Experiment: Choline Lysine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3754 and Shew_3755 are separated by 65 nucleotides Shew_3755 and Shew_3756 are separated by 150 nucleotides Shew_3756 and Shew_3757 are separated by 27 nucleotides
Shew_3754: Shew_3754 - hypothetical protein (RefSeq), at 4,470,544 to 4,471,221
_3754
Shew_3755: Shew_3755 - thioesterase superfamily protein (RefSeq), at 4,471,287 to 4,471,766
_3755
Shew_3756: Shew_3756 - histidine ammonia-lyase (RefSeq), at 4,471,917 to 4,473,449
_3756
Shew_3757: Shew_3757 - urocanate hydratase (RefSeq), at 4,473,477 to 4,475,147
_3757
Position (kb)
4471
4472
4473
4474 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4470.987 kb on - strand, within Shew_3754 at 4471.050 kb on + strand, within Shew_3754 at 4471.058 kb on - strand, within Shew_3754 at 4471.159 kb on + strand at 4471.167 kb on - strand at 4471.378 kb on - strand, within Shew_3755 at 4471.473 kb on - strand, within Shew_3755 at 4471.830 kb on + strand at 4471.921 kb on + strand at 4471.921 kb on + strand at 4472.050 kb on + strand at 4472.058 kb on - strand at 4472.058 kb on - strand at 4472.058 kb on - strand at 4472.087 kb on - strand, within Shew_3756 at 4472.241 kb on + strand, within Shew_3756 at 4472.284 kb on - strand, within Shew_3756 at 4472.400 kb on + strand, within Shew_3756 at 4472.647 kb on - strand, within Shew_3756 at 4472.744 kb on + strand, within Shew_3756 at 4472.744 kb on + strand, within Shew_3756 at 4472.845 kb on + strand, within Shew_3756 at 4472.853 kb on - strand, within Shew_3756 at 4472.868 kb on - strand, within Shew_3756 at 4472.911 kb on - strand, within Shew_3756 at 4472.968 kb on + strand, within Shew_3756 at 4473.177 kb on + strand, within Shew_3756 at 4473.368 kb on + strand at 4473.368 kb on + strand at 4473.368 kb on + strand at 4473.368 kb on + strand at 4473.376 kb on - strand at 4473.376 kb on - strand at 4473.421 kb on - strand at 4473.602 kb on - strand at 4473.631 kb on - strand at 4473.796 kb on - strand, within Shew_3757 at 4474.136 kb on - strand, within Shew_3757 at 4474.225 kb on + strand, within Shew_3757 at 4474.225 kb on + strand, within Shew_3757 at 4474.225 kb on + strand, within Shew_3757 at 4474.225 kb on + strand, within Shew_3757 at 4474.233 kb on - strand, within Shew_3757 at 4474.233 kb on - strand, within Shew_3757 at 4474.312 kb on + strand, within Shew_3757 at 4474.312 kb on + strand, within Shew_3757
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 10 mM remove 4,470,987 - Shew_3754 0.65 +0.1 4,471,050 + Shew_3754 0.75 -0.0 4,471,058 - Shew_3754 0.76 +0.5 4,471,159 + -0.1 4,471,167 - +0.1 4,471,378 - Shew_3755 0.19 +0.4 4,471,473 - Shew_3755 0.39 -0.1 4,471,830 + -0.2 4,471,921 + -0.9 4,471,921 + -1.1 4,472,050 + -1.1 4,472,058 - -1.2 4,472,058 - -0.9 4,472,058 - -0.5 4,472,087 - Shew_3756 0.11 -0.7 4,472,241 + Shew_3756 0.21 -0.7 4,472,284 - Shew_3756 0.24 -1.3 4,472,400 + Shew_3756 0.32 -0.9 4,472,647 - Shew_3756 0.48 -0.9 4,472,744 + Shew_3756 0.54 -1.2 4,472,744 + Shew_3756 0.54 -1.1 4,472,845 + Shew_3756 0.61 -0.4 4,472,853 - Shew_3756 0.61 -0.8 4,472,868 - Shew_3756 0.62 -1.3 4,472,911 - Shew_3756 0.65 -1.0 4,472,968 + Shew_3756 0.69 -1.0 4,473,177 + Shew_3756 0.82 -0.6 4,473,368 + -2.2 4,473,368 + -1.0 4,473,368 + -1.2 4,473,368 + -3.6 4,473,376 - -0.8 4,473,376 - -0.5 4,473,421 - -2.0 4,473,602 - +1.5 4,473,631 - -0.2 4,473,796 - Shew_3757 0.19 -0.6 4,474,136 - Shew_3757 0.39 -1.7 4,474,225 + Shew_3757 0.45 -0.3 4,474,225 + Shew_3757 0.45 -0.7 4,474,225 + Shew_3757 0.45 -0.4 4,474,225 + Shew_3757 0.45 -1.2 4,474,233 - Shew_3757 0.45 -3.4 4,474,233 - Shew_3757 0.45 -1.7 4,474,312 + Shew_3757 0.50 -2.2 4,474,312 + Shew_3757 0.50 -1.0
Or see this region's nucleotide sequence