Experiment: KB with capsaicin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0035 and Psyr_0036 are separated by 0 nucleotides Psyr_0036 and Psyr_0037 are separated by 165 nucleotides Psyr_0037 and Psyr_0038 are separated by 150 nucleotides Psyr_0038 and Psyr_0039 are separated by 137 nucleotides
Psyr_0035: Psyr_0035 - regulatory protein, LysR:LysR, substrate-binding protein, at 39,307 to 40,263
_0035
Psyr_0036: Psyr_0036 - protein of unknown function DUF883, at 40,264 to 40,590
_0036
Psyr_0037: Psyr_0037 - Protein of unknown function DUF1458, at 40,756 to 40,971
_0037
Psyr_0038: Psyr_0038 - Protein of unknown function DUF1161, at 41,122 to 41,346
_0038
Psyr_0039: Psyr_0039 - luciferase, at 41,484 to 42,485
_0039
Position (kb)
41
42 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 40.289 kb on + strand at 40.289 kb on + strand at 40.289 kb on + strand at 40.289 kb on + strand at 40.289 kb on + strand at 40.289 kb on + strand at 40.289 kb on + strand at 40.289 kb on + strand at 40.289 kb on + strand at 40.289 kb on + strand at 40.290 kb on - strand at 40.290 kb on - strand at 40.290 kb on - strand at 40.290 kb on - strand at 40.290 kb on - strand at 40.290 kb on - strand at 40.290 kb on - strand at 40.290 kb on - strand at 40.355 kb on + strand, within Psyr_0036 at 40.356 kb on - strand, within Psyr_0036 at 40.356 kb on - strand, within Psyr_0036 at 40.406 kb on + strand, within Psyr_0036 at 40.406 kb on + strand, within Psyr_0036 at 40.407 kb on - strand, within Psyr_0036 at 40.407 kb on - strand, within Psyr_0036 at 40.407 kb on - strand, within Psyr_0036 at 40.407 kb on - strand, within Psyr_0036 at 40.407 kb on - strand, within Psyr_0036 at 40.552 kb on - strand, within Psyr_0036 at 40.552 kb on - strand, within Psyr_0036 at 40.581 kb on + strand at 40.581 kb on + strand at 40.581 kb on + strand at 40.581 kb on + strand at 40.581 kb on + strand at 40.581 kb on + strand at 40.581 kb on + strand at 40.581 kb on + strand at 40.582 kb on - strand at 40.582 kb on - strand at 40.582 kb on - strand at 40.582 kb on - strand at 40.582 kb on - strand at 40.582 kb on - strand at 40.582 kb on - strand at 40.582 kb on - strand at 40.582 kb on - strand at 40.584 kb on + strand at 40.584 kb on + strand at 40.720 kb on + strand at 40.720 kb on + strand at 40.720 kb on + strand at 40.720 kb on + strand at 40.721 kb on - strand at 40.721 kb on - strand at 40.721 kb on - strand at 40.721 kb on - strand at 40.722 kb on + strand at 40.774 kb on + strand at 40.774 kb on + strand at 40.774 kb on + strand at 40.774 kb on + strand at 40.774 kb on + strand at 40.774 kb on + strand at 40.774 kb on + strand at 40.775 kb on - strand at 40.775 kb on - strand at 40.775 kb on - strand at 40.934 kb on + strand, within Psyr_0037 at 40.934 kb on + strand, within Psyr_0037 at 40.935 kb on - strand, within Psyr_0037 at 40.969 kb on + strand at 40.969 kb on + strand at 40.970 kb on - strand at 40.970 kb on - strand at 41.006 kb on + strand at 41.016 kb on + strand at 41.017 kb on - strand at 41.017 kb on - strand at 41.030 kb on + strand at 41.030 kb on + strand at 41.144 kb on + strand at 41.248 kb on + strand, within Psyr_0038 at 41.248 kb on + strand, within Psyr_0038 at 41.248 kb on + strand, within Psyr_0038 at 41.248 kb on + strand, within Psyr_0038 at 41.248 kb on + strand, within Psyr_0038 at 41.248 kb on + strand, within Psyr_0038 at 41.249 kb on - strand, within Psyr_0038 at 41.249 kb on - strand, within Psyr_0038 at 41.515 kb on + strand at 41.542 kb on + strand at 41.542 kb on + strand at 41.542 kb on + strand at 41.542 kb on + strand at 41.542 kb on + strand at 41.542 kb on + strand at 41.542 kb on + strand at 41.542 kb on + strand at 41.542 kb on + strand at 41.543 kb on - strand at 41.543 kb on - strand at 41.543 kb on - strand at 41.543 kb on - strand at 41.543 kb on - strand at 41.543 kb on - strand at 41.543 kb on - strand at 41.543 kb on - strand at 41.543 kb on - strand at 41.543 kb on - strand at 41.543 kb on - strand at 41.543 kb on - strand at 41.543 kb on - strand at 41.543 kb on - strand at 41.543 kb on - strand at 41.555 kb on + strand at 41.655 kb on + strand, within Psyr_0039 at 41.756 kb on - strand, within Psyr_0039 at 41.774 kb on - strand, within Psyr_0039 at 41.774 kb on - strand, within Psyr_0039 at 41.774 kb on - strand, within Psyr_0039 at 41.827 kb on + strand, within Psyr_0039 at 41.828 kb on - strand, within Psyr_0039 at 41.828 kb on - strand, within Psyr_0039 at 41.828 kb on - strand, within Psyr_0039 at 41.869 kb on + strand, within Psyr_0039 at 41.893 kb on + strand, within Psyr_0039 at 41.894 kb on - strand, within Psyr_0039 at 41.894 kb on - strand, within Psyr_0039 at 42.049 kb on + strand, within Psyr_0039 at 42.049 kb on + strand, within Psyr_0039 at 42.049 kb on + strand, within Psyr_0039 at 42.050 kb on - strand, within Psyr_0039
Per-strain Table
Position Strand Gene LocusTag Fraction KB with capsaicin 0.25 mg/ml remove 40,289 + +0.9 40,289 + -0.8 40,289 + +1.1 40,289 + -1.2 40,289 + +1.5 40,289 + -0.2 40,289 + +0.2 40,289 + -0.8 40,289 + -0.5 40,289 + -0.9 40,290 - +1.0 40,290 - -0.6 40,290 - -0.6 40,290 - +0.9 40,290 - +0.4 40,290 - -0.8 40,290 - +0.7 40,290 - +0.3 40,355 + Psyr_0036 0.28 -0.5 40,356 - Psyr_0036 0.28 -0.3 40,356 - Psyr_0036 0.28 +0.5 40,406 + Psyr_0036 0.43 -1.3 40,406 + Psyr_0036 0.43 +0.8 40,407 - Psyr_0036 0.44 -0.2 40,407 - Psyr_0036 0.44 +2.1 40,407 - Psyr_0036 0.44 +1.7 40,407 - Psyr_0036 0.44 +0.4 40,407 - Psyr_0036 0.44 -0.8 40,552 - Psyr_0036 0.88 +0.4 40,552 - Psyr_0036 0.88 +1.0 40,581 + +1.3 40,581 + -0.3 40,581 + -0.3 40,581 + -0.1 40,581 + -0.0 40,581 + -0.3 40,581 + -0.0 40,581 + +1.2 40,582 - -0.3 40,582 - -0.2 40,582 - -0.3 40,582 - -0.9 40,582 - +0.5 40,582 - -0.6 40,582 - -0.3 40,582 - +0.2 40,582 - +3.1 40,584 + +1.9 40,584 + +2.2 40,720 + -0.1 40,720 + -1.0 40,720 + -0.5 40,720 + +0.1 40,721 - +0.2 40,721 - -1.1 40,721 - +1.8 40,721 - +1.4 40,722 + +0.6 40,774 + +0.3 40,774 + +1.8 40,774 + -0.9 40,774 + +0.5 40,774 + +0.5 40,774 + +0.0 40,774 + -0.3 40,775 - -0.6 40,775 - -0.8 40,775 - +0.2 40,934 + Psyr_0037 0.82 -0.8 40,934 + Psyr_0037 0.82 +0.9 40,935 - Psyr_0037 0.83 -1.2 40,969 + -1.2 40,969 + -0.0 40,970 - -0.3 40,970 - +0.4 41,006 + +1.4 41,016 + +0.3 41,017 - -1.8 41,017 - -1.4 41,030 + -1.1 41,030 + -0.0 41,144 + +0.2 41,248 + Psyr_0038 0.56 +0.8 41,248 + Psyr_0038 0.56 +0.7 41,248 + Psyr_0038 0.56 +0.2 41,248 + Psyr_0038 0.56 +1.1 41,248 + Psyr_0038 0.56 +0.7 41,248 + Psyr_0038 0.56 +1.1 41,249 - Psyr_0038 0.56 +0.8 41,249 - Psyr_0038 0.56 +0.7 41,515 + -1.2 41,542 + +0.1 41,542 + +0.9 41,542 + -0.7 41,542 + +1.2 41,542 + +0.7 41,542 + +1.0 41,542 + +2.2 41,542 + -0.4 41,542 + -0.5 41,543 - -0.1 41,543 - -0.5 41,543 - -0.3 41,543 - -0.3 41,543 - +0.1 41,543 - +0.6 41,543 - -0.5 41,543 - -0.8 41,543 - +0.3 41,543 - +0.2 41,543 - +1.2 41,543 - -0.9 41,543 - -0.4 41,543 - +0.1 41,543 - +0.1 41,555 + -0.4 41,655 + Psyr_0039 0.17 +0.2 41,756 - Psyr_0039 0.27 -0.8 41,774 - Psyr_0039 0.29 -0.3 41,774 - Psyr_0039 0.29 +0.1 41,774 - Psyr_0039 0.29 +0.9 41,827 + Psyr_0039 0.34 -1.1 41,828 - Psyr_0039 0.34 -0.9 41,828 - Psyr_0039 0.34 -0.2 41,828 - Psyr_0039 0.34 -2.2 41,869 + Psyr_0039 0.38 -1.6 41,893 + Psyr_0039 0.41 -0.1 41,894 - Psyr_0039 0.41 -0.1 41,894 - Psyr_0039 0.41 -1.3 42,049 + Psyr_0039 0.56 +0.7 42,049 + Psyr_0039 0.56 +0.3 42,049 + Psyr_0039 0.56 -0.3 42,050 - Psyr_0039 0.56 +0.4
Or see this region's nucleotide sequence