Experiment: H2 N-free with 10mM D-glucosamine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS08610 and MMJJ_RS08615 overlap by 14 nucleotides MMJJ_RS08615 and MMJJ_RS08620 are separated by 28 nucleotides
MMJJ_RS08610: MMJJ_RS08610 - oligosaccharide repeat unit polymerase family protein, at 1,585,396 to 1,586,469
_RS08610
MMJJ_RS08615: MMJJ_RS08615 - oligosaccharide repeat unit polymerase family protein, at 1,586,456 to 1,587,631
_RS08615
MMJJ_RS08620: MMJJ_RS08620 - DUF373 family protein, at 1,587,660 to 1,588,769
_RS08620
Position (kb)
1586
1587
1588 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1585.726 kb on + strand, within MMJJ_RS08610 at 1586.417 kb on - strand at 1586.417 kb on - strand at 1586.533 kb on + strand at 1586.628 kb on - strand, within MMJJ_RS08615 at 1586.629 kb on + strand, within MMJJ_RS08615 at 1586.691 kb on - strand, within MMJJ_RS08615 at 1586.775 kb on - strand, within MMJJ_RS08615 at 1586.805 kb on - strand, within MMJJ_RS08615 at 1586.806 kb on + strand, within MMJJ_RS08615 at 1586.838 kb on + strand, within MMJJ_RS08615 at 1586.871 kb on - strand, within MMJJ_RS08615 at 1586.881 kb on + strand, within MMJJ_RS08615 at 1586.881 kb on + strand, within MMJJ_RS08615 at 1586.905 kb on + strand, within MMJJ_RS08615 at 1586.905 kb on + strand, within MMJJ_RS08615 at 1586.967 kb on - strand, within MMJJ_RS08615 at 1587.020 kb on - strand, within MMJJ_RS08615 at 1587.031 kb on + strand, within MMJJ_RS08615 at 1587.081 kb on - strand, within MMJJ_RS08615 at 1587.082 kb on + strand, within MMJJ_RS08615 at 1587.103 kb on - strand, within MMJJ_RS08615 at 1587.153 kb on - strand, within MMJJ_RS08615 at 1587.154 kb on + strand, within MMJJ_RS08615 at 1587.157 kb on - strand at 1587.273 kb on - strand, within MMJJ_RS08615 at 1587.274 kb on + strand, within MMJJ_RS08615 at 1587.283 kb on - strand, within MMJJ_RS08615 at 1587.286 kb on + strand, within MMJJ_RS08615 at 1587.323 kb on + strand, within MMJJ_RS08615 at 1587.354 kb on - strand, within MMJJ_RS08615 at 1587.354 kb on - strand, within MMJJ_RS08615 at 1587.373 kb on - strand, within MMJJ_RS08615 at 1587.374 kb on + strand, within MMJJ_RS08615 at 1587.374 kb on + strand, within MMJJ_RS08615 at 1587.374 kb on + strand, within MMJJ_RS08615 at 1587.376 kb on - strand, within MMJJ_RS08615 at 1587.377 kb on + strand, within MMJJ_RS08615 at 1587.380 kb on + strand, within MMJJ_RS08615 at 1587.405 kb on + strand, within MMJJ_RS08615 at 1587.429 kb on - strand, within MMJJ_RS08615 at 1587.445 kb on + strand, within MMJJ_RS08615 at 1587.445 kb on + strand, within MMJJ_RS08615 at 1587.460 kb on - strand, within MMJJ_RS08615 at 1587.465 kb on - strand, within MMJJ_RS08615 at 1587.466 kb on + strand, within MMJJ_RS08615 at 1587.471 kb on + strand, within MMJJ_RS08615 at 1587.514 kb on + strand at 1587.659 kb on + strand at 1587.671 kb on + strand at 1587.886 kb on - strand, within MMJJ_RS08620 at 1587.952 kb on - strand, within MMJJ_RS08620 at 1588.008 kb on + strand, within MMJJ_RS08620 at 1588.326 kb on + strand, within MMJJ_RS08620 at 1588.402 kb on - strand at 1588.408 kb on - strand, within MMJJ_RS08620 at 1588.445 kb on + strand, within MMJJ_RS08620 at 1588.475 kb on - strand, within MMJJ_RS08620 at 1588.476 kb on + strand, within MMJJ_RS08620 at 1588.528 kb on - strand, within MMJJ_RS08620 at 1588.540 kb on - strand, within MMJJ_RS08620
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM D-glucosamine remove 1,585,726 + MMJJ_RS08610 0.31 -1.5 1,586,417 - +0.3 1,586,417 - -0.4 1,586,533 + -2.0 1,586,628 - MMJJ_RS08615 0.15 -0.7 1,586,629 + MMJJ_RS08615 0.15 -2.6 1,586,691 - MMJJ_RS08615 0.20 -2.2 1,586,775 - MMJJ_RS08615 0.27 -1.2 1,586,805 - MMJJ_RS08615 0.30 -3.2 1,586,806 + MMJJ_RS08615 0.30 -3.1 1,586,838 + MMJJ_RS08615 0.32 -0.5 1,586,871 - MMJJ_RS08615 0.35 -0.7 1,586,881 + MMJJ_RS08615 0.36 -1.2 1,586,881 + MMJJ_RS08615 0.36 -2.6 1,586,905 + MMJJ_RS08615 0.38 -1.9 1,586,905 + MMJJ_RS08615 0.38 -1.7 1,586,967 - MMJJ_RS08615 0.43 -1.2 1,587,020 - MMJJ_RS08615 0.48 -0.5 1,587,031 + MMJJ_RS08615 0.49 -1.6 1,587,081 - MMJJ_RS08615 0.53 -1.2 1,587,082 + MMJJ_RS08615 0.53 -1.6 1,587,103 - MMJJ_RS08615 0.55 -1.9 1,587,153 - MMJJ_RS08615 0.59 -2.8 1,587,154 + MMJJ_RS08615 0.59 -3.7 1,587,157 - +1.4 1,587,273 - MMJJ_RS08615 0.69 -2.1 1,587,274 + MMJJ_RS08615 0.70 +0.3 1,587,283 - MMJJ_RS08615 0.70 -2.0 1,587,286 + MMJJ_RS08615 0.71 -3.0 1,587,323 + MMJJ_RS08615 0.74 -2.0 1,587,354 - MMJJ_RS08615 0.76 -1.3 1,587,354 - MMJJ_RS08615 0.76 -1.7 1,587,373 - MMJJ_RS08615 0.78 -2.3 1,587,374 + MMJJ_RS08615 0.78 -1.6 1,587,374 + MMJJ_RS08615 0.78 -1.0 1,587,374 + MMJJ_RS08615 0.78 -0.5 1,587,376 - MMJJ_RS08615 0.78 -2.4 1,587,377 + MMJJ_RS08615 0.78 -2.0 1,587,380 + MMJJ_RS08615 0.79 -1.0 1,587,405 + MMJJ_RS08615 0.81 -1.6 1,587,429 - MMJJ_RS08615 0.83 +0.1 1,587,445 + MMJJ_RS08615 0.84 -2.0 1,587,445 + MMJJ_RS08615 0.84 -1.6 1,587,460 - MMJJ_RS08615 0.85 -2.6 1,587,465 - MMJJ_RS08615 0.86 -3.7 1,587,466 + MMJJ_RS08615 0.86 -2.7 1,587,471 + MMJJ_RS08615 0.86 -2.1 1,587,514 + -0.7 1,587,659 + +1.1 1,587,671 + -1.3 1,587,886 - MMJJ_RS08620 0.20 -1.3 1,587,952 - MMJJ_RS08620 0.26 -0.5 1,588,008 + MMJJ_RS08620 0.31 -1.2 1,588,326 + MMJJ_RS08620 0.60 -0.7 1,588,402 - -1.0 1,588,408 - MMJJ_RS08620 0.67 -3.3 1,588,445 + MMJJ_RS08620 0.71 -1.0 1,588,475 - MMJJ_RS08620 0.73 +0.8 1,588,476 + MMJJ_RS08620 0.74 -0.9 1,588,528 - MMJJ_RS08620 0.78 -4.1 1,588,540 - MMJJ_RS08620 0.79 -0.1
Or see this region's nucleotide sequence