Experiment: KB with capsaicin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1106 and Psyr_1107 are separated by 59 nucleotides Psyr_1107 and Psyr_1108 are separated by 137 nucleotides Psyr_1108 and Psyr_1109 are separated by 219 nucleotides
Psyr_1106: Psyr_1106 - FecR protein, at 1,253,132 to 1,254,097
_1106
Psyr_1107: Psyr_1107 - Sigma-70 region 2, at 1,254,157 to 1,254,720
_1107
Psyr_1108: Psyr_1108 - glyceraldehyde-3-phosphate dehydrogenase, at 1,254,858 to 1,255,859
_1108
Psyr_1109: Psyr_1109 - 6-phosphogluconate dehydratase, at 1,256,079 to 1,257,905
_1109
Position (kb)
1254
1255
1256 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1254.124 kb on + strand at 1254.131 kb on - strand at 1254.188 kb on - strand at 1254.188 kb on - strand at 1254.188 kb on - strand at 1254.188 kb on - strand at 1254.223 kb on + strand, within Psyr_1107 at 1254.223 kb on + strand, within Psyr_1107 at 1254.223 kb on + strand, within Psyr_1107 at 1254.224 kb on - strand, within Psyr_1107 at 1254.266 kb on + strand, within Psyr_1107 at 1254.267 kb on - strand, within Psyr_1107 at 1254.267 kb on - strand, within Psyr_1107 at 1254.267 kb on - strand, within Psyr_1107 at 1254.618 kb on + strand, within Psyr_1107 at 1254.618 kb on + strand, within Psyr_1107 at 1254.618 kb on + strand, within Psyr_1107 at 1254.618 kb on + strand, within Psyr_1107 at 1254.619 kb on - strand, within Psyr_1107 at 1254.619 kb on - strand, within Psyr_1107 at 1254.622 kb on + strand, within Psyr_1107 at 1254.622 kb on + strand, within Psyr_1107 at 1254.622 kb on + strand, within Psyr_1107 at 1254.622 kb on + strand, within Psyr_1107 at 1254.622 kb on + strand, within Psyr_1107 at 1254.622 kb on + strand, within Psyr_1107 at 1254.623 kb on - strand, within Psyr_1107 at 1254.623 kb on - strand, within Psyr_1107 at 1254.623 kb on - strand, within Psyr_1107 at 1254.749 kb on - strand at 1254.752 kb on + strand at 1254.752 kb on + strand at 1254.752 kb on + strand at 1254.752 kb on + strand at 1254.752 kb on + strand at 1254.753 kb on - strand at 1254.753 kb on - strand at 1254.753 kb on - strand at 1254.753 kb on - strand at 1254.753 kb on - strand at 1254.859 kb on + strand at 1254.936 kb on + strand at 1254.937 kb on - strand at 1254.937 kb on - strand at 1254.937 kb on - strand at 1254.937 kb on - strand at 1254.937 kb on - strand at 1255.037 kb on - strand, within Psyr_1108 at 1255.197 kb on + strand, within Psyr_1108 at 1255.282 kb on + strand, within Psyr_1108 at 1255.282 kb on + strand, within Psyr_1108 at 1255.283 kb on - strand, within Psyr_1108 at 1255.312 kb on + strand, within Psyr_1108 at 1255.313 kb on - strand, within Psyr_1108 at 1255.313 kb on - strand, within Psyr_1108 at 1255.313 kb on - strand, within Psyr_1108 at 1255.365 kb on + strand, within Psyr_1108 at 1255.365 kb on + strand, within Psyr_1108 at 1255.365 kb on + strand, within Psyr_1108 at 1255.366 kb on - strand, within Psyr_1108 at 1255.366 kb on - strand, within Psyr_1108 at 1255.366 kb on - strand, within Psyr_1108 at 1255.366 kb on - strand, within Psyr_1108 at 1255.456 kb on + strand, within Psyr_1108 at 1255.457 kb on - strand, within Psyr_1108 at 1255.457 kb on - strand, within Psyr_1108 at 1255.457 kb on - strand, within Psyr_1108 at 1255.457 kb on - strand, within Psyr_1108 at 1255.496 kb on + strand, within Psyr_1108 at 1255.497 kb on - strand, within Psyr_1108 at 1255.581 kb on + strand, within Psyr_1108 at 1255.581 kb on + strand, within Psyr_1108 at 1255.581 kb on + strand, within Psyr_1108 at 1255.582 kb on - strand, within Psyr_1108 at 1255.582 kb on - strand, within Psyr_1108 at 1255.582 kb on - strand, within Psyr_1108 at 1255.582 kb on - strand, within Psyr_1108 at 1255.582 kb on - strand, within Psyr_1108 at 1255.582 kb on - strand, within Psyr_1108 at 1255.582 kb on - strand, within Psyr_1108 at 1255.582 kb on - strand, within Psyr_1108 at 1255.637 kb on + strand, within Psyr_1108 at 1255.637 kb on + strand, within Psyr_1108 at 1255.638 kb on - strand, within Psyr_1108 at 1255.701 kb on + strand, within Psyr_1108 at 1255.702 kb on - strand, within Psyr_1108 at 1255.702 kb on - strand, within Psyr_1108 at 1255.702 kb on - strand, within Psyr_1108 at 1255.702 kb on - strand, within Psyr_1108 at 1255.794 kb on - strand at 1255.795 kb on + strand at 1255.795 kb on + strand at 1255.795 kb on + strand at 1255.795 kb on + strand at 1255.795 kb on + strand at 1255.796 kb on - strand at 1255.796 kb on - strand at 1255.796 kb on - strand at 1255.796 kb on - strand at 1255.796 kb on - strand at 1255.796 kb on - strand at 1255.796 kb on - strand at 1255.796 kb on - strand at 1255.806 kb on + strand at 1255.806 kb on + strand at 1255.859 kb on + strand at 1255.859 kb on + strand at 1255.860 kb on - strand at 1255.860 kb on - strand at 1255.959 kb on + strand at 1255.960 kb on - strand at 1256.055 kb on - strand at 1256.062 kb on - strand at 1256.101 kb on + strand at 1256.146 kb on - strand at 1256.298 kb on + strand, within Psyr_1109 at 1256.298 kb on + strand, within Psyr_1109 at 1256.298 kb on + strand, within Psyr_1109 at 1256.299 kb on - strand, within Psyr_1109 at 1256.299 kb on - strand, within Psyr_1109 at 1256.362 kb on + strand, within Psyr_1109 at 1256.363 kb on - strand, within Psyr_1109 at 1256.363 kb on - strand, within Psyr_1109 at 1256.363 kb on - strand, within Psyr_1109 at 1256.507 kb on + strand, within Psyr_1109 at 1256.507 kb on + strand, within Psyr_1109 at 1256.507 kb on + strand, within Psyr_1109 at 1256.507 kb on + strand, within Psyr_1109 at 1256.508 kb on - strand, within Psyr_1109 at 1256.508 kb on - strand, within Psyr_1109 at 1256.508 kb on - strand, within Psyr_1109 at 1256.508 kb on - strand, within Psyr_1109 at 1256.508 kb on - strand, within Psyr_1109 at 1256.508 kb on - strand, within Psyr_1109 at 1256.656 kb on + strand, within Psyr_1109 at 1256.657 kb on - strand, within Psyr_1109 at 1256.828 kb on + strand, within Psyr_1109 at 1256.828 kb on + strand, within Psyr_1109 at 1256.828 kb on + strand, within Psyr_1109 at 1256.828 kb on + strand, within Psyr_1109 at 1256.828 kb on + strand, within Psyr_1109 at 1256.829 kb on - strand, within Psyr_1109
Per-strain Table
Position Strand Gene LocusTag Fraction KB with capsaicin 0.25 mg/ml remove 1,254,124 + +0.0 1,254,131 - -1.2 1,254,188 - +1.3 1,254,188 - -0.5 1,254,188 - +0.0 1,254,188 - -0.8 1,254,223 + Psyr_1107 0.12 +1.0 1,254,223 + Psyr_1107 0.12 -0.2 1,254,223 + Psyr_1107 0.12 +0.3 1,254,224 - Psyr_1107 0.12 -1.4 1,254,266 + Psyr_1107 0.19 +0.6 1,254,267 - Psyr_1107 0.20 +0.7 1,254,267 - Psyr_1107 0.20 -0.4 1,254,267 - Psyr_1107 0.20 +0.5 1,254,618 + Psyr_1107 0.82 +0.5 1,254,618 + Psyr_1107 0.82 -1.0 1,254,618 + Psyr_1107 0.82 -0.2 1,254,618 + Psyr_1107 0.82 -0.7 1,254,619 - Psyr_1107 0.82 +0.1 1,254,619 - Psyr_1107 0.82 +0.6 1,254,622 + Psyr_1107 0.82 -1.4 1,254,622 + Psyr_1107 0.82 -0.8 1,254,622 + Psyr_1107 0.82 +0.8 1,254,622 + Psyr_1107 0.82 -0.5 1,254,622 + Psyr_1107 0.82 -0.2 1,254,622 + Psyr_1107 0.82 -1.2 1,254,623 - Psyr_1107 0.83 +0.6 1,254,623 - Psyr_1107 0.83 +0.0 1,254,623 - Psyr_1107 0.83 -1.1 1,254,749 - -0.4 1,254,752 + -0.9 1,254,752 + +0.6 1,254,752 + -0.4 1,254,752 + +1.0 1,254,752 + -1.7 1,254,753 - -1.1 1,254,753 - +0.3 1,254,753 - +2.4 1,254,753 - -1.8 1,254,753 - -0.3 1,254,859 + +0.7 1,254,936 + +2.0 1,254,937 - +0.4 1,254,937 - -2.8 1,254,937 - -0.5 1,254,937 - -0.7 1,254,937 - -0.6 1,255,037 - Psyr_1108 0.18 -1.1 1,255,197 + Psyr_1108 0.34 -1.2 1,255,282 + Psyr_1108 0.42 -0.2 1,255,282 + Psyr_1108 0.42 +0.8 1,255,283 - Psyr_1108 0.42 -0.7 1,255,312 + Psyr_1108 0.45 -0.8 1,255,313 - Psyr_1108 0.45 -1.5 1,255,313 - Psyr_1108 0.45 -1.4 1,255,313 - Psyr_1108 0.45 -0.4 1,255,365 + Psyr_1108 0.51 -1.3 1,255,365 + Psyr_1108 0.51 -0.5 1,255,365 + Psyr_1108 0.51 -1.5 1,255,366 - Psyr_1108 0.51 -2.3 1,255,366 - Psyr_1108 0.51 +2.8 1,255,366 - Psyr_1108 0.51 -1.4 1,255,366 - Psyr_1108 0.51 +0.3 1,255,456 + Psyr_1108 0.60 -1.3 1,255,457 - Psyr_1108 0.60 -1.9 1,255,457 - Psyr_1108 0.60 +0.1 1,255,457 - Psyr_1108 0.60 -0.7 1,255,457 - Psyr_1108 0.60 -1.2 1,255,496 + Psyr_1108 0.64 -0.8 1,255,497 - Psyr_1108 0.64 -1.6 1,255,581 + Psyr_1108 0.72 +1.8 1,255,581 + Psyr_1108 0.72 -1.3 1,255,581 + Psyr_1108 0.72 -0.2 1,255,582 - Psyr_1108 0.72 -0.6 1,255,582 - Psyr_1108 0.72 -0.4 1,255,582 - Psyr_1108 0.72 -0.1 1,255,582 - Psyr_1108 0.72 -1.3 1,255,582 - Psyr_1108 0.72 -0.5 1,255,582 - Psyr_1108 0.72 -1.5 1,255,582 - Psyr_1108 0.72 -1.0 1,255,582 - Psyr_1108 0.72 -1.3 1,255,637 + Psyr_1108 0.78 +0.0 1,255,637 + Psyr_1108 0.78 -0.1 1,255,638 - Psyr_1108 0.78 -2.0 1,255,701 + Psyr_1108 0.84 -1.8 1,255,702 - Psyr_1108 0.84 -1.2 1,255,702 - Psyr_1108 0.84 -2.1 1,255,702 - Psyr_1108 0.84 -0.2 1,255,702 - Psyr_1108 0.84 +1.2 1,255,794 - +0.0 1,255,795 + -0.2 1,255,795 + +0.2 1,255,795 + -0.1 1,255,795 + -1.5 1,255,795 + -1.1 1,255,796 - -1.6 1,255,796 - -2.2 1,255,796 - -2.0 1,255,796 - -0.6 1,255,796 - -1.5 1,255,796 - -0.5 1,255,796 - -0.5 1,255,796 - -0.5 1,255,806 + -1.4 1,255,806 + -0.9 1,255,859 + -0.8 1,255,859 + +0.2 1,255,860 - -0.5 1,255,860 - -1.0 1,255,959 + -0.3 1,255,960 - +1.3 1,256,055 - +0.3 1,256,062 - +0.8 1,256,101 + +0.6 1,256,146 - +0.1 1,256,298 + Psyr_1109 0.12 -0.6 1,256,298 + Psyr_1109 0.12 +0.1 1,256,298 + Psyr_1109 0.12 -2.0 1,256,299 - Psyr_1109 0.12 +0.5 1,256,299 - Psyr_1109 0.12 +1.1 1,256,362 + Psyr_1109 0.15 -1.1 1,256,363 - Psyr_1109 0.16 -0.7 1,256,363 - Psyr_1109 0.16 +1.2 1,256,363 - Psyr_1109 0.16 +0.3 1,256,507 + Psyr_1109 0.23 +1.2 1,256,507 + Psyr_1109 0.23 -0.6 1,256,507 + Psyr_1109 0.23 +0.8 1,256,507 + Psyr_1109 0.23 -0.5 1,256,508 - Psyr_1109 0.23 -0.6 1,256,508 - Psyr_1109 0.23 -0.1 1,256,508 - Psyr_1109 0.23 +0.5 1,256,508 - Psyr_1109 0.23 -0.5 1,256,508 - Psyr_1109 0.23 -0.8 1,256,508 - Psyr_1109 0.23 -0.9 1,256,656 + Psyr_1109 0.32 -0.7 1,256,657 - Psyr_1109 0.32 -1.0 1,256,828 + Psyr_1109 0.41 -0.2 1,256,828 + Psyr_1109 0.41 +1.8 1,256,828 + Psyr_1109 0.41 -0.9 1,256,828 + Psyr_1109 0.41 -1.9 1,256,828 + Psyr_1109 0.41 -0.9 1,256,829 - Psyr_1109 0.41 +0.8
Or see this region's nucleotide sequence