Experiment: H2 N-free with 10mM D-glucosamine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS00140 and MMJJ_RS00145 are separated by 125 nucleotides MMJJ_RS00145 and MMJJ_RS00150 overlap by 4 nucleotides MMJJ_RS00150 and MMJJ_RS00155 are separated by 35 nucleotides
MMJJ_RS00140: MMJJ_RS00140 - hypothetical protein, at 26,335 to 26,592
_RS00140
MMJJ_RS00145: MMJJ_RS00145 - aquaporin family protein, at 26,718 to 27,437
_RS00145
MMJJ_RS00150: MMJJ_RS00150 - DUF2180 family protein, at 27,434 to 27,649
_RS00150
MMJJ_RS00155: MMJJ_RS00155 - DUF2193 domain-containing protein, at 27,685 to 29,184
_RS00155
Position (kb) 27
28 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 26.574 kb on + strand at 26.583 kb on - strand at 26.604 kb on - strand at 26.609 kb on + strand at 26.609 kb on + strand at 26.609 kb on + strand at 26.611 kb on - strand at 26.614 kb on - strand at 26.614 kb on - strand at 26.615 kb on + strand at 26.619 kb on + strand at 26.730 kb on - strand at 26.730 kb on - strand at 26.730 kb on - strand at 26.740 kb on - strand at 26.740 kb on - strand at 26.741 kb on + strand at 26.744 kb on - strand at 26.744 kb on - strand at 26.744 kb on - strand at 26.745 kb on + strand at 26.757 kb on - strand at 26.757 kb on - strand at 26.757 kb on - strand at 26.757 kb on - strand at 26.758 kb on + strand at 26.783 kb on - strand at 26.783 kb on - strand at 26.783 kb on - strand at 26.783 kb on - strand at 26.784 kb on + strand at 26.784 kb on + strand at 26.784 kb on + strand at 26.785 kb on - strand at 26.785 kb on - strand at 26.785 kb on - strand at 26.785 kb on - strand at 26.785 kb on - strand at 26.785 kb on - strand at 26.786 kb on + strand at 26.786 kb on + strand at 26.786 kb on + strand at 26.786 kb on + strand at 26.786 kb on + strand at 26.786 kb on + strand at 26.786 kb on + strand at 26.788 kb on + strand at 26.797 kb on - strand, within MMJJ_RS00145 at 26.797 kb on - strand, within MMJJ_RS00145 at 26.797 kb on - strand, within MMJJ_RS00145 at 26.801 kb on + strand, within MMJJ_RS00145 at 26.801 kb on + strand, within MMJJ_RS00145 at 26.807 kb on - strand, within MMJJ_RS00145 at 26.822 kb on - strand, within MMJJ_RS00145 at 26.823 kb on + strand, within MMJJ_RS00145 at 26.823 kb on + strand, within MMJJ_RS00145 at 26.825 kb on - strand, within MMJJ_RS00145 at 26.825 kb on - strand, within MMJJ_RS00145 at 26.825 kb on - strand, within MMJJ_RS00145 at 26.826 kb on + strand, within MMJJ_RS00145 at 26.826 kb on + strand, within MMJJ_RS00145 at 26.826 kb on + strand, within MMJJ_RS00145 at 26.826 kb on + strand, within MMJJ_RS00145 at 26.826 kb on + strand, within MMJJ_RS00145 at 26.829 kb on - strand, within MMJJ_RS00145 at 26.830 kb on + strand, within MMJJ_RS00145 at 26.863 kb on + strand, within MMJJ_RS00145 at 26.880 kb on + strand, within MMJJ_RS00145 at 26.973 kb on - strand, within MMJJ_RS00145 at 26.975 kb on - strand, within MMJJ_RS00145 at 27.003 kb on + strand, within MMJJ_RS00145 at 27.075 kb on - strand, within MMJJ_RS00145 at 27.108 kb on - strand, within MMJJ_RS00145 at 27.133 kb on + strand, within MMJJ_RS00145 at 27.146 kb on - strand, within MMJJ_RS00145 at 27.146 kb on - strand, within MMJJ_RS00145 at 27.147 kb on + strand, within MMJJ_RS00145 at 27.147 kb on + strand, within MMJJ_RS00145 at 27.148 kb on - strand, within MMJJ_RS00145 at 27.149 kb on + strand, within MMJJ_RS00145 at 27.150 kb on - strand, within MMJJ_RS00145 at 27.150 kb on - strand, within MMJJ_RS00145 at 27.150 kb on - strand, within MMJJ_RS00145 at 27.150 kb on - strand, within MMJJ_RS00145 at 27.150 kb on - strand, within MMJJ_RS00145 at 27.151 kb on + strand, within MMJJ_RS00145 at 27.151 kb on + strand, within MMJJ_RS00145 at 27.151 kb on + strand, within MMJJ_RS00145 at 27.151 kb on + strand, within MMJJ_RS00145 at 27.159 kb on + strand, within MMJJ_RS00145 at 27.200 kb on - strand, within MMJJ_RS00145 at 27.201 kb on + strand, within MMJJ_RS00145 at 27.213 kb on + strand, within MMJJ_RS00145 at 27.230 kb on - strand, within MMJJ_RS00145 at 27.230 kb on - strand, within MMJJ_RS00145 at 27.242 kb on - strand, within MMJJ_RS00145 at 27.242 kb on - strand, within MMJJ_RS00145 at 27.242 kb on - strand, within MMJJ_RS00145 at 27.249 kb on - strand, within MMJJ_RS00145 at 27.250 kb on + strand, within MMJJ_RS00145 at 27.393 kb on + strand at 27.451 kb on - strand at 27.452 kb on + strand at 27.463 kb on + strand, within MMJJ_RS00150 at 27.463 kb on + strand, within MMJJ_RS00150 at 27.554 kb on - strand, within MMJJ_RS00150 at 27.554 kb on - strand, within MMJJ_RS00150 at 27.555 kb on + strand, within MMJJ_RS00150 at 27.557 kb on - strand, within MMJJ_RS00150 at 27.557 kb on - strand, within MMJJ_RS00150 at 27.557 kb on - strand, within MMJJ_RS00150 at 27.557 kb on - strand, within MMJJ_RS00150 at 27.558 kb on + strand, within MMJJ_RS00150 at 27.558 kb on + strand, within MMJJ_RS00150 at 27.558 kb on + strand, within MMJJ_RS00150 at 27.568 kb on - strand, within MMJJ_RS00150 at 27.568 kb on - strand, within MMJJ_RS00150 at 27.590 kb on - strand, within MMJJ_RS00150 at 27.591 kb on + strand, within MMJJ_RS00150 at 27.602 kb on - strand, within MMJJ_RS00150 at 27.602 kb on - strand, within MMJJ_RS00150 at 27.603 kb on + strand, within MMJJ_RS00150 at 27.639 kb on - strand at 27.639 kb on - strand at 27.640 kb on + strand at 27.653 kb on + strand at 27.675 kb on - strand at 27.676 kb on + strand at 27.680 kb on + strand at 27.686 kb on - strand at 27.686 kb on - strand at 27.688 kb on - strand at 27.689 kb on + strand at 27.710 kb on - strand at 27.720 kb on - strand at 27.905 kb on - strand, within MMJJ_RS00155 at 27.911 kb on - strand, within MMJJ_RS00155 at 27.912 kb on + strand, within MMJJ_RS00155 at 27.931 kb on + strand, within MMJJ_RS00155 at 27.961 kb on - strand, within MMJJ_RS00155 at 27.962 kb on + strand, within MMJJ_RS00155 at 27.977 kb on + strand, within MMJJ_RS00155 at 27.989 kb on + strand, within MMJJ_RS00155 at 28.047 kb on - strand, within MMJJ_RS00155 at 28.047 kb on - strand, within MMJJ_RS00155 at 28.049 kb on - strand, within MMJJ_RS00155 at 28.049 kb on - strand, within MMJJ_RS00155 at 28.049 kb on - strand, within MMJJ_RS00155 at 28.049 kb on - strand, within MMJJ_RS00155 at 28.049 kb on - strand, within MMJJ_RS00155 at 28.050 kb on + strand, within MMJJ_RS00155 at 28.050 kb on + strand, within MMJJ_RS00155 at 28.051 kb on - strand, within MMJJ_RS00155 at 28.051 kb on - strand, within MMJJ_RS00155 at 28.051 kb on - strand, within MMJJ_RS00155 at 28.051 kb on - strand, within MMJJ_RS00155 at 28.051 kb on - strand, within MMJJ_RS00155 at 28.051 kb on - strand, within MMJJ_RS00155 at 28.051 kb on - strand, within MMJJ_RS00155 at 28.051 kb on - strand, within MMJJ_RS00155 at 28.051 kb on - strand, within MMJJ_RS00155 at 28.052 kb on + strand, within MMJJ_RS00155 at 28.052 kb on + strand, within MMJJ_RS00155 at 28.052 kb on + strand, within MMJJ_RS00155 at 28.052 kb on + strand, within MMJJ_RS00155 at 28.052 kb on + strand, within MMJJ_RS00155 at 28.114 kb on - strand, within MMJJ_RS00155 at 28.115 kb on + strand, within MMJJ_RS00155 at 28.118 kb on - strand, within MMJJ_RS00155 at 28.118 kb on - strand, within MMJJ_RS00155 at 28.119 kb on + strand, within MMJJ_RS00155 at 28.119 kb on + strand, within MMJJ_RS00155 at 28.119 kb on + strand, within MMJJ_RS00155 at 28.273 kb on + strand, within MMJJ_RS00155 at 28.273 kb on + strand, within MMJJ_RS00155 at 28.273 kb on + strand, within MMJJ_RS00155 at 28.273 kb on + strand, within MMJJ_RS00155 at 28.313 kb on + strand, within MMJJ_RS00155 at 28.335 kb on - strand, within MMJJ_RS00155 at 28.336 kb on + strand, within MMJJ_RS00155 at 28.379 kb on - strand, within MMJJ_RS00155 at 28.379 kb on - strand, within MMJJ_RS00155 at 28.389 kb on - strand, within MMJJ_RS00155 at 28.389 kb on - strand, within MMJJ_RS00155 at 28.389 kb on - strand, within MMJJ_RS00155 at 28.389 kb on - strand, within MMJJ_RS00155 at 28.390 kb on + strand, within MMJJ_RS00155 at 28.393 kb on - strand, within MMJJ_RS00155 at 28.435 kb on - strand, within MMJJ_RS00155 at 28.472 kb on - strand, within MMJJ_RS00155 at 28.545 kb on + strand, within MMJJ_RS00155 at 28.575 kb on - strand, within MMJJ_RS00155 at 28.637 kb on - strand, within MMJJ_RS00155
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM D-glucosamine remove 26,574 + -2.0 26,583 - -2.6 26,604 - +0.8 26,609 + -1.9 26,609 + -0.8 26,609 + +1.2 26,611 - -0.8 26,614 - +0.2 26,614 - -1.4 26,615 + +0.2 26,619 + -1.0 26,730 - -0.1 26,730 - -1.4 26,730 - +0.3 26,740 - -0.4 26,740 - -0.7 26,741 + -0.8 26,744 - -0.1 26,744 - -1.0 26,744 - +0.3 26,745 + -0.1 26,757 - +1.0 26,757 - -0.1 26,757 - +1.3 26,757 - -0.6 26,758 + +0.1 26,783 - +0.2 26,783 - -1.6 26,783 - -0.6 26,783 - +0.2 26,784 + -0.1 26,784 + +0.2 26,784 + +0.6 26,785 - +0.9 26,785 - -0.1 26,785 - +0.6 26,785 - +3.0 26,785 - +0.4 26,785 - +0.3 26,786 + +0.0 26,786 + -0.8 26,786 + +1.2 26,786 + -1.2 26,786 + +0.1 26,786 + +0.4 26,786 + -2.6 26,788 + -0.5 26,797 - MMJJ_RS00145 0.11 -0.1 26,797 - MMJJ_RS00145 0.11 +0.3 26,797 - MMJJ_RS00145 0.11 +0.6 26,801 + MMJJ_RS00145 0.12 +0.6 26,801 + MMJJ_RS00145 0.12 +0.2 26,807 - MMJJ_RS00145 0.12 -0.7 26,822 - MMJJ_RS00145 0.14 -0.8 26,823 + MMJJ_RS00145 0.15 -1.9 26,823 + MMJJ_RS00145 0.15 +0.3 26,825 - MMJJ_RS00145 0.15 -1.3 26,825 - MMJJ_RS00145 0.15 +0.9 26,825 - MMJJ_RS00145 0.15 +0.2 26,826 + MMJJ_RS00145 0.15 +0.8 26,826 + MMJJ_RS00145 0.15 -0.0 26,826 + MMJJ_RS00145 0.15 +1.0 26,826 + MMJJ_RS00145 0.15 +5.5 26,826 + MMJJ_RS00145 0.15 -0.2 26,829 - MMJJ_RS00145 0.15 -1.5 26,830 + MMJJ_RS00145 0.16 +0.4 26,863 + MMJJ_RS00145 0.20 -0.0 26,880 + MMJJ_RS00145 0.23 -0.8 26,973 - MMJJ_RS00145 0.35 -0.1 26,975 - MMJJ_RS00145 0.36 -0.4 27,003 + MMJJ_RS00145 0.40 +0.9 27,075 - MMJJ_RS00145 0.50 -0.1 27,108 - MMJJ_RS00145 0.54 -0.1 27,133 + MMJJ_RS00145 0.58 +1.2 27,146 - MMJJ_RS00145 0.59 +0.1 27,146 - MMJJ_RS00145 0.59 -1.8 27,147 + MMJJ_RS00145 0.60 -0.6 27,147 + MMJJ_RS00145 0.60 +0.3 27,148 - MMJJ_RS00145 0.60 -1.1 27,149 + MMJJ_RS00145 0.60 +0.1 27,150 - MMJJ_RS00145 0.60 +1.0 27,150 - MMJJ_RS00145 0.60 -0.2 27,150 - MMJJ_RS00145 0.60 +0.3 27,150 - MMJJ_RS00145 0.60 +1.0 27,150 - MMJJ_RS00145 0.60 -0.9 27,151 + MMJJ_RS00145 0.60 -1.7 27,151 + MMJJ_RS00145 0.60 +0.3 27,151 + MMJJ_RS00145 0.60 -1.1 27,151 + MMJJ_RS00145 0.60 -1.5 27,159 + MMJJ_RS00145 0.61 +0.1 27,200 - MMJJ_RS00145 0.67 +0.6 27,201 + MMJJ_RS00145 0.67 -0.1 27,213 + MMJJ_RS00145 0.69 +0.1 27,230 - MMJJ_RS00145 0.71 -0.9 27,230 - MMJJ_RS00145 0.71 -0.3 27,242 - MMJJ_RS00145 0.73 +0.3 27,242 - MMJJ_RS00145 0.73 -0.6 27,242 - MMJJ_RS00145 0.73 -0.4 27,249 - MMJJ_RS00145 0.74 -0.2 27,250 + MMJJ_RS00145 0.74 +2.6 27,393 + -1.5 27,451 - -0.3 27,452 + -3.5 27,463 + MMJJ_RS00150 0.13 -1.4 27,463 + MMJJ_RS00150 0.13 +0.2 27,554 - MMJJ_RS00150 0.56 +0.1 27,554 - MMJJ_RS00150 0.56 +0.2 27,555 + MMJJ_RS00150 0.56 -0.1 27,557 - MMJJ_RS00150 0.57 +1.0 27,557 - MMJJ_RS00150 0.57 -0.4 27,557 - MMJJ_RS00150 0.57 +5.1 27,557 - MMJJ_RS00150 0.57 -0.0 27,558 + MMJJ_RS00150 0.57 +0.8 27,558 + MMJJ_RS00150 0.57 +1.7 27,558 + MMJJ_RS00150 0.57 -0.3 27,568 - MMJJ_RS00150 0.62 -0.2 27,568 - MMJJ_RS00150 0.62 -0.6 27,590 - MMJJ_RS00150 0.72 -0.1 27,591 + MMJJ_RS00150 0.73 +0.7 27,602 - MMJJ_RS00150 0.78 -0.6 27,602 - MMJJ_RS00150 0.78 +0.0 27,603 + MMJJ_RS00150 0.78 -0.2 27,639 - +0.4 27,639 - -0.8 27,640 + -0.2 27,653 + -0.8 27,675 - +0.1 27,676 + +0.6 27,680 + +1.6 27,686 - -0.2 27,686 - +0.1 27,688 - +0.0 27,689 + -0.0 27,710 - -0.7 27,720 - -0.2 27,905 - MMJJ_RS00155 0.15 -0.5 27,911 - MMJJ_RS00155 0.15 -0.6 27,912 + MMJJ_RS00155 0.15 +0.3 27,931 + MMJJ_RS00155 0.16 +0.6 27,961 - MMJJ_RS00155 0.18 +0.7 27,962 + MMJJ_RS00155 0.18 -0.0 27,977 + MMJJ_RS00155 0.19 +0.1 27,989 + MMJJ_RS00155 0.20 +0.1 28,047 - MMJJ_RS00155 0.24 +1.0 28,047 - MMJJ_RS00155 0.24 -1.3 28,049 - MMJJ_RS00155 0.24 +0.7 28,049 - MMJJ_RS00155 0.24 -0.3 28,049 - MMJJ_RS00155 0.24 -0.4 28,049 - MMJJ_RS00155 0.24 +0.2 28,049 - MMJJ_RS00155 0.24 +0.3 28,050 + MMJJ_RS00155 0.24 -0.2 28,050 + MMJJ_RS00155 0.24 +0.6 28,051 - MMJJ_RS00155 0.24 -0.3 28,051 - MMJJ_RS00155 0.24 -1.2 28,051 - MMJJ_RS00155 0.24 +0.3 28,051 - MMJJ_RS00155 0.24 -0.5 28,051 - MMJJ_RS00155 0.24 -0.6 28,051 - MMJJ_RS00155 0.24 -1.1 28,051 - MMJJ_RS00155 0.24 +1.5 28,051 - MMJJ_RS00155 0.24 -0.1 28,051 - MMJJ_RS00155 0.24 -0.3 28,052 + MMJJ_RS00155 0.24 +0.7 28,052 + MMJJ_RS00155 0.24 +0.7 28,052 + MMJJ_RS00155 0.24 -1.8 28,052 + MMJJ_RS00155 0.24 -0.6 28,052 + MMJJ_RS00155 0.24 +0.5 28,114 - MMJJ_RS00155 0.29 -0.4 28,115 + MMJJ_RS00155 0.29 -0.9 28,118 - MMJJ_RS00155 0.29 +0.1 28,118 - MMJJ_RS00155 0.29 -0.1 28,119 + MMJJ_RS00155 0.29 +0.4 28,119 + MMJJ_RS00155 0.29 -1.2 28,119 + MMJJ_RS00155 0.29 -1.2 28,273 + MMJJ_RS00155 0.39 -0.4 28,273 + MMJJ_RS00155 0.39 -0.0 28,273 + MMJJ_RS00155 0.39 -2.4 28,273 + MMJJ_RS00155 0.39 +0.2 28,313 + MMJJ_RS00155 0.42 +0.6 28,335 - MMJJ_RS00155 0.43 -0.1 28,336 + MMJJ_RS00155 0.43 +0.6 28,379 - MMJJ_RS00155 0.46 -0.0 28,379 - MMJJ_RS00155 0.46 -0.0 28,389 - MMJJ_RS00155 0.47 -0.2 28,389 - MMJJ_RS00155 0.47 -0.6 28,389 - MMJJ_RS00155 0.47 +0.6 28,389 - MMJJ_RS00155 0.47 -0.4 28,390 + MMJJ_RS00155 0.47 -0.8 28,393 - MMJJ_RS00155 0.47 -0.3 28,435 - MMJJ_RS00155 0.50 +2.0 28,472 - MMJJ_RS00155 0.52 +0.3 28,545 + MMJJ_RS00155 0.57 -1.9 28,575 - MMJJ_RS00155 0.59 -1.7 28,637 - MMJJ_RS00155 0.63 +0.2
Or see this region's nucleotide sequence