Experiment: Choline Lysine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0306 and Shew_R0014 are separated by 218 nucleotides Shew_R0014 and Shew_R0015 are separated by 43 nucleotides Shew_R0015 and Shew_R0016 are separated by 84 nucleotides Shew_R0016 and Shew_R0017 are separated by 52 nucleotides Shew_R0017 and Shew_0307 are separated by 132 nucleotides Shew_0307 and Shew_0308 are separated by 8 nucleotides Shew_0308 and Shew_0309 are separated by 16 nucleotides
Shew_0306: Shew_0306 - diguanylate cyclase/phosphodiesterase with GAF sensor (RefSeq), at 329,116 to 331,764
_0306
Shew_R0014: Shew_R0014 - tRNA-Arg (RefSeq), at 331,983 to 332,059
_R0014
Shew_R0015: Shew_R0015 - tRNA-His (RefSeq), at 332,103 to 332,178
_R0015
Shew_R0016: Shew_R0016 - tRNA-Pro (RefSeq), at 332,263 to 332,339
_R0016
Shew_R0017: Shew_R0017 - tRNA-Pro (RefSeq), at 332,392 to 332,468
_R0017
Shew_0307: Shew_0307 - diguanylate cyclase/phosphodiesterase (RefSeq), at 332,601 to 334,523
_0307
Shew_0308: Shew_0308 - hypothetical protein (RefSeq), at 334,532 to 335,239
_0308
Shew_0309: Shew_0309 - OmpA/MotB domain-containing protein (RefSeq), at 335,256 to 335,861
_0309
Position (kb) 332
333
334
335 Strain fitness (log2 ratio) -2
-1
0
1 at 331.986 kb on + strand at 332.850 kb on - strand, within Shew_0307 at 333.130 kb on - strand, within Shew_0307 at 333.311 kb on + strand, within Shew_0307 at 333.319 kb on + strand, within Shew_0307 at 333.409 kb on - strand, within Shew_0307 at 333.447 kb on + strand, within Shew_0307 at 333.753 kb on + strand, within Shew_0307 at 333.838 kb on + strand, within Shew_0307 at 334.028 kb on + strand, within Shew_0307 at 334.074 kb on + strand, within Shew_0307 at 334.405 kb on + strand at 334.409 kb on + strand at 334.498 kb on + strand at 334.669 kb on + strand, within Shew_0308 at 334.783 kb on - strand, within Shew_0308 at 334.911 kb on - strand, within Shew_0308 at 334.940 kb on - strand, within Shew_0308 at 334.940 kb on - strand, within Shew_0308 at 334.974 kb on + strand, within Shew_0308 at 335.049 kb on + strand, within Shew_0308 at 335.058 kb on - strand, within Shew_0308 at 335.173 kb on - strand at 335.173 kb on - strand at 335.394 kb on + strand, within Shew_0309
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 10 mM remove 331,986 + -2.6 332,850 - Shew_0307 0.13 +0.5 333,130 - Shew_0307 0.28 +0.5 333,311 + Shew_0307 0.37 +0.8 333,319 + Shew_0307 0.37 +0.1 333,409 - Shew_0307 0.42 +0.7 333,447 + Shew_0307 0.44 -0.2 333,753 + Shew_0307 0.60 +0.0 333,838 + Shew_0307 0.64 +0.9 334,028 + Shew_0307 0.74 -0.8 334,074 + Shew_0307 0.77 +0.2 334,405 + +0.4 334,409 + -0.1 334,498 + +0.0 334,669 + Shew_0308 0.19 -0.2 334,783 - Shew_0308 0.35 +0.8 334,911 - Shew_0308 0.54 -0.0 334,940 - Shew_0308 0.58 +0.1 334,940 - Shew_0308 0.58 -0.0 334,974 + Shew_0308 0.62 -0.3 335,049 + Shew_0308 0.73 +0.6 335,058 - Shew_0308 0.74 +0.3 335,173 - -0.2 335,173 - -0.0 335,394 + Shew_0309 0.23 -0.0
Or see this region's nucleotide sequence