Experiment: KB with capsaicin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0043 and Psyr_0044 are separated by 171 nucleotides Psyr_0044 and Psyr_0045 are separated by 112 nucleotides
Psyr_0043: Psyr_0043 - conserved hypothetical protein, at 44,004 to 45,059
_0043
Psyr_0044: Psyr_0044 - transferase hexapeptide repeat protein, at 45,231 to 45,776
_0044
Psyr_0045: Psyr_0045 - oligopeptidase A, Metallo peptidase, MEROPS family M03A, at 45,889 to 47,940
_0045
Position (kb)
45
46 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 44.268 kb on + strand, within Psyr_0043 at 44.269 kb on - strand, within Psyr_0043 at 44.289 kb on + strand, within Psyr_0043 at 44.289 kb on + strand, within Psyr_0043 at 44.289 kb on + strand, within Psyr_0043 at 44.289 kb on + strand, within Psyr_0043 at 44.289 kb on + strand, within Psyr_0043 at 44.289 kb on + strand, within Psyr_0043 at 44.290 kb on - strand, within Psyr_0043 at 44.290 kb on - strand, within Psyr_0043 at 44.290 kb on - strand, within Psyr_0043 at 44.290 kb on - strand, within Psyr_0043 at 44.290 kb on - strand, within Psyr_0043 at 44.290 kb on - strand, within Psyr_0043 at 44.383 kb on - strand, within Psyr_0043 at 44.385 kb on + strand, within Psyr_0043 at 44.385 kb on + strand, within Psyr_0043 at 44.386 kb on - strand, within Psyr_0043 at 44.445 kb on + strand, within Psyr_0043 at 44.445 kb on + strand, within Psyr_0043 at 44.445 kb on + strand, within Psyr_0043 at 44.446 kb on - strand, within Psyr_0043 at 44.446 kb on - strand, within Psyr_0043 at 44.446 kb on - strand, within Psyr_0043 at 44.446 kb on - strand, within Psyr_0043 at 44.446 kb on - strand, within Psyr_0043 at 44.446 kb on - strand, within Psyr_0043 at 44.446 kb on - strand, within Psyr_0043 at 44.446 kb on - strand, within Psyr_0043 at 44.466 kb on + strand, within Psyr_0043 at 44.574 kb on + strand, within Psyr_0043 at 44.574 kb on + strand, within Psyr_0043 at 44.575 kb on - strand, within Psyr_0043 at 44.575 kb on - strand, within Psyr_0043 at 44.607 kb on + strand, within Psyr_0043 at 44.897 kb on + strand, within Psyr_0043 at 44.898 kb on - strand, within Psyr_0043 at 44.919 kb on + strand, within Psyr_0043 at 44.919 kb on + strand, within Psyr_0043 at 44.920 kb on - strand, within Psyr_0043 at 44.985 kb on + strand at 45.216 kb on + strand at 45.217 kb on - strand at 45.217 kb on - strand at 45.219 kb on + strand at 45.219 kb on + strand at 45.219 kb on + strand at 45.219 kb on + strand at 45.219 kb on + strand at 45.220 kb on - strand at 45.220 kb on - strand at 45.220 kb on - strand at 45.220 kb on - strand at 45.220 kb on - strand at 45.239 kb on + strand at 45.280 kb on + strand at 45.280 kb on + strand at 45.280 kb on + strand at 45.280 kb on + strand at 45.280 kb on + strand at 45.280 kb on + strand at 45.280 kb on + strand at 45.280 kb on + strand at 45.280 kb on + strand at 45.280 kb on + strand at 45.280 kb on + strand at 45.280 kb on + strand at 45.280 kb on + strand at 45.281 kb on - strand at 45.281 kb on - strand at 45.281 kb on - strand at 45.281 kb on - strand at 45.281 kb on - strand at 45.281 kb on - strand at 45.285 kb on - strand at 45.295 kb on + strand, within Psyr_0044 at 45.358 kb on + strand, within Psyr_0044 at 45.358 kb on + strand, within Psyr_0044 at 45.779 kb on + strand at 45.780 kb on - strand at 45.801 kb on + strand at 45.801 kb on + strand at 45.801 kb on + strand at 45.809 kb on + strand at 45.809 kb on + strand at 45.809 kb on + strand at 45.845 kb on + strand at 45.845 kb on + strand at 45.846 kb on - strand at 45.849 kb on + strand at 45.850 kb on - strand at 45.850 kb on - strand at 45.850 kb on - strand at 45.850 kb on - strand at 45.850 kb on - strand at 45.850 kb on - strand at 45.850 kb on - strand at 46.153 kb on + strand, within Psyr_0045 at 46.153 kb on + strand, within Psyr_0045 at 46.154 kb on - strand, within Psyr_0045 at 46.154 kb on - strand, within Psyr_0045 at 46.225 kb on + strand, within Psyr_0045 at 46.226 kb on - strand, within Psyr_0045 at 46.226 kb on - strand, within Psyr_0045 at 46.525 kb on + strand, within Psyr_0045 at 46.526 kb on - strand, within Psyr_0045 at 46.552 kb on + strand, within Psyr_0045 at 46.552 kb on + strand, within Psyr_0045 at 46.552 kb on + strand, within Psyr_0045 at 46.552 kb on + strand, within Psyr_0045 at 46.552 kb on + strand, within Psyr_0045 at 46.552 kb on + strand, within Psyr_0045 at 46.552 kb on + strand, within Psyr_0045 at 46.553 kb on - strand, within Psyr_0045 at 46.553 kb on - strand, within Psyr_0045 at 46.553 kb on - strand, within Psyr_0045 at 46.556 kb on - strand, within Psyr_0045 at 46.556 kb on - strand, within Psyr_0045 at 46.570 kb on + strand, within Psyr_0045 at 46.571 kb on - strand, within Psyr_0045 at 46.571 kb on - strand, within Psyr_0045 at 46.603 kb on + strand, within Psyr_0045 at 46.603 kb on + strand, within Psyr_0045 at 46.729 kb on + strand, within Psyr_0045 at 46.729 kb on + strand, within Psyr_0045 at 46.729 kb on + strand, within Psyr_0045 at 46.729 kb on + strand, within Psyr_0045 at 46.729 kb on + strand, within Psyr_0045 at 46.729 kb on + strand, within Psyr_0045
Per-strain Table
Position Strand Gene LocusTag Fraction KB with capsaicin 0.25 mg/ml remove 44,268 + Psyr_0043 0.25 -0.2 44,269 - Psyr_0043 0.25 -0.4 44,289 + Psyr_0043 0.27 +1.0 44,289 + Psyr_0043 0.27 -1.9 44,289 + Psyr_0043 0.27 -1.9 44,289 + Psyr_0043 0.27 -0.3 44,289 + Psyr_0043 0.27 +0.2 44,289 + Psyr_0043 0.27 +1.0 44,290 - Psyr_0043 0.27 -0.3 44,290 - Psyr_0043 0.27 +0.5 44,290 - Psyr_0043 0.27 +0.1 44,290 - Psyr_0043 0.27 -1.1 44,290 - Psyr_0043 0.27 -1.1 44,290 - Psyr_0043 0.27 -2.3 44,383 - Psyr_0043 0.36 +1.2 44,385 + Psyr_0043 0.36 +0.2 44,385 + Psyr_0043 0.36 -0.8 44,386 - Psyr_0043 0.36 +0.7 44,445 + Psyr_0043 0.42 -0.2 44,445 + Psyr_0043 0.42 -0.2 44,445 + Psyr_0043 0.42 -0.4 44,446 - Psyr_0043 0.42 +0.7 44,446 - Psyr_0043 0.42 +1.2 44,446 - Psyr_0043 0.42 -0.7 44,446 - Psyr_0043 0.42 +0.7 44,446 - Psyr_0043 0.42 -0.5 44,446 - Psyr_0043 0.42 +0.4 44,446 - Psyr_0043 0.42 -1.2 44,446 - Psyr_0043 0.42 +0.1 44,466 + Psyr_0043 0.44 +0.1 44,574 + Psyr_0043 0.54 +0.4 44,574 + Psyr_0043 0.54 +0.6 44,575 - Psyr_0043 0.54 -0.3 44,575 - Psyr_0043 0.54 +0.3 44,607 + Psyr_0043 0.57 +0.4 44,897 + Psyr_0043 0.85 +0.6 44,898 - Psyr_0043 0.85 -0.3 44,919 + Psyr_0043 0.87 +0.1 44,919 + Psyr_0043 0.87 -0.1 44,920 - Psyr_0043 0.87 -0.1 44,985 + -0.1 45,216 + -0.6 45,217 - +0.1 45,217 - +0.1 45,219 + -0.0 45,219 + -0.4 45,219 + -0.1 45,219 + +0.5 45,219 + -0.2 45,220 - +0.3 45,220 - -0.0 45,220 - -2.1 45,220 - +0.9 45,220 - +0.5 45,239 + -0.3 45,280 + -0.8 45,280 + +0.3 45,280 + -0.2 45,280 + -0.2 45,280 + -0.1 45,280 + +0.4 45,280 + +0.6 45,280 + +0.6 45,280 + +0.4 45,280 + -1.3 45,280 + -0.2 45,280 + -0.6 45,280 + -0.1 45,281 - -0.3 45,281 - -0.3 45,281 - +0.0 45,281 - -3.6 45,281 - +0.5 45,281 - -0.5 45,285 - +0.0 45,295 + Psyr_0044 0.12 -1.3 45,358 + Psyr_0044 0.23 +0.7 45,358 + Psyr_0044 0.23 +1.2 45,779 + -0.5 45,780 - +0.2 45,801 + +0.2 45,801 + -1.6 45,801 + -0.3 45,809 + +0.2 45,809 + +1.0 45,809 + +2.7 45,845 + +0.7 45,845 + -0.6 45,846 - -0.4 45,849 + +0.5 45,850 - +0.0 45,850 - -0.4 45,850 - -0.2 45,850 - -0.7 45,850 - -0.5 45,850 - +0.9 45,850 - +0.0 46,153 + Psyr_0045 0.13 -0.8 46,153 + Psyr_0045 0.13 +0.2 46,154 - Psyr_0045 0.13 -1.2 46,154 - Psyr_0045 0.13 +1.0 46,225 + Psyr_0045 0.16 +1.1 46,226 - Psyr_0045 0.16 -0.8 46,226 - Psyr_0045 0.16 +0.2 46,525 + Psyr_0045 0.31 +0.2 46,526 - Psyr_0045 0.31 +1.0 46,552 + Psyr_0045 0.32 +0.1 46,552 + Psyr_0045 0.32 +0.2 46,552 + Psyr_0045 0.32 +1.7 46,552 + Psyr_0045 0.32 +0.7 46,552 + Psyr_0045 0.32 +0.2 46,552 + Psyr_0045 0.32 -0.5 46,552 + Psyr_0045 0.32 +0.5 46,553 - Psyr_0045 0.32 +0.4 46,553 - Psyr_0045 0.32 +0.2 46,553 - Psyr_0045 0.32 +1.2 46,556 - Psyr_0045 0.33 -0.3 46,556 - Psyr_0045 0.33 +0.5 46,570 + Psyr_0045 0.33 +0.2 46,571 - Psyr_0045 0.33 +1.2 46,571 - Psyr_0045 0.33 +0.5 46,603 + Psyr_0045 0.35 -0.1 46,603 + Psyr_0045 0.35 +0.4 46,729 + Psyr_0045 0.41 -0.4 46,729 + Psyr_0045 0.41 +0.0 46,729 + Psyr_0045 0.41 +2.8 46,729 + Psyr_0045 0.41 +2.2 46,729 + Psyr_0045 0.41 -0.1 46,729 + Psyr_0045 0.41 -1.2
Or see this region's nucleotide sequence