,514 - MMJJ_RS03450 0.74 -1.5 647,537 - MMJJ_RS03450 0.79 +1.1 647,537 - MMJJ_RS03450 0.79 +0.4 647,543 - MMJJ_RS03450 0.81 -0.4 647,543 - MMJJ_RS03450 0.81 +0.5 647,563 - MMJJ_RS03450 0.86 -0.1 647,583 - +1.0 647,629 - +2.1 647,690 - +0.7 647,690 - +0.9 647,700 + +0.2 647,707 - -1.7 647,708 + -1.4 647,712 - +1.0 647,731 + +0.8 647,731 + -0.1 647,776 + +0.0 647,778 - +1.1 647,779 + -0.3 647,785 + -1.1 647,785 + +0.1 647,789 - +0.9 647,789 - -0.2 647,790 + -0.0 647,802 - MMJJ_RS03455 0.10 +0.8 647,803 + MMJJ_RS03455 0.10 +0.4 647,809 - MMJJ_RS03455 0.11 -2.0 647,810 + MMJJ_RS03455 0.11 +0.4 647,814 - MMJJ_RS03455 0.11 +1.2 647,842 - MMJJ_RS03455 0.14 -0.3 647,843 + MMJJ_RS03455 0.14 +0.3 647,863 + MMJJ_RS03455 0.16 -0.2 647,877 - MMJJ_RS03455 0.17 +0.4 647,877 - MMJJ_RS03455 0.17 +0.3 647,878 + MMJJ_RS03455 0.18 +0.4 647,878 + MMJJ_RS03455 0.18 -0.9 647,901 - MMJJ_RS03455 0.20 -0.5 647,902 + MMJJ_RS03455 0.20 -1.2 647,902 + MMJJ_RS03455 0.20 -0.8 647,929 + MMJJ_RS03455 0.23 -1.0 647,943 - MMJJ_RS03455 0.24 -0.3 647,943 - MMJJ_RS03455 0.24 -0.9 647,943 - MMJJ_RS03455 0.24 +0.3 647,943 - MMJJ_RS03455 0.24 -0.6 647,944 + MMJJ_RS03455 0.24 -0.1 647,944 + MMJJ_RS03455 0.24 -0.0 647,959 - MMJJ_RS03455 0.26 -0.5 647,960 + MMJJ_RS03455 0.26 +1.0 647,965 - MMJJ_RS03455 0.26 +0.1 647,965 - MMJJ_RS03455 0.26 +0.7 647,976 - MMJJ_RS03455 0.27 +0.0 647,976 - MMJJ_RS03455 0.27 -0.1 648,039 + MMJJ_RS03455 0.33 +0.6 648,082 + MMJJ_RS03455 0.38 +0.1 648,089 - MMJJ_RS03455 0.38 +0.5 648,089 - MMJJ_RS03455 0.38 +0.2 648,089 - MMJJ_RS03455 0.38 +0.0 648,089 - MMJJ_RS03455 0.38 -0.6 648,089 - MMJJ_RS03455 0.38 -1.2 648,090 + MMJJ_RS03455 0.38 -0.3 648,090 + MMJJ_RS03455 0.38 -0.6 648,090 + MMJJ_RS03455 0.38 +2.0 648,093 - MMJJ_RS03455 0.39 +0.5 648,093 - MMJJ_RS03455 0.39 -0.2 648,093 - MMJJ_RS03455 0.39 +0.3 648,094 + MMJJ_RS03455 0.39 +0.2 648,094 + MMJJ_RS03455 0.39 -1.2 648,094 + MMJJ_RS03455 0.39 -1.0 648,094 + MMJJ_RS03455 0.39 +0.8 648,132 - MMJJ_RS03455 0.42 -0.8 648,135 + MMJJ_RS03455 0.43 +0.1
Or see this region's nucleotide sequence