Experiment: KB with capsaicin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3678 and Psyr_3679 overlap by 4 nucleotides Psyr_3679 and Psyr_3680 overlap by 4 nucleotides Psyr_3680 and Psyr_3681 are separated by 25 nucleotides
Psyr_3678: Psyr_3678 - adenosylcobinamide-phosphate synthase, at 4,364,433 to 4,365,341
_3678
Psyr_3679: Psyr_3679 - cob(II)yrinic acid a,c-diamide reductase, at 4,365,338 to 4,365,988
_3679
Psyr_3680: Psyr_3680 - hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing) / cobyrinate a,c-diamide synthase, at 4,365,985 to 4,367,280
_3680
Psyr_3681: Psyr_3681 - cob(I)yrinic acid a,c-diamide adenosyltransferase, at 4,367,306 to 4,367,917
_3681
Position (kb) 4365
4366
4367
4368 Strain fitness (log2 ratio) -2
-1
0
1 at 4365.366 kb on + strand at 4366.154 kb on + strand, within Psyr_3680 at 4366.154 kb on + strand, within Psyr_3680 at 4366.154 kb on + strand, within Psyr_3680 at 4366.154 kb on + strand, within Psyr_3680 at 4366.301 kb on + strand, within Psyr_3680 at 4366.302 kb on - strand, within Psyr_3680 at 4366.427 kb on + strand, within Psyr_3680 at 4366.428 kb on - strand, within Psyr_3680 at 4366.428 kb on - strand, within Psyr_3680 at 4366.817 kb on - strand, within Psyr_3680 at 4367.429 kb on + strand, within Psyr_3681 at 4367.429 kb on + strand, within Psyr_3681 at 4367.430 kb on - strand, within Psyr_3681 at 4367.430 kb on - strand, within Psyr_3681 at 4367.430 kb on - strand, within Psyr_3681 at 4368.116 kb on + strand at 4368.116 kb on + strand at 4368.117 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with capsaicin 0.25 mg/ml remove 4,365,366 + -0.3 4,366,154 + Psyr_3680 0.13 -2.1 4,366,154 + Psyr_3680 0.13 -1.5 4,366,154 + Psyr_3680 0.13 -1.2 4,366,154 + Psyr_3680 0.13 -0.5 4,366,301 + Psyr_3680 0.24 -2.2 4,366,302 - Psyr_3680 0.24 -1.0 4,366,427 + Psyr_3680 0.34 -0.9 4,366,428 - Psyr_3680 0.34 -0.7 4,366,428 - Psyr_3680 0.34 -0.3 4,366,817 - Psyr_3680 0.64 -0.1 4,367,429 + Psyr_3681 0.20 -0.0 4,367,429 + Psyr_3681 0.20 -0.5 4,367,430 - Psyr_3681 0.20 +1.3 4,367,430 - Psyr_3681 0.20 +0.9 4,367,430 - Psyr_3681 0.20 -0.1 4,368,116 + +0.4 4,368,116 + -0.8 4,368,117 - +0.1
Or see this region's nucleotide sequence