Experiment: KB with capsaicin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2300 and Psyr_2301 overlap by 4 nucleotides Psyr_2301 and Psyr_2302 overlap by 4 nucleotides
Psyr_2300: Psyr_2300 - Conserved hypothetical protein 374, at 2,673,444 to 2,674,412
_2300
Psyr_2301: Psyr_2301 - Short-chain dehydrogenase/reductase SDR, at 2,674,409 to 2,675,149
_2301
Psyr_2302: Psyr_2302 - FAD linked oxidase, N-terminal, at 2,675,146 to 2,676,462
_2302
Position (kb)
2674
2675
2676 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2673.479 kb on + strand at 2673.537 kb on + strand at 2673.670 kb on + strand, within Psyr_2300 at 2673.674 kb on + strand, within Psyr_2300 at 2673.674 kb on + strand, within Psyr_2300 at 2673.678 kb on + strand, within Psyr_2300 at 2673.678 kb on + strand, within Psyr_2300 at 2673.849 kb on - strand, within Psyr_2300 at 2674.150 kb on + strand, within Psyr_2300 at 2674.150 kb on + strand, within Psyr_2300 at 2674.201 kb on + strand, within Psyr_2300 at 2674.201 kb on + strand, within Psyr_2300 at 2674.337 kb on - strand at 2674.622 kb on + strand, within Psyr_2301 at 2674.623 kb on - strand, within Psyr_2301 at 2674.623 kb on - strand, within Psyr_2301 at 2674.623 kb on - strand, within Psyr_2301 at 2674.623 kb on - strand, within Psyr_2301 at 2674.629 kb on - strand, within Psyr_2301 at 2674.704 kb on + strand, within Psyr_2301 at 2674.705 kb on - strand, within Psyr_2301 at 2674.983 kb on + strand, within Psyr_2301 at 2674.983 kb on + strand, within Psyr_2301 at 2674.984 kb on - strand, within Psyr_2301 at 2674.984 kb on - strand, within Psyr_2301 at 2674.984 kb on - strand, within Psyr_2301 at 2674.991 kb on + strand, within Psyr_2301 at 2674.991 kb on + strand, within Psyr_2301 at 2674.991 kb on + strand, within Psyr_2301 at 2674.992 kb on - strand, within Psyr_2301 at 2674.992 kb on - strand, within Psyr_2301 at 2674.992 kb on - strand, within Psyr_2301 at 2674.992 kb on - strand, within Psyr_2301 at 2675.123 kb on - strand at 2675.123 kb on - strand at 2675.580 kb on - strand, within Psyr_2302 at 2675.765 kb on + strand, within Psyr_2302 at 2675.765 kb on + strand, within Psyr_2302 at 2675.822 kb on + strand, within Psyr_2302 at 2675.822 kb on + strand, within Psyr_2302 at 2675.822 kb on + strand, within Psyr_2302 at 2675.856 kb on + strand, within Psyr_2302 at 2675.892 kb on + strand, within Psyr_2302 at 2675.892 kb on + strand, within Psyr_2302 at 2675.892 kb on + strand, within Psyr_2302 at 2676.100 kb on + strand, within Psyr_2302 at 2676.100 kb on + strand, within Psyr_2302 at 2676.100 kb on + strand, within Psyr_2302 at 2676.100 kb on + strand, within Psyr_2302 at 2676.100 kb on + strand, within Psyr_2302 at 2676.100 kb on + strand, within Psyr_2302 at 2676.100 kb on + strand, within Psyr_2302 at 2676.100 kb on + strand, within Psyr_2302 at 2676.100 kb on + strand, within Psyr_2302 at 2676.101 kb on - strand, within Psyr_2302 at 2676.135 kb on - strand, within Psyr_2302 at 2676.135 kb on - strand, within Psyr_2302 at 2676.135 kb on - strand, within Psyr_2302 at 2676.135 kb on - strand, within Psyr_2302
Per-strain Table
Position Strand Gene LocusTag Fraction KB with capsaicin 0.25 mg/ml remove 2,673,479 + +0.4 2,673,537 + -2.3 2,673,670 + Psyr_2300 0.23 +1.5 2,673,674 + Psyr_2300 0.24 +0.8 2,673,674 + Psyr_2300 0.24 -1.3 2,673,678 + Psyr_2300 0.24 +1.2 2,673,678 + Psyr_2300 0.24 +1.3 2,673,849 - Psyr_2300 0.42 +0.2 2,674,150 + Psyr_2300 0.73 +1.5 2,674,150 + Psyr_2300 0.73 +0.2 2,674,201 + Psyr_2300 0.78 +1.2 2,674,201 + Psyr_2300 0.78 -0.5 2,674,337 - +0.7 2,674,622 + Psyr_2301 0.29 +0.1 2,674,623 - Psyr_2301 0.29 -1.2 2,674,623 - Psyr_2301 0.29 -1.5 2,674,623 - Psyr_2301 0.29 -0.1 2,674,623 - Psyr_2301 0.29 -0.6 2,674,629 - Psyr_2301 0.30 -1.2 2,674,704 + Psyr_2301 0.40 +0.7 2,674,705 - Psyr_2301 0.40 -0.4 2,674,983 + Psyr_2301 0.77 -1.6 2,674,983 + Psyr_2301 0.77 -0.3 2,674,984 - Psyr_2301 0.78 -0.5 2,674,984 - Psyr_2301 0.78 +0.3 2,674,984 - Psyr_2301 0.78 -0.5 2,674,991 + Psyr_2301 0.79 -0.8 2,674,991 + Psyr_2301 0.79 +1.0 2,674,991 + Psyr_2301 0.79 +1.3 2,674,992 - Psyr_2301 0.79 -0.1 2,674,992 - Psyr_2301 0.79 +0.4 2,674,992 - Psyr_2301 0.79 -1.0 2,674,992 - Psyr_2301 0.79 -1.6 2,675,123 - -0.5 2,675,123 - -0.5 2,675,580 - Psyr_2302 0.33 +2.1 2,675,765 + Psyr_2302 0.47 +1.5 2,675,765 + Psyr_2302 0.47 +1.0 2,675,822 + Psyr_2302 0.51 +0.6 2,675,822 + Psyr_2302 0.51 -0.2 2,675,822 + Psyr_2302 0.51 -0.4 2,675,856 + Psyr_2302 0.54 -1.5 2,675,892 + Psyr_2302 0.57 -0.5 2,675,892 + Psyr_2302 0.57 +2.8 2,675,892 + Psyr_2302 0.57 -0.5 2,676,100 + Psyr_2302 0.72 +1.1 2,676,100 + Psyr_2302 0.72 -0.1 2,676,100 + Psyr_2302 0.72 -0.5 2,676,100 + Psyr_2302 0.72 +1.0 2,676,100 + Psyr_2302 0.72 +0.9 2,676,100 + Psyr_2302 0.72 -0.6 2,676,100 + Psyr_2302 0.72 +0.4 2,676,100 + Psyr_2302 0.72 -0.2 2,676,100 + Psyr_2302 0.72 +0.5 2,676,101 - Psyr_2302 0.73 -0.7 2,676,135 - Psyr_2302 0.75 -0.8 2,676,135 - Psyr_2302 0.75 +0.3 2,676,135 - Psyr_2302 0.75 +0.6 2,676,135 - Psyr_2302 0.75 +0.1
Or see this region's nucleotide sequence