Experiment: KB with capsaicin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2157 and Psyr_2158 are separated by 22 nucleotides Psyr_2158 and Psyr_2159 are separated by 309 nucleotides Psyr_2159 and Psyr_2160 are separated by 66 nucleotides Psyr_2160 and Psyr_2161 are separated by 295 nucleotides Psyr_2161 and Psyr_2162 are separated by 349 nucleotides
Psyr_2157: Psyr_2157 - Inosine/uridine-preferring nucleoside hydrolase, at 2,512,612 to 2,513,637
_2157
Psyr_2158: Psyr_2158 - conserved hypothetical protein, at 2,513,660 to 2,513,854
_2158
Psyr_2159: Psyr_2159 - lipoprotein, putative, at 2,514,164 to 2,514,505
_2159
Psyr_2160: Psyr_2160 - cold-shock DNA-binding protein family, at 2,514,572 to 2,514,784
_2160
Psyr_2161: Psyr_2161 - conserved hypothetical protein, at 2,515,080 to 2,515,385
_2161
Psyr_2162: Psyr_2162 - Ser-tRNA(Thr) hydrolase / threonyl-tRNA synthetase, at 2,515,735 to 2,517,657
_2162
Position (kb)
2514
2515 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2513.635 kb on + strand at 2513.635 kb on + strand at 2513.635 kb on + strand at 2513.635 kb on + strand at 2513.636 kb on - strand at 2513.636 kb on - strand at 2513.636 kb on - strand at 2513.639 kb on + strand at 2513.639 kb on + strand at 2513.640 kb on - strand at 2513.640 kb on - strand at 2513.640 kb on - strand at 2513.640 kb on - strand at 2513.686 kb on - strand, within Psyr_2158 at 2513.924 kb on - strand at 2513.957 kb on + strand at 2513.957 kb on + strand at 2513.958 kb on - strand at 2513.958 kb on - strand at 2513.958 kb on - strand at 2513.958 kb on - strand at 2513.961 kb on + strand at 2513.961 kb on + strand at 2513.961 kb on + strand at 2513.962 kb on - strand at 2513.962 kb on - strand at 2513.962 kb on - strand at 2514.163 kb on + strand at 2514.163 kb on + strand at 2514.164 kb on - strand at 2514.164 kb on - strand at 2514.193 kb on + strand at 2514.242 kb on - strand, within Psyr_2159 at 2514.616 kb on + strand, within Psyr_2160 at 2514.616 kb on + strand, within Psyr_2160 at 2514.689 kb on + strand, within Psyr_2160 at 2514.725 kb on - strand, within Psyr_2160 at 2514.759 kb on - strand, within Psyr_2160 at 2514.759 kb on - strand, within Psyr_2160 at 2514.789 kb on + strand at 2514.789 kb on + strand at 2514.790 kb on - strand at 2514.790 kb on - strand at 2514.790 kb on - strand at 2514.790 kb on - strand at 2514.849 kb on - strand at 2514.853 kb on + strand at 2514.854 kb on - strand at 2514.878 kb on + strand at 2514.878 kb on + strand at 2514.879 kb on - strand at 2514.879 kb on - strand at 2514.881 kb on + strand at 2514.910 kb on + strand at 2514.910 kb on + strand at 2514.910 kb on + strand at 2514.911 kb on - strand at 2514.911 kb on - strand at 2514.911 kb on - strand at 2514.912 kb on + strand at 2514.913 kb on - strand at 2514.913 kb on - strand at 2514.913 kb on - strand at 2514.927 kb on - strand at 2515.173 kb on + strand, within Psyr_2161 at 2515.174 kb on - strand, within Psyr_2161 at 2515.397 kb on + strand at 2515.520 kb on + strand at 2515.594 kb on + strand at 2515.719 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with capsaicin 0.25 mg/ml remove 2,513,635 + +1.5 2,513,635 + +0.7 2,513,635 + +1.1 2,513,635 + -0.8 2,513,636 - +2.5 2,513,636 - +1.8 2,513,636 - +0.2 2,513,639 + -0.8 2,513,639 + +0.1 2,513,640 - -0.2 2,513,640 - +0.9 2,513,640 - -3.1 2,513,640 - -2.1 2,513,686 - Psyr_2158 0.13 -0.3 2,513,924 - -0.2 2,513,957 + -0.3 2,513,957 + +2.3 2,513,958 - -0.6 2,513,958 - -0.8 2,513,958 - -1.5 2,513,958 - +1.5 2,513,961 + +0.1 2,513,961 + +2.1 2,513,961 + +1.7 2,513,962 - +0.1 2,513,962 - -0.7 2,513,962 - +0.3 2,514,163 + +0.5 2,514,163 + -1.0 2,514,164 - -0.5 2,514,164 - +0.6 2,514,193 + +1.0 2,514,242 - Psyr_2159 0.23 -0.2 2,514,616 + Psyr_2160 0.21 -0.4 2,514,616 + Psyr_2160 0.21 -0.5 2,514,689 + Psyr_2160 0.55 -0.6 2,514,725 - Psyr_2160 0.72 -0.5 2,514,759 - Psyr_2160 0.88 +1.5 2,514,759 - Psyr_2160 0.88 -0.9 2,514,789 + -1.1 2,514,789 + -2.5 2,514,790 - -1.1 2,514,790 - -0.2 2,514,790 - -0.5 2,514,790 - -2.3 2,514,849 - +1.0 2,514,853 + -1.3 2,514,854 - -0.4 2,514,878 + -0.2 2,514,878 + +0.4 2,514,879 - +1.3 2,514,879 - +0.5 2,514,881 + +1.1 2,514,910 + -0.5 2,514,910 + -1.1 2,514,910 + -0.2 2,514,911 - -1.1 2,514,911 - +1.6 2,514,911 - +3.1 2,514,912 + -0.9 2,514,913 - +0.5 2,514,913 - -0.2 2,514,913 - +0.3 2,514,927 - -0.7 2,515,173 + Psyr_2161 0.30 +1.2 2,515,174 - Psyr_2161 0.31 -2.1 2,515,397 + -0.7 2,515,520 + -0.2 2,515,594 + +0.6 2,515,719 + -2.1
Or see this region's nucleotide sequence