Experiment: H2 N-free with 10mM D-glucosamine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03230 and MMJJ_RS03235 are separated by 32 nucleotides MMJJ_RS03235 and MMJJ_RS03240 are separated by 289 nucleotides MMJJ_RS03240 and MMJJ_RS03245 are separated by 236 nucleotides
MMJJ_RS03230: MMJJ_RS03230 - NAD(P)/FAD-dependent oxidoreductase, at 611,538 to 612,710
_RS03230
MMJJ_RS03235: MMJJ_RS03235 - hypothetical protein, at 612,743 to 613,264
_RS03235
MMJJ_RS03240: MMJJ_RS03240 - histone family protein, at 613,554 to 613,757
_RS03240
MMJJ_RS03245: MMJJ_RS03245 - UMP kinase, at 613,994 to 614,671
_RS03245
Position (kb) 612
613
614 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 612.012 kb on + strand, within MMJJ_RS03230 at 612.153 kb on - strand, within MMJJ_RS03230 at 612.180 kb on + strand, within MMJJ_RS03230 at 612.191 kb on + strand, within MMJJ_RS03230 at 612.235 kb on - strand, within MMJJ_RS03230 at 612.235 kb on - strand, within MMJJ_RS03230 at 612.236 kb on + strand, within MMJJ_RS03230 at 612.385 kb on - strand, within MMJJ_RS03230 at 612.410 kb on + strand, within MMJJ_RS03230 at 612.477 kb on - strand, within MMJJ_RS03230 at 612.634 kb on - strand at 612.635 kb on + strand at 612.635 kb on + strand at 612.635 kb on + strand at 612.657 kb on + strand at 612.661 kb on - strand at 612.704 kb on + strand at 612.721 kb on - strand at 612.721 kb on - strand at 612.722 kb on + strand at 612.722 kb on + strand at 612.722 kb on + strand at 612.722 kb on + strand at 612.737 kb on - strand at 612.780 kb on - strand at 612.780 kb on - strand at 612.780 kb on - strand at 612.780 kb on - strand at 612.781 kb on + strand at 612.783 kb on - strand at 612.783 kb on - strand at 612.784 kb on + strand at 612.784 kb on + strand at 612.784 kb on + strand at 612.792 kb on + strand at 612.794 kb on - strand at 612.794 kb on - strand at 612.794 kb on - strand at 612.794 kb on - strand at 612.812 kb on - strand, within MMJJ_RS03235 at 612.812 kb on - strand, within MMJJ_RS03235 at 612.813 kb on + strand, within MMJJ_RS03235 at 612.814 kb on - strand, within MMJJ_RS03235 at 612.815 kb on + strand, within MMJJ_RS03235 at 612.819 kb on + strand, within MMJJ_RS03235 at 612.819 kb on + strand, within MMJJ_RS03235 at 612.819 kb on + strand, within MMJJ_RS03235 at 612.836 kb on - strand, within MMJJ_RS03235 at 612.864 kb on + strand, within MMJJ_RS03235 at 613.056 kb on - strand, within MMJJ_RS03235 at 613.348 kb on - strand at 613.400 kb on + strand at 613.403 kb on + strand at 613.405 kb on - strand at 613.406 kb on + strand at 613.408 kb on - strand at 613.410 kb on - strand at 613.410 kb on - strand at 613.410 kb on - strand at 613.411 kb on + strand at 613.411 kb on + strand at 613.415 kb on + strand at 613.415 kb on + strand at 613.420 kb on + strand at 613.422 kb on - strand at 613.425 kb on + strand at 613.425 kb on + strand at 613.429 kb on - strand at 613.430 kb on + strand at 613.430 kb on + strand at 613.449 kb on + strand at 613.449 kb on + strand at 613.469 kb on + strand at 613.507 kb on + strand at 613.507 kb on + strand at 613.507 kb on + strand at 613.553 kb on - strand at 613.565 kb on + strand at 613.582 kb on - strand, within MMJJ_RS03240 at 613.588 kb on - strand, within MMJJ_RS03240 at 613.616 kb on + strand, within MMJJ_RS03240 at 613.756 kb on + strand at 613.766 kb on - strand at 613.767 kb on + strand at 613.845 kb on - strand at 613.846 kb on + strand at 613.858 kb on - strand at 613.859 kb on + strand at 613.864 kb on + strand at 613.864 kb on + strand at 613.917 kb on + strand at 613.930 kb on + strand at 614.002 kb on + strand at 614.150 kb on - strand, within MMJJ_RS03245
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM D-glucosamine remove 612,012 + MMJJ_RS03230 0.40 +0.5 612,153 - MMJJ_RS03230 0.52 -1.4 612,180 + MMJJ_RS03230 0.55 -1.9 612,191 + MMJJ_RS03230 0.56 -1.5 612,235 - MMJJ_RS03230 0.59 -0.0 612,235 - MMJJ_RS03230 0.59 -1.3 612,236 + MMJJ_RS03230 0.60 -1.9 612,385 - MMJJ_RS03230 0.72 -1.8 612,410 + MMJJ_RS03230 0.74 -1.5 612,477 - MMJJ_RS03230 0.80 -2.0 612,634 - -2.4 612,635 + -1.2 612,635 + -2.1 612,635 + +0.5 612,657 + -1.3 612,661 - +0.4 612,704 + +0.3 612,721 - +0.4 612,721 - -1.6 612,722 + -0.8 612,722 + +0.3 612,722 + -0.4 612,722 + -1.1 612,737 - +3.8 612,780 - -1.1 612,780 - +0.1 612,780 - +0.9 612,780 - -0.5 612,781 + +1.3 612,783 - +0.8 612,783 - +0.7 612,784 + +0.8 612,784 + -0.6 612,784 + +0.6 612,792 + -0.5 612,794 - -0.7 612,794 - -1.0 612,794 - -0.2 612,794 - +0.3 612,812 - MMJJ_RS03235 0.13 +0.5 612,812 - MMJJ_RS03235 0.13 -1.8 612,813 + MMJJ_RS03235 0.13 +1.1 612,814 - MMJJ_RS03235 0.14 +0.1 612,815 + MMJJ_RS03235 0.14 +0.2 612,819 + MMJJ_RS03235 0.15 +1.3 612,819 + MMJJ_RS03235 0.15 +0.9 612,819 + MMJJ_RS03235 0.15 -0.5 612,836 - MMJJ_RS03235 0.18 +1.6 612,864 + MMJJ_RS03235 0.23 -0.6 613,056 - MMJJ_RS03235 0.60 -1.7 613,348 - +0.1 613,400 + -0.6 613,403 + -2.3 613,405 - -1.5 613,406 + +1.2 613,408 - +0.4 613,410 - -0.2 613,410 - +4.7 613,410 - -0.2 613,411 + +2.1 613,411 + +0.9 613,415 + -0.9 613,415 + +1.1 613,420 + +1.1 613,422 - +0.3 613,425 + -2.1 613,425 + -1.1 613,429 - -1.1 613,430 + -1.4 613,430 + +0.5 613,449 + -0.3 613,449 + -0.2 613,469 + +0.1 613,507 + -1.2 613,507 + -1.1 613,507 + -1.9 613,553 - -1.2 613,565 + -1.4 613,582 - MMJJ_RS03240 0.14 -1.5 613,588 - MMJJ_RS03240 0.17 -0.1 613,616 + MMJJ_RS03240 0.30 -2.9 613,756 + -0.3 613,766 - -2.7 613,767 + +0.2 613,845 - +2.3 613,846 + -0.7 613,858 - +3.9 613,859 + -0.2 613,864 + +0.6 613,864 + +1.7 613,917 + +0.5 613,930 + +0.1 614,002 + -0.7 614,150 - MMJJ_RS03245 0.23 -2.1
Or see this region's nucleotide sequence