Experiment: Choline Lysine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0293 and Shew_0294 are separated by 393 nucleotides Shew_0294 and Shew_0295 are separated by 80 nucleotides Shew_0295 and Shew_0296 are separated by 378 nucleotides
Shew_0293: Shew_0293 - threonine dehydratase (RefSeq), at 313,397 to 314,947
_0293
Shew_0294: Shew_0294 - alanine--glyoxylate transaminase (RefSeq), at 315,341 to 316,483
_0294
Shew_0295: Shew_0295 - hypothetical protein (RefSeq), at 316,564 to 316,989
_0295
Shew_0296: Shew_0296 - heat shock protein Hsp20 (RefSeq), at 317,368 to 317,838
_0296
Position (kb) 315
316
317 Strain fitness (log2 ratio) -1
0
1 at 314.595 kb on - strand, within Shew_0293 at 314.753 kb on - strand, within Shew_0293 at 314.834 kb on - strand at 314.834 kb on - strand at 314.900 kb on - strand at 315.240 kb on - strand at 315.335 kb on + strand at 315.365 kb on - strand at 315.554 kb on + strand, within Shew_0294 at 315.593 kb on - strand, within Shew_0294 at 316.006 kb on + strand, within Shew_0294 at 316.123 kb on + strand, within Shew_0294 at 316.259 kb on + strand, within Shew_0294 at 316.460 kb on + strand at 316.593 kb on + strand at 316.598 kb on + strand at 316.603 kb on + strand at 316.697 kb on - strand, within Shew_0295 at 316.705 kb on + strand, within Shew_0295 at 316.878 kb on + strand, within Shew_0295 at 316.922 kb on - strand, within Shew_0295 at 317.028 kb on + strand at 317.213 kb on + strand at 317.223 kb on + strand at 317.223 kb on + strand at 317.294 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 10 mM remove 314,595 - Shew_0293 0.77 +0.4 314,753 - Shew_0293 0.87 +0.1 314,834 - -0.2 314,834 - -0.4 314,900 - +0.4 315,240 - -0.3 315,335 + -0.2 315,365 - -0.4 315,554 + Shew_0294 0.19 +0.4 315,593 - Shew_0294 0.22 +0.0 316,006 + Shew_0294 0.58 -0.1 316,123 + Shew_0294 0.68 -0.6 316,259 + Shew_0294 0.80 +0.2 316,460 + +0.2 316,593 + -0.1 316,598 + +0.0 316,603 + +0.0 316,697 - Shew_0295 0.31 +1.2 316,705 + Shew_0295 0.33 -0.2 316,878 + Shew_0295 0.74 +0.1 316,922 - Shew_0295 0.84 +0.5 317,028 + +0.3 317,213 + +0.3 317,223 + -1.1 317,223 + +0.3 317,294 + +0.1
Or see this region's nucleotide sequence