Experiment: KB with capsaicin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2269 and Psyr_2270 are separated by 11 nucleotides Psyr_2270 and Psyr_2271 are separated by 140 nucleotides Psyr_2271 and Psyr_2272 are separated by 48 nucleotides Psyr_2272 and Psyr_2273 are separated by 288 nucleotides
Psyr_2269: Psyr_2269 - GTP cyclohydrolase I, at 2,635,090 to 2,635,995
_2269
Psyr_2270: Psyr_2270 - transferase hexapeptide repeat protein, at 2,636,007 to 2,636,567
_2270
Psyr_2271: Psyr_2271 - membrane protein, putative, at 2,636,708 to 2,637,874
_2271
Psyr_2272: Psyr_2272 - Helix-turn-helix motif protein, at 2,637,923 to 2,638,528
_2272
Psyr_2273: Psyr_2273 - L-glutamine synthetase, at 2,638,817 to 2,640,151
_2273
Position (kb)
2636
2637
2638 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2635.773 kb on - strand, within Psyr_2269 at 2635.861 kb on + strand, within Psyr_2269 at 2635.861 kb on + strand, within Psyr_2269 at 2635.861 kb on + strand, within Psyr_2269 at 2635.861 kb on + strand, within Psyr_2269 at 2635.984 kb on + strand at 2635.984 kb on + strand at 2635.985 kb on - strand at 2635.985 kb on - strand at 2636.059 kb on - strand at 2636.059 kb on - strand at 2636.067 kb on - strand, within Psyr_2270 at 2636.418 kb on + strand, within Psyr_2270 at 2636.418 kb on + strand, within Psyr_2270 at 2636.418 kb on + strand, within Psyr_2270 at 2636.418 kb on + strand, within Psyr_2270 at 2636.419 kb on - strand, within Psyr_2270 at 2636.419 kb on - strand, within Psyr_2270 at 2636.419 kb on - strand, within Psyr_2270 at 2636.419 kb on - strand, within Psyr_2270 at 2636.419 kb on - strand, within Psyr_2270 at 2636.419 kb on - strand, within Psyr_2270 at 2636.598 kb on + strand at 2636.600 kb on + strand at 2636.600 kb on + strand at 2636.600 kb on + strand at 2636.601 kb on - strand at 2636.601 kb on - strand at 2636.601 kb on - strand at 2636.613 kb on - strand at 2636.668 kb on + strand at 2636.669 kb on - strand at 2636.669 kb on - strand at 2636.782 kb on + strand at 2636.782 kb on + strand at 2636.963 kb on + strand, within Psyr_2271 at 2636.964 kb on - strand, within Psyr_2271 at 2637.080 kb on + strand, within Psyr_2271 at 2637.080 kb on + strand, within Psyr_2271 at 2637.080 kb on + strand, within Psyr_2271 at 2637.080 kb on + strand, within Psyr_2271 at 2637.081 kb on - strand, within Psyr_2271 at 2637.081 kb on - strand, within Psyr_2271 at 2637.298 kb on + strand, within Psyr_2271 at 2637.299 kb on - strand, within Psyr_2271 at 2637.636 kb on + strand, within Psyr_2271 at 2637.637 kb on - strand, within Psyr_2271 at 2637.637 kb on - strand, within Psyr_2271 at 2637.639 kb on + strand, within Psyr_2271 at 2637.680 kb on + strand, within Psyr_2271 at 2637.680 kb on + strand, within Psyr_2271 at 2637.680 kb on + strand, within Psyr_2271 at 2637.680 kb on + strand, within Psyr_2271 at 2637.681 kb on - strand, within Psyr_2271 at 2637.681 kb on - strand, within Psyr_2271 at 2637.681 kb on - strand, within Psyr_2271 at 2637.681 kb on - strand, within Psyr_2271 at 2637.741 kb on - strand, within Psyr_2271 at 2637.786 kb on + strand at 2637.866 kb on + strand at 2637.866 kb on + strand at 2637.873 kb on - strand at 2638.158 kb on + strand, within Psyr_2272 at 2638.158 kb on + strand, within Psyr_2272 at 2638.158 kb on + strand, within Psyr_2272 at 2638.158 kb on + strand, within Psyr_2272 at 2638.158 kb on + strand, within Psyr_2272 at 2638.159 kb on - strand, within Psyr_2272 at 2638.159 kb on - strand, within Psyr_2272 at 2638.159 kb on - strand, within Psyr_2272 at 2638.159 kb on - strand, within Psyr_2272 at 2638.207 kb on - strand, within Psyr_2272 at 2638.294 kb on - strand, within Psyr_2272 at 2638.483 kb on - strand at 2638.483 kb on - strand at 2638.617 kb on - strand at 2638.646 kb on + strand at 2638.647 kb on - strand at 2638.647 kb on - strand at 2638.647 kb on - strand at 2638.692 kb on - strand at 2638.749 kb on - strand at 2638.865 kb on + strand at 2638.865 kb on + strand at 2638.865 kb on + strand at 2638.865 kb on + strand at 2638.865 kb on + strand at 2638.865 kb on + strand at 2638.866 kb on - strand at 2638.869 kb on + strand at 2638.870 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with capsaicin 0.25 mg/ml remove 2,635,773 - Psyr_2269 0.75 +1.0 2,635,861 + Psyr_2269 0.85 +0.1 2,635,861 + Psyr_2269 0.85 -0.7 2,635,861 + Psyr_2269 0.85 +1.3 2,635,861 + Psyr_2269 0.85 +0.2 2,635,984 + -0.2 2,635,984 + -0.5 2,635,985 - +0.1 2,635,985 - +1.2 2,636,059 - +0.5 2,636,059 - +0.1 2,636,067 - Psyr_2270 0.11 -1.3 2,636,418 + Psyr_2270 0.73 -0.8 2,636,418 + Psyr_2270 0.73 -1.5 2,636,418 + Psyr_2270 0.73 +0.1 2,636,418 + Psyr_2270 0.73 -0.5 2,636,419 - Psyr_2270 0.73 -0.8 2,636,419 - Psyr_2270 0.73 +0.1 2,636,419 - Psyr_2270 0.73 -0.2 2,636,419 - Psyr_2270 0.73 -1.2 2,636,419 - Psyr_2270 0.73 +0.0 2,636,419 - Psyr_2270 0.73 +0.9 2,636,598 + -0.5 2,636,600 + +0.2 2,636,600 + +0.5 2,636,600 + -1.2 2,636,601 - -0.5 2,636,601 - -0.1 2,636,601 - -0.7 2,636,613 - +0.2 2,636,668 + +2.1 2,636,669 - -0.5 2,636,669 - -0.6 2,636,782 + -1.4 2,636,782 + +0.3 2,636,963 + Psyr_2271 0.22 -0.5 2,636,964 - Psyr_2271 0.22 -1.3 2,637,080 + Psyr_2271 0.32 -0.2 2,637,080 + Psyr_2271 0.32 -1.2 2,637,080 + Psyr_2271 0.32 -0.6 2,637,080 + Psyr_2271 0.32 +1.3 2,637,081 - Psyr_2271 0.32 -0.9 2,637,081 - Psyr_2271 0.32 -0.6 2,637,298 + Psyr_2271 0.51 +0.1 2,637,299 - Psyr_2271 0.51 -1.2 2,637,636 + Psyr_2271 0.80 -0.4 2,637,637 - Psyr_2271 0.80 -0.3 2,637,637 - Psyr_2271 0.80 -0.5 2,637,639 + Psyr_2271 0.80 +0.3 2,637,680 + Psyr_2271 0.83 +0.2 2,637,680 + Psyr_2271 0.83 -0.9 2,637,680 + Psyr_2271 0.83 -3.0 2,637,680 + Psyr_2271 0.83 -1.6 2,637,681 - Psyr_2271 0.83 -0.3 2,637,681 - Psyr_2271 0.83 -0.5 2,637,681 - Psyr_2271 0.83 -0.5 2,637,681 - Psyr_2271 0.83 -1.0 2,637,741 - Psyr_2271 0.89 +2.1 2,637,786 + +1.3 2,637,866 + -0.7 2,637,866 + +2.0 2,637,873 - -1.1 2,638,158 + Psyr_2272 0.39 +0.1 2,638,158 + Psyr_2272 0.39 +0.1 2,638,158 + Psyr_2272 0.39 +1.9 2,638,158 + Psyr_2272 0.39 -0.9 2,638,158 + Psyr_2272 0.39 +0.8 2,638,159 - Psyr_2272 0.39 +0.3 2,638,159 - Psyr_2272 0.39 +0.7 2,638,159 - Psyr_2272 0.39 -0.7 2,638,159 - Psyr_2272 0.39 -0.1 2,638,207 - Psyr_2272 0.47 +0.5 2,638,294 - Psyr_2272 0.61 -0.5 2,638,483 - -0.3 2,638,483 - -0.3 2,638,617 - +1.3 2,638,646 + -0.5 2,638,647 - -0.1 2,638,647 - +0.0 2,638,647 - +0.4 2,638,692 - +1.9 2,638,749 - +0.1 2,638,865 + +0.4 2,638,865 + -0.3 2,638,865 + -0.6 2,638,865 + +0.0 2,638,865 + +2.3 2,638,865 + +0.7 2,638,866 - +2.9 2,638,869 + +0.5 2,638,870 - -1.8
Or see this region's nucleotide sequence