Experiment: KB with capsaicin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0182 and Psyr_0183 are separated by 5 nucleotides Psyr_0183 and Psyr_0184 are separated by 13 nucleotides Psyr_0184 and Psyr_0185 are separated by 2 nucleotides Psyr_0185 and Psyr_0186 overlap by 8 nucleotides
Psyr_0182: Psyr_0182 - diaminopimelate decarboxylase, at 195,060 to 196,307
_0182
Psyr_0183: Psyr_0183 - diaminopimelate epimerase, at 196,313 to 197,143
_0183
Psyr_0184: Psyr_0184 - Protein of unknown function DUF484, at 197,157 to 197,891
_0184
Psyr_0185: Psyr_0185 - tyrosine recombinase XerC subunit, at 197,894 to 198,793
_0185
Psyr_0186: Psyr_0186 - HAD-superfamily hydrolase, subfamily IA, variant 3:HAD-superfamily hydrolase, subfamily IA, variant 1, at 198,786 to 199,478
_0186
Position (kb) 197
198 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 197.149 kb on + strand at 197.149 kb on + strand at 197.149 kb on + strand at 197.153 kb on + strand at 197.153 kb on + strand at 197.153 kb on + strand at 197.153 kb on + strand at 197.154 kb on - strand at 197.154 kb on - strand at 197.154 kb on - strand at 197.154 kb on - strand at 197.154 kb on - strand at 197.154 kb on - strand at 197.154 kb on - strand at 197.154 kb on - strand at 197.793 kb on + strand, within Psyr_0184 at 197.793 kb on + strand, within Psyr_0184 at 197.793 kb on + strand, within Psyr_0184 at 197.794 kb on - strand, within Psyr_0184 at 197.794 kb on - strand, within Psyr_0184 at 197.826 kb on + strand at 197.826 kb on + strand at 197.826 kb on + strand at 197.826 kb on + strand at 197.826 kb on + strand at 197.826 kb on + strand at 197.827 kb on - strand at 197.827 kb on - strand at 197.827 kb on - strand at 197.827 kb on - strand at 197.827 kb on - strand at 197.827 kb on - strand at 197.827 kb on - strand at 197.827 kb on - strand at 197.827 kb on - strand at 197.828 kb on + strand at 197.875 kb on + strand at 197.915 kb on + strand at 197.916 kb on - strand at 197.916 kb on - strand at 197.916 kb on - strand at 197.916 kb on - strand at 197.999 kb on + strand, within Psyr_0185 at 198.000 kb on - strand, within Psyr_0185 at 198.134 kb on + strand, within Psyr_0185 at 198.134 kb on + strand, within Psyr_0185 at 198.134 kb on + strand, within Psyr_0185 at 198.135 kb on - strand, within Psyr_0185 at 198.135 kb on - strand, within Psyr_0185 at 198.135 kb on - strand, within Psyr_0185 at 198.135 kb on - strand, within Psyr_0185 at 198.135 kb on - strand, within Psyr_0185 at 198.135 kb on - strand, within Psyr_0185 at 198.135 kb on - strand, within Psyr_0185 at 198.140 kb on + strand, within Psyr_0185 at 198.140 kb on + strand, within Psyr_0185 at 198.140 kb on + strand, within Psyr_0185 at 198.141 kb on - strand, within Psyr_0185 at 198.141 kb on - strand, within Psyr_0185 at 198.141 kb on - strand, within Psyr_0185 at 198.420 kb on + strand, within Psyr_0185 at 198.691 kb on + strand, within Psyr_0185 at 198.691 kb on + strand, within Psyr_0185 at 198.692 kb on - strand, within Psyr_0185 at 198.692 kb on - strand, within Psyr_0185 at 198.705 kb on + strand at 198.705 kb on + strand at 198.705 kb on + strand at 198.705 kb on + strand at 198.705 kb on + strand at 198.705 kb on + strand at 198.705 kb on + strand at 198.705 kb on + strand at 198.705 kb on + strand at 198.705 kb on + strand at 198.705 kb on + strand at 198.705 kb on + strand at 198.706 kb on - strand at 198.706 kb on - strand at 198.706 kb on - strand at 198.706 kb on - strand at 198.706 kb on - strand at 198.706 kb on - strand at 198.707 kb on + strand at 198.707 kb on + strand at 198.707 kb on + strand at 198.707 kb on + strand at 198.707 kb on + strand at 198.707 kb on + strand at 198.707 kb on + strand at 198.707 kb on + strand at 198.707 kb on + strand at 198.707 kb on + strand at 198.707 kb on + strand at 198.707 kb on + strand at 198.707 kb on + strand at 198.707 kb on + strand at 198.707 kb on + strand at 198.707 kb on + strand at 198.707 kb on + strand at 198.707 kb on + strand at 198.707 kb on + strand at 198.707 kb on + strand at 198.707 kb on + strand at 198.707 kb on + strand at 198.707 kb on + strand at 198.707 kb on + strand at 198.707 kb on + strand at 198.708 kb on - strand at 198.708 kb on - strand at 198.708 kb on - strand at 198.708 kb on - strand at 198.708 kb on - strand at 198.708 kb on - strand at 198.708 kb on - strand at 198.708 kb on - strand at 198.708 kb on - strand at 198.708 kb on - strand at 198.708 kb on - strand at 198.708 kb on - strand at 198.708 kb on - strand at 198.708 kb on - strand at 198.708 kb on - strand at 198.708 kb on - strand at 198.708 kb on - strand at 198.708 kb on - strand at 198.708 kb on - strand at 198.708 kb on - strand at 198.708 kb on - strand at 198.708 kb on - strand at 198.708 kb on - strand at 198.708 kb on - strand at 198.708 kb on - strand at 198.708 kb on - strand at 198.708 kb on - strand at 198.708 kb on - strand at 198.708 kb on - strand at 198.708 kb on - strand at 198.708 kb on - strand at 198.709 kb on + strand at 198.709 kb on + strand at 198.709 kb on + strand at 198.709 kb on + strand at 198.709 kb on + strand at 198.709 kb on + strand at 198.709 kb on + strand at 198.709 kb on + strand at 198.709 kb on + strand at 198.709 kb on + strand at 198.709 kb on + strand at 198.709 kb on + strand at 198.709 kb on + strand at 198.709 kb on + strand at 198.709 kb on + strand at 198.709 kb on + strand at 198.709 kb on + strand at 198.709 kb on + strand at 198.709 kb on + strand at 198.709 kb on + strand at 198.709 kb on + strand at 198.709 kb on + strand at 198.709 kb on + strand at 198.709 kb on + strand at 198.709 kb on + strand at 198.709 kb on + strand at 198.709 kb on + strand at 198.709 kb on + strand at 198.709 kb on + strand at 198.709 kb on + strand at 198.710 kb on - strand at 198.710 kb on - strand at 198.710 kb on - strand at 198.710 kb on - strand at 198.710 kb on - strand at 198.710 kb on - strand at 198.710 kb on - strand at 198.710 kb on - strand at 198.710 kb on - strand at 198.710 kb on - strand at 198.710 kb on - strand at 198.710 kb on - strand at 198.710 kb on - strand at 198.710 kb on - strand at 198.710 kb on - strand at 198.710 kb on - strand at 198.710 kb on - strand at 198.710 kb on - strand at 198.710 kb on - strand at 198.710 kb on - strand at 198.710 kb on - strand at 198.710 kb on - strand at 198.710 kb on - strand at 198.710 kb on - strand at 198.710 kb on - strand at 198.710 kb on - strand at 198.710 kb on - strand at 198.710 kb on - strand at 198.710 kb on - strand at 198.710 kb on - strand at 198.710 kb on - strand at 198.710 kb on - strand at 198.710 kb on - strand at 198.710 kb on - strand at 198.710 kb on - strand at 198.710 kb on - strand at 198.743 kb on + strand at 198.744 kb on - strand at 198.744 kb on - strand at 198.772 kb on + strand at 198.772 kb on + strand at 198.772 kb on + strand at 198.773 kb on - strand at 198.773 kb on - strand at 198.791 kb on + strand at 198.792 kb on - strand at 198.792 kb on - strand at 198.792 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with capsaicin 0.25 mg/ml remove 197,149 + -0.4 197,149 + +0.6 197,149 + -2.2 197,153 + -0.3 197,153 + -0.1 197,153 + +0.4 197,153 + -1.2 197,154 - -1.0 197,154 - +1.2 197,154 - -0.5 197,154 - -0.8 197,154 - -0.6 197,154 - -1.6 197,154 - -0.9 197,154 - -0.3 197,793 + Psyr_0184 0.87 -0.2 197,793 + Psyr_0184 0.87 -0.5 197,793 + Psyr_0184 0.87 -1.0 197,794 - Psyr_0184 0.87 -3.5 197,794 - Psyr_0184 0.87 -1.6 197,826 + -0.5 197,826 + -0.2 197,826 + -1.0 197,826 + +0.5 197,826 + -0.1 197,826 + +0.4 197,827 - -1.7 197,827 - -1.9 197,827 - -1.5 197,827 - +1.8 197,827 - -0.0 197,827 - -1.9 197,827 - -0.5 197,827 - -1.4 197,827 - -0.6 197,828 + +0.2 197,875 + +0.1 197,915 + +0.0 197,916 - -1.9 197,916 - -1.5 197,916 - -0.6 197,916 - +0.1 197,999 + Psyr_0185 0.12 -0.4 198,000 - Psyr_0185 0.12 -1.6 198,134 + Psyr_0185 0.27 -0.7 198,134 + Psyr_0185 0.27 -0.4 198,134 + Psyr_0185 0.27 -0.4 198,135 - Psyr_0185 0.27 +0.2 198,135 - Psyr_0185 0.27 -1.3 198,135 - Psyr_0185 0.27 -0.4 198,135 - Psyr_0185 0.27 -0.2 198,135 - Psyr_0185 0.27 -0.7 198,135 - Psyr_0185 0.27 -0.2 198,135 - Psyr_0185 0.27 -1.5 198,140 + Psyr_0185 0.27 +0.1 198,140 + Psyr_0185 0.27 -2.5 198,140 + Psyr_0185 0.27 -0.6 198,141 - Psyr_0185 0.27 +0.3 198,141 - Psyr_0185 0.27 +0.2 198,141 - Psyr_0185 0.27 -1.1 198,420 + Psyr_0185 0.58 +1.0 198,691 + Psyr_0185 0.89 +0.1 198,691 + Psyr_0185 0.89 -0.6 198,692 - Psyr_0185 0.89 +0.6 198,692 - Psyr_0185 0.89 +0.2 198,705 + -0.0 198,705 + -0.7 198,705 + +0.8 198,705 + -1.8 198,705 + -0.8 198,705 + -0.6 198,705 + -3.4 198,705 + -0.7 198,705 + -0.1 198,705 + +0.7 198,705 + -0.2 198,705 + -0.4 198,706 - -0.4 198,706 - -1.2 198,706 - +1.2 198,706 - +0.2 198,706 - +0.4 198,706 - +0.0 198,707 + -0.3 198,707 + -0.7 198,707 + -0.3 198,707 + -1.1 198,707 + +0.7 198,707 + +1.0 198,707 + -1.1 198,707 + -1.2 198,707 + -0.4 198,707 + -1.0 198,707 + -0.1 198,707 + -2.7 198,707 + +0.0 198,707 + +0.2 198,707 + -0.8 198,707 + +0.5 198,707 + +0.6 198,707 + -1.7 198,707 + -0.6 198,707 + -0.1 198,707 + +1.0 198,707 + +0.4 198,707 + +0.5 198,707 + +1.4 198,707 + +0.7 198,708 - -0.4 198,708 - +0.4 198,708 - -1.4 198,708 - -0.4 198,708 - -0.4 198,708 - -0.2 198,708 - -0.5 198,708 - -0.5 198,708 - -0.3 198,708 - -0.1 198,708 - -0.1 198,708 - +1.2 198,708 - -1.1 198,708 - -1.8 198,708 - -0.4 198,708 - +1.5 198,708 - +1.2 198,708 - -0.2 198,708 - -0.7 198,708 - -0.4 198,708 - -1.9 198,708 - -0.7 198,708 - -0.6 198,708 - -2.8 198,708 - -0.1 198,708 - -1.2 198,708 - -0.4 198,708 - -0.0 198,708 - -0.8 198,708 - -1.6 198,708 - -1.0 198,709 + -0.2 198,709 + +0.2 198,709 + -0.3 198,709 + -0.8 198,709 + +0.4 198,709 + +0.2 198,709 + -1.3 198,709 + -1.6 198,709 + -0.6 198,709 + +0.0 198,709 + -0.7 198,709 + -0.5 198,709 + +1.0 198,709 + -0.6 198,709 + -0.1 198,709 + +0.0 198,709 + +2.3 198,709 + -2.4 198,709 + +1.2 198,709 + +0.3 198,709 + -1.0 198,709 + -0.4 198,709 + -0.9 198,709 + -1.1 198,709 + -1.7 198,709 + +0.1 198,709 + +0.1 198,709 + +0.9 198,709 + +0.4 198,709 + -0.3 198,710 - +0.1 198,710 - -2.2 198,710 - +0.4 198,710 - +0.5 198,710 - +0.5 198,710 - -0.4 198,710 - +0.1 198,710 - -0.1 198,710 - +1.1 198,710 - -0.3 198,710 - -0.1 198,710 - -0.6 198,710 - -0.5 198,710 - -3.1 198,710 - +0.2 198,710 - +0.5 198,710 - -0.0 198,710 - +0.1 198,710 - -1.3 198,710 - -0.9 198,710 - -0.6 198,710 - -0.4 198,710 - -0.4 198,710 - -1.9 198,710 - -0.1 198,710 - -0.9 198,710 - -0.6 198,710 - -0.6 198,710 - -1.1 198,710 - +2.2 198,710 - -0.4 198,710 - +0.4 198,710 - -0.9 198,710 - +0.9 198,710 - -2.2 198,710 - +0.7 198,743 + +0.4 198,744 - -0.3 198,744 - -1.9 198,772 + -1.9 198,772 + -0.9 198,772 + +2.9 198,773 - -0.2 198,773 - -0.8 198,791 + +0.8 198,792 - +0.2 198,792 - -0.5 198,792 - +1.2
Or see this region's nucleotide sequence